2026-10-19T01:52:42Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/request

oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/101212026-10-18T21:13:08Zopenairedrivercom_ebyu_10000col_ebyu_10001

Effects of Prebiotic Gum Arabic Under Antibiotic-Containing Conditions in Atopic Dermatitis-Associated Bacteria: In Vitro Evaluation and Development of Semisolid Topical CarriersDoğanay, DeryaMertoğlu, EsraKurt, Ahmet ArifÖzkan, Batuhan CenkBursalıoğlu, Ertuğrul OsmanBozyel, Mustafa ErayAliusta, ReyhanTürkoğlu, ÖzlemGökçe, Halise BetülKızılay, EmineHacımustafaoğlu, FatihKalay, ŞabanHamdemir, RanaBayır, IsmailAslan, Ismailacacia gumantibioticsatopic dermatitisdysbiosisgum arabicIVRTprobioticsrheologyStaphylococcus aureusBackground/Objectives: Atopic dermatitis (AD) is associated with gut dysbiosis linked to early-life antibiotic use and Staphylococcus aureus colonization. Gum Arabic (GA), a prebiotic, may modulate this dysbiosis and influence AD-related microbial balance. This study evaluated whether GA could support AD-associated probiotics-Lactobacillus casei, Bifidobacterium bifidum, and Bifidobacterium infantis-under amoxicillin- or azithromycin-containing conditions, examined the response of S. aureus under the same screening conditions, and developed GA-phospholipid-based semisolid carriers for topical application. Methods: Probiotic strains were cultured with 1–5% GA in the presence and absence of antibiotics, and viable cell counts were assessed. Sixteen topical formulations containing propylene glycol or isopropyl myristate in a hydrogenated phosphatidylcholine base were prepared and screened for rheological properties and galactose release using in vitro release testing (IVRT) and HPLC-UV. Results: GA at 1–2% concentrations promoted probiotic growth in antibiotic-free conditions. GA preserved B. infantis viability under azithromycin exposure in this in vitro screening model. For S. aureus, numerical CFU differences were observed between antibiotic-only and GA-containing conditions; however, the present screening design was not intended to determine antibiotic interaction outcomes. Formulations F14 (2% GA + 7% IPM) and F15 (3% GA + 7% IPM) exhibited optimal spreadability. IVRT showed that 6 h cumulative galactose release varied by formulation (F6 > F10 > F14 > F15). Conclusions: GA demonstrated dose-dependent prebiotic activity and preserved B. infantis viability under azithromycin exposure in this in vitro screening model. For S. aureus, the observed CFU differences between antibiotic-only and GA-containing conditions should be considered exploratory only and do not allow for conclusions regarding interference with antibiotic efficacy. Optimized GA-HPC systems with suitable rheological and release characteristics represent promising candidates for further preclinical investigation. © 2026 by the authors.Multidisciplinary Digital Publishing Institute (MDPI)2026Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/10121https://doi.org/10.3390/antibiotics150403782079-6382enAntibioticsinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/10121