2026-10-18T23:54:18Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/request

oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/101282026-10-18T21:13:07Zopenairedrivercom_ebyu_10000col_ebyu_10001

Protective effects of multi-strain probiotics against polystyrene nanoplastic-induced parotid gland toxicity: role of NRF2/HO-1 and NF-κB pathwaysKozan, GünayÜstündağ, HilalKara, AdeminflammationnanoplasticsNrf2 pathwayoxidative stressparotid glandpolystyreneprobioticsaquaporin 5heme oxygenase 1immunoglobulin enhancer binding proteinketaminenanoplasticprobiotic agentprotein bcl 2transcription factor Nrf2xylazineacinar celladultanimal experimentanimal modelanimal tissueantioxidant activityArticlebacterial strainBifidobacteriumBifidobacterium longumcontrolled studydisease severitydown regulationhistopathologyBackground – Polystyrene nanoplastics (NPs) represent emerging environmental contaminants with potential adverse effects on salivary glands. This study investigated the protective effects of multi-strain probiotics against NPs-induced toxicity in rat parotid glands. Methods – Forty male Sprague-Dawley rats were allocated into five groups (n = 8): control, NPs (10 mg/kg), probiotics (1010 CFU), NPs + probiotics low dose (109 CFU), and NPs + Pro high dose (1010 CFU). Treatments were administered orally for 35 days. Oxidative stress markers, histopathological changes, and protein expression were evaluated. Results – NPs exposure significantly increased MDA levels (p < 0.05) and decreased SOD activity and GSH content (p < 0.05). Histopathological examination revealed moderate to severe acinar degeneration (++) and fibrosis (++) in NPs-treated animals. Western blot analysis showed significant downregulation of Bcl-2 (p < 0.01), HO-1 (p < 0.0001), NRF2 (p < 0.01), and AQP5 (p < 0.0001), with upregulation of Caspase-3 (p < 0.0001), NF-κB (p < 0.001), IL-1β (p < 0.001), and TNF-α (p < 0.001). Probiotic supplementation significantly ameliorated oxidative stress (p < 0.05), reduced histopathological damage to mild changes (+), and restored beneficial protein expression while suppressing inflammatory markers (p < 0.01-0.001). Conclusion – This study provides the first evidence that NPs induce significant oxidative stress and tissue damage in parotid glands. Multi-strain probiotics provide substantial protection by activating the NRF2/HO-1 pathway and suppressing inflammation, suggesting a promising therapeutic strategy against nanoplastic-induced oral tissue damage. Copyright © 2026 Kozan, Üstündağ and Kara.Frontiers Media SA2026Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/10128https://doi.org/10.3389/fphar.2026.18240521663-9812enFrontiers in Pharmacologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/10128