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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/106932026-10-18T21:13:11Zopenairedrivercom_ebyu_10000col_ebyu_10001

Antioxidant Activity and Phytochemical Profiling of Steam-Distilled Oil of Flaxseed (Linum usitatissimum): Therapeutic Targeting Against Glaucoma, Oxidative Stress, Cholinergic Imbalance, and DiabetesGulcin, İlhamiMutlu, MuzafferBingol, ZeynebeOzden, Eda MehtapMirzaee, ZibaGoren, Ahmet C.Köksal, EkremGC/MSLC-HRMSLinum usitatissimummetabolic disordersradical scavengingAcetylcholinesteraseAntioxidantsDiabetes MellitusHumansLinseed OilOxidative StressPhenolsPhytochemicalsPlant OilsSteamantioxidantphenol derivativephytochemicalvegetable oilchemistrydrug effectdrug therapyhumanwater vaporThis investigation explored the chemical constituents and biological activities of the steam-distilled oil of L. usitatissimum (SDOLU), employing sophisticated techniques including LC-HRMS, GC-MS, and GC-FID. The analysis identified a diverse array of 17 phenolic compounds, with linoleoyl chloride (64.05%) and linoleic acid (10.39%) as the major fatty acid components. The SDOLU demonstrated remarkable antioxidant capacity, effectively neutralizing free radicals in both DPPH• (IC50: 19.80 μg/mL) and ABTS•+ (IC50: 57.75 μg/mL) scavenging assays, alongside robust electron-donating activity in reducing ability tests. Moreover, the SDOLU showed significant inhibition of key enzymes implicated in metabolic and neurodegenerative disorders, including α-amylase (IC50: 531.44 μg/mL), acetylcholinesterase (IC50: 13.23 μg/mL), and carbonic anhydrase II (IC50: 281.02 μg/mL). Collectively, these results highlight the SDOLU as a valuable natural source of multifunctional bioactivities with potential applications in combating oxidative stress and enzyme-related global diseases. Further studies are warranted to validate its therapeutic efficacy and expand its industrial utilization. © 2025 by the authors.Multidisciplinary Digital Publishing Institute (MDPI)2025Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/10693https://doi.org/10.3390/molecules301633841420-3049enMoleculesinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/10693