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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/108432026-10-18T21:13:14Zopenairedrivercom_ebyu_10000col_ebyu_10001

In Vitro, Anti-Colon Cancer Activity of Green Dumbbell-Shaped Rhododendron luteum-Based Carbon DotsDurmaz, AlperMizan Kahyaoğlu, İbrahimCan Aytar, ErdiDanışman Kalındemirtaş, FerdaneSert, EsraErol-Bozkurt, AyşeKarakuş, SelcanAnticancerGreen nanostructureHCT116 colon cancer cellMCF-7 breast cancerRhododendron luteumAnalysisCarbonCellsDiseasesDotsInfrared SpectroscopyTransmission Electron MicroscopyVibrationAntineoplastic Agents, PhytogenicApoptosisCell Line, TumorCell ProliferationCell SurvivalColonic NeoplasmsDrug Screening Assays, AntitumorHumansQuantum DotsRhododendronAtomic emission spectroscopyCell deathLung cancerNear infrared spectroscopyNuclear magnetic resonance spectroscopyantineoplastic agentquantum dotColorectal cancer is the second most common cause of cancer-related deaths worldwide and the third most common cancer overall. In this study, we investigate the anti-colon cancer potential of phytochemically, and thermally synthesised novel green carbon dots based on Rhododendron luteum (RL-CDs). A new synthesis method was used to produce carbon dots obtained from the Rhododendron luteum (RL) plant in an environmentally friendly manner. The green RL were characterized using Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, transmission electron microscopy (TEM), and artificial intelligence (AI)-based TEM analysis. The FTIR spectrum showed peaks corresponding to the hydroxyl (−OH) vibration of polyphenols at 3500 cm−1, the C=O vibration of cellulose derivatives at 1728 cm−1, and the C−O stretching of primary alcohol at 1041 cm−1. Two UV absorption peaks at roughly 253 nm (UV−C range), and 320 nm (UV−B range) were observed. The size of the green RL was measured to be less than 50 nm, and its morphology was characterized as dumbbell-shaped through TEM analysis. In-vitro studies were performed with HCT116 colon cancer, MCF-7 breast cancer, and normal HUVEC cells. The results demonstrated that the RL-CDs exhibited selective cytotoxic activity against HCT116 colon cancer cells. The results show that the RL extract stimulates cancer cell death by decreasing the CD44/24 ratio, and increasing apoptotic activity. These observations suggest that green RL-CDs could be an effective anticancer agent in colon cancer therapy, investigating their potential in this direction could be a promising way for future research. © 2025 The Authors. ChemistryOpen published by Wiley-VCH GmbH.John Wiley and Sons Inc2025Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/10843https://doi.org/10.1002/open.2024003032191-1363enChemistryOpeninfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/10843