2026-10-19T01:04:14Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/109382026-10-18T21:14:17Zopenairedrivercom_ebyu_10000col_ebyu_10001Comprehensive Analysis of Hedysarum nitidum: Identification Phytochemical Contents, Antioxidant Potential, Antimicrobial Properties, Cytotoxic Effects, and Enzyme Inhibition ActivitiesBaltürk, NurayBadem, MerveKanbolat, SeydaYıldırım, SercanŞen, Tuğba MazlumBozdal, GözdeYalçın, Can ÖzgürKandemir, AliKorkmaz, NuriyeKaraoglu, Sengul AlpayAliyazicioglu, Rezzananticholinesterase | antidiabetic | antityrosinase | gas chromatography (GC)/mass spectroscophy (MS) | Hedysarum | high-performance liquid chromatography (HPLC)alpha-Amylasesalpha-GlucosidasesAnti-Bacterial AgentsAnti-Infective AgentsAntineoplastic Agents, PhytogenicAntioxidantsBiphenyl CompoundsCell ProliferationCell SurvivalCholinesterasesDose-Response Relationship, DrugDrug Screening Assays, AntitumorEnzyme InhibitorsHumansMicrobial Sensitivity TestsMonophenol MonooxygenasePhytochemicalsPlant ExtractsStructure-Activity Relationship2 hydroxycinnamic acid4 hydroxybenzoic acidacarboseacetylcholinesterasealcohol derivativealdehydealkaloidalpha cadinolalpha glucosidasealpha terpineoleHedysarum species play a crucial agronomic role due to their high feed quality and ability to enhance soil fertility through nitrogen fixation. Traditionally, they have been used in medicine to boost energy, support the immune system, and benefit the peripheral nervous system. However, the biological activity and phytochemical composition of Hedysarum nitidum remain unexplored. This study aimed to evaluate the bioactivity of methanol (HNM) and water (HNW) extracts from the aerial parts of H. nitidum. Antioxidant (total polyphenol and flavonoid contents, 2,2-diphenyl-2-picrylhydrazyl hydrate [DPPH], ferric-reducing antioxidant power [FRAP], Cu (II) ion reducing antioxidant capacity [CUPRAC]), antimicrobial, cytotoxic, and enzyme inhibitory (tyrosinase, cholinesterase, α-glucosidase, α-amylase) activities were assessed. Phytochemical and essential oil compositions were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography–mass spectroscophy (GC–MS). HPLC identified p-hydroxybenzoic acid, caffeic acid, and 2-hydroxycinnamic acid, whereas GC–MS detected n-octanal, hexahydrofarnesyl acetone, humulene epoxide-II, α-terpineol, and eicosane. HNW and HNM exhibited strong antioxidant activity and enzyme inhibition, suggesting potential applications in hyperpigmentation, diabetes, and Alzheimer's treatment. HNM showed selective antibacterial activity against Gram-positive bacteria, whereas neither extract exhibited cytotoxicity against A549 cells. These findings highlight H. nitidum as a promising candidate for therapeutic applications. © 2025 Wiley-VHCA AG, Zurich, Switzerland.John Wiley and Sons Inc2025Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/10938https://doi.org/10.1002/cbdv.2025009921612-1872enChemistry and Biodiversityinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/10938