2026-10-19T00:34:15Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/113232026-10-18T21:13:14Zopenairedrivercom_ebyu_10000col_ebyu_10001An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluidsAydin, SuleymanEmre, ElifUgur, KaderAydin, Mustafa AtaSahin, İbrahimCinar, VedatAkbulut, TanerBiological fluids, ELISA, antibody, antigenenzyme-linked immunosorbent assayBody FluidsHumansPeptidesProteinsantigenpeptides and proteinspeptideproteinantigen antibody reactionbody fluidcolorconjugatedrug therapyELISA kitenzyme linked immunosorbent assayenzyme substratehumanmetaboliteprotocolreviewchemistryimmunologymetabolismproceduresThe enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field. © The Author(s) 2025.SAGE Publications Ltd2025Review Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/11323https://doi.org/10.1177/030006052513159130300-0605enJournal of International Medical Researchinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/reviewhttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/11323