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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/114252026-10-18T21:13:16Zopenairedrivercom_ebyu_10000col_ebyu_10001

Hepatoprotective effects of resveratrol on α-amanitin-induced liver toxicity in ratsGezer, ArzuUstundag, HilalMendil, Ali SefaBedir, GurselDuysak, LaleHepatotoxicityInflammationOxidative stressResveratrolα-AmanitinAlpha-AmanitinAnimalsAntioxidantsApoptosisChemical and Drug Induced Liver InjuryLiverMaleProtective AgentsRatsRats, Sprague-DawleyStilbenesalanine aminotransferasealpha amanitinaspartate aminotransferasecatalaseglutathione peroxidaseglutathione reductaseimmunoglobulin enhancer binding proteinmalonaldehydemyeloperoxidasenitric oxide synthasereduced nicotinamide adenine dinucleotide phosphatesevofluranethiopentalantioxidantObjective: The hepatoprotective effects of resveratrol against α-Amanitin (α-AMA)-induced liver toxicity were investigated in an experimental rat model, focusing on oxidative stress, inflammation, apoptosis, and liver function. Methods: Thirty-two male Sprague-Dawley rats were divided into four groups (n = 8 per group): Control, resveratrol, α-AMA, and resveratrol+α-AMA. The resveratrol group received 20 mg/kg resveratrol orally for 7 days. The α-AMA group received 3 mg/kg α-AMA intraperitoneally on the 8th day. The resveratrol+α-AMA group received 20 mg/kg resveratrol orally (7 days) followed by 3 mg/kg α-AMA intraperitoneally on the 8th day. Liver tissues and blood samples were collected 48 h after α-amanitin administration for histopathological, immunohistochemical (NFkB, LC3B), and biochemical analyses (GSH, MDA, CAT, GPx, MPO, NOS, AST, ALT). Results: α-AMA significantly increased AST and ALT levels, oxidative stress marker (MDA), and inflammatory marker (MPO), while reducing antioxidant levels (GSH, CAT, GPx) and NOS concentration (P < 0.001 for all parameters). Histopathological analysis showed severe liver damage with increased NFkB and LC3B expression. resveratrol treatment significantly reduced AST and ALT levels (P < 0.01 for both parameters), decreased MDA and MPO levels, and increased NOS concentration, GSH, CAT, and GPx levels (P < 0.05 for all parameters). Reduced NFkB and LC3B expression in the resveratrol+α-AMA group and showed histopathological improvements. Conclusion: Resveratrol demonstrated substantial hepatoprotective effects against α-AMA induced liver toxicity by reducing oxidative stress, inflammation, and apoptosis, and improving liver function. These findings suggest that resveratrol could be a potential therapeutic agent for treating liver damage caused by potent hepatotoxins like α-AMA. © 2024 Elsevier LtdElsevier Ltd2024Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/11425https://doi.org/10.1016/j.toxicon.2024.1078550041-0101enToxiconinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/11425