2026-10-19T00:02:51Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/115372026-10-18T21:13:22Zopenairedrivercom_ebyu_10000col_ebyu_10001Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathwayKoc, KubraOzek, Nihal SimsekAysin, FerhundeDemir, OzlemYilmaz, AsliYilmaz, MehmetGeyikoglu, FatimeErol, Huseyin SerkanACE activityHispidulinlung injury with ARDSAnimalsCaspase 3FlavonesInterleukin-8LungMitogen-Activated Protein KinasesOleic AcidRatsRespiratory Distress SyndromeTumor Necrosis Factor-alphadipeptidyl carboxypeptidaseglutathioneinterleukin 8mitogen activated protein kinasemyeloperoxidasesuperoxide dismutasetumor necrosis factorflavone derivativeapoptosiscellenzymeenzyme activityfatty acidgene expressioninhibitioninjuryorganic compoundThis study investigates the protective role of Hispidulin on acute respiratory distress syndrome (ARDS) in rats. Rats were divided into three groups: control, ARDS, ARDS+ Hispidulin. The ARDS models were established by injecting rats with oleic acid. Hispidulin (100 mg/kg) was injected i.p. an hour before ARDS. Myeloperoxidase (MPO), Interleukin-8 (IL-8), Mitogen-activated protein kinases (MAPK), Lipid Peroxidation (LPO), Superoxide Dismutase (SOD), Glutathione (GSH), and Angiotensin-converting enzyme (ACE) were determined by ELISA. Tumor necrosis factor-alpha (TNF-α) expression was described by RT-qPCR. Caspase-3 immunostaining was performed to evaluate apoptosis. Compared with the model group, a significant decrease was observed in the MPO, IL-8, MAPK, ACE, LPO levels, and TNF-α expression in the ARDS+ Hispidulin group. Moreover, reduced caspase-3 immunoreactivity and activity of ACE were detected in the Hispidulin+ARDS group. The protective effect of Hispidulin treatment may act through inhibition of the ACE activity and then regulation of inflammatory cytokine level and alteration of apoptosis. © 2023 Informa UK Limited, trading as Taylor & Francis Group.Taylor and Francis Ltd.2024Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/11537https://doi.org/10.1080/09603123.2023.21660230960-3123enInternational Journal of Environmental Health Researchinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/11537