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Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin HarmKuzucu, MehmetDeinococcus radioduransdeinoxanthinmelanogenesisUV-induced damageascorbic acidcarotenoidsuperoxide dismutasexanthophyllzeaxanthinArticlecarbon nuclear magnetic resonancecell culturecell cycle progressioncell growthcell migrationcell proliferationcell viabilitychemical compositioncytotoxicity testelemental analysisflow cytometryHFF-1 cell linehumanhuman cellhydrolysisIC50immunohistochemistrylipid peroxidationMTT assaynonhumanThis research delved into the protective capacities of deinoxanthin, a carotenoid present in Deinococcus radiodurans, against UVA- and UVB-mediated skin damage using human fibroblast foreskin cells (HFF-1). Using the MTT assay, HFF-1 cells treated with 10 µM DNX displayed 20% and 31.7% higher viability than the positive (Vitamin C-treated) and negative (DNX-untreated) control groups, respectively, upon 100 mJ/cm2 UVB exposure. At 24 J/cm2 UVA, 20 µM DNX-treated cells showed 80.6% viability, exceeding the positive and negative control groups by 28.6% and 33.6%, respectively. Flow cytometry analysis revealed that cells treated with DNX and exposed to 24 J/cm2 UVA exhibited a 69.32% reduction in apoptotic processes compared to untreated cells. Similarly, when exposed to 100 mJ/cm2 UVB, DNX-treated cells demonstrated a 72.35% decrease in apoptotic processes relative to their untreated counterparts. DNX also displayed dose-dependent inhibition on tyrosinase activity. The study emphasized DNX’s antioxidative capacity, evident in its modulation of superoxide dismutase activity and measurements of Malondialdehyde and intracellular reactive oxygen species levels. DNX-treated cells exhibited higher hydroxyproline levels, suggesting healthier collagen production. Additionally, the wound-healing assay method confirmed an accelerated healing rate in DNX-treated cells. Conclusively, DNX offers significant protection against UV-induced skin damage, emphasizing its potential for skincare and therapeutics. © 2023 by the author.Multidisciplinary Digital Publishing Institute (MDPI)2023Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/11613https://doi.org/10.3390/cimb451005281467-3037enCurrent Issues in Molecular Biologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/11613