2026-10-19T01:06:32Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/117162026-10-18T21:13:19Zopenairedrivercom_ebyu_10000col_ebyu_10001The association of miR-204 and mir-483 5p expression with clinicopathological features of Wilms tumor: Could this provide foresight?Calli, Aylin OrgenIssin, GizemYilmaz, IsmailInce, DilekTural, ErsinGuzelis, IsmailCecen, Refik EmreOlgun, Hatice NurGokcay, DenizOzer, Erdenermicro-RNAmiR-204miR-483-5pWilms tumorCell ProliferationGene Expression Regulation, NeoplasticHumansKidney NeoplasmsMicroRNAsNeoplasm Recurrence, LocalUp-Regulationbiological markermicroRNAmicroRNA 204microRNA 483 5punclassified drugMIRN204 microRNA, humanMIRN483 microRNA, humanadolescentadultArticlecancer stagingcancer survivalchildcontrolled studydown regulationembryoembryo developmentepigeneticsepithelial mesenchymal transitionBackground: Wilms tumor is the most common cancer of the kidney that occurs during childhood, and histologically, it mimics renal embryogenesis. With the development and improvement of up-to-date treatment protocols, the survival rates of Wilms tumor have increased. However, metastases or local relapses are still observed in 15% of patients. The search for reliable biomarkers to identify at-risk patients is ongoing to predict the variability in treatment success. Currently, the evaluation of clinical, histopathological and genetic features are common diagnostic methods; however, epigenetic features can be examined with microRNA expression analyses and might allow us to comment on the behavior of the tumor and treatment response. Methods: In this study, we aimed to evaluate the relationship between microRNA-204 and microRNA-483-5p expression with clinicopathological data and the effect on Wilms tumor survival. For this purpose, the expression levels of RNU6B, microRNA-204 and microRNA-483-5p were evaluated in tumor and normal tissue by qreal time-polymerase chain reaction. We also investigated the relationship between microRNA expression levels with the clinicopathological and histological features of Wilms tumor. Results and conclusion: The results of our study indicate that the relative expression levels of microRNA-204 in Wilms tumor tissues were significantly lower than that in adjacent normal tissues. By contrast, tumor tissue had a higher microRNA-483-5p expression than the corresponding normal tissues. A statistically significant difference between microRNA-204 expression level with age and the presence of anaplasia was observed. The upregulation of microRNA-483-5p was found to have a significant correlation with patients after preoperative chemotherapy and complete tumor necrosis. Taken together, our data suggest that microRNA-204 could play a critical role as a tumor suppressor, whereas microRNA-483-5p acts as an oncogene in Wilms tumor progression. More importantly, microRNA-204 might be a novel predictive biomarker for anaplastic histology and could be useful for developing therapeutic interventions targeting this marker. © The Author(s) 2023. Published by Oxford University Press. All rights reserved.Oxford University Press2023Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/11716https://doi.org/10.1093/jjco/hyad1130368-2811enJapanese Journal of Clinical Oncologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/11716