2026-10-19T04:52:06Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/request

oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/117652026-10-18T20:26:45Zopenairedrivercom_ebyu_10000col_ebyu_10001

In vivo study of the utility of selective intra-arterial injection of thiopental for neuroprotection in reversible cerebral ischemiaGökten, MuratÖcal, OsmanSezer, CanZırh, SelimMuftuoglu, SevdaÖcal, ElifBilginer, BurcakArat, Anılcerebral ischemiaDegradable starch microsphereintra-arterialneuroprotectionthiopentalAnimalsBrain IschemiaCerebral InfarctionHumansInjections, Intra-ArterialIschemiaNeuroprotective AgentsRabbitsCellsCytologyMicrospheresNeuronsStarchketamineneuroprotective agentstarch microspherexylazineApoptoticGlial cellsGroup 4Neuronal cellStarch microspheresanimal cellanimal experimentanimal modelBackground: Neuroprotective agents are needed to reduce cerebral damage during surgical or neurointerventional procedures including stroke patients. Purpose: To evaluate if thiopental can be used as a neuroprotective agent when injected intra-arterially in a transient ischemia model. Material and Methods: In total, 24 rabbits were studied as four groups of six animals. Group 1 served as the control group. In group 2, transient ischemia was obtained by intracarotid administration of degradable starch microspheres (DSM). Group 3 was administered thiopental intra-arterially via the carotid artery. Group 4 (experimental group) received both thiopental and DSM intra-arterially. DSM and thiopental were administered through a microcatheter placed into the common carotid artery via the central ear artery access. After sacrifice, apoptotic cells in the cerebral tissues of the animals were evaluated in H&E and TUNEL stained slides. Results: There was a significant increase in the number of apoptotic glial or neuronal cells in group 2 compared to the control group and group 3. The mean number of both the apoptotic neuronal cells (6.8 ± 2.1 vs. 2.5 ± 1.3, P < 0.001) and the apoptotic glial cells (9.4 ± 3.1 vs. 4.6 ± 1.6, P < 0.001) were higher in group 2 compared to group 4. In addition, a higher level of neurological improvement was observed in group 4 compared to group 2 based on neurological assessment score. Conclusion: The intra-arterial administration of thiopental has a protective effect on both glial and neuronal cells during temporary cerebral ischemia in low doses. © The Foundation Acta Radiologica 2023.SAGE Publications Inc.2024Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/11765https://doi.org/10.1177/028418512312065030284-1851enActa Radiologicainfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/11765