2026-10-19T01:10:12Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/121192026-10-18T21:13:20Zopenairedrivercom_ebyu_10000col_ebyu_10001Protective effect of astaxanthin and metformin in the liver of rats in which the polycystic ovary syndrome model was formed by giving letrozoleTaştan, Tuǧba BalAkaras, NurhanDemir, ÖzlemUgan, Rüstem AnilAstaxanthineLiverMetforminOxidative stressPolycystic ovary syndromeRatastaxanthincaspase 3eosinhematoxylinimmunoglobulin enhancer binding proteinletrozolemalonaldehydesuperoxide dismutaseadultanimal cellanimal experimentanimal modelanimal tissueantioxidant activityArticlefemalehistologyimmunohistochemistryliver cellliver protectionnonhumanovary polycystic diseaseObjective(s): In this study, the effects of astaxanthin on liver tissue in rats with polycystic ovary syndrome (PCOS) were evaluated. Materials and Methods: Fifty-four Spraque-Dawley rats were divided into 9 groups: Groups: Control, PCOS, PCOS+Metformin (Met), PCOS+ Astaxanthin (ASX)10, PCOS+ASX20, PCOS+ASX40, PCOS+Met+ASX10, PCOS+Met+ASX20, and PCOS+Met+ASX40. PCOS was induced in female rats by oral administration of letrozole (1 mg/kg) for 21 days. Rats were treated with ASX (10 mg/kg), ASX (20 mg/kg), ASX (40 mg/kg), and metformin (20 mg/kg) for 7 days after PCOS induction. At the end of the experiment, malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured in the liver tissue. The liver was stained with hematoxylin/eosin for histological examination. Additionally, NF-kB and caspase 3 were analyzed immunohistochemically. Results: A remarkable abnormality was observed in the biochemical and histological parameters in the liver tissue of the PCOS model rats. Astaxanthin dose-dependently normalized the MDA level. Additionally, astaxanthin showed a protective effect by increasing the SOD level and increasing its antioxidant activities. We observed that administration of astaxanthin in addition to metformin applied in the standard was more effective. Caspase 3 and NF-kB immune positivity was lower in the groups given astaxanthin compared with PCOS. Histologically, it was observed that astaxanthin improved the deteriorated liver morphology in the letrozole-induced PCOS group. Conclusion: According to our results, it was observed that astaxanthin had antioxidant, anti-inflammatory and anti-apoptotic effects on PCOS in the treatment groups. Therefore, it was concluded that astaxanthin may have a protective effect against PCOS side effects. © 2023 Mashhad University of Medical Sciences. All rights reserved.Mashhad University of Medical Sciences2023Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/12119https://doi.org/10.22038/ijbms.2023.68032.148722008-3866enIranian Journal of Basic Medical Sciencesinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/12119