2026-10-19T01:08:44Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/122342026-10-18T21:13:19Zopenairedrivercom_ebyu_10000col_ebyu_10001Exploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining ApplicationsBeşer, Burcu MeryemYildirim, BeratBiophysicscell imagingcell stainingcytotoxicityFRETthermodynamic3T3-L1 CellsAdenocarcinomaAnimalsEnergy TransferFluorescent DyesHT29 CellsHumansMiceSerum Albumin, HumanCell cultureFluorescence spectroscopyForster resonance energy transferProbesSodium dodecyl sulfateSulfur compoundsfluorescent dyehuman serum albuminBiological interactionsEnergy cellsEnergy-transferFluorescent probesHuman serum albuminsPropertyPyrazolinesFluorescent dyes are used in biological systems, because they are highly sensitive and selective. In this work, we investigated the fluorescent probe properties of 2-(5-(pyridin-2-yl)-1H-pyrazol-3-yl) phenol (PYDP) in two media [sodium dodecyl sulfate (SDS) and human serum albumin (HSA)]. Energy transfer parameters, photophysical and thermodynamic parameters of probe were determined. We investigated cytotoxicity of PYDP against colorectal adenocarcinoma cell lines (HT-29), breast cancer cell lines (MCF-7) and 3T3-L1 adipocytes (3T3 L1) cells. The cell staining property of PYDP was monitored using a confocal microscope. The results showed that PYDP binds to HSA, bindings are due to electrostatic/ionic interactions, and the binding process is spontaneous. PYDP was found to exhibit negligible cytotoxicity at high concentrations, and confocal microscope images showed that PYDP stained the cytoplasm of MCF-7 cells. © 2023 The Authors. ChemistryOpen published by Wiley-VCH GmbH.John Wiley and Sons Inc2023Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/12234https://doi.org/10.1002/open.2023000922191-1363enChemistryOpeninfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/12234