2026-10-19T00:37:40Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/request

oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/123772026-10-18T21:13:19Zopenairedrivercom_ebyu_10000col_ebyu_10001

Clinicopathologic Features and Prognostic Significance of Immunohistochemistry and In Situ Hybridization Based Molecular Classification in Gastric CarcinomaIssin, GizemSayar, İlyasDemir, FatihBakkaloğlu, İrem GüvendirGamsizkan, MehmetYildiz, ZelihaYilmaz, İsmailÖzmen, Sevilay AkalpÇağatay, Diren VuslatZemheri, Itır EbruDemiriz, MuratGünald, Armağanabnormal e-cadherin expressionabnormal p53 expressionCDH1 mutationEBV associated gastric cancermicrosatellite instabilitymolecular classification of gastric carcinomaB7-H1 AntigenCarcinomaEpstein-Barr Virus InfectionsHerpesvirus 4, HumanHumansImmunohistochemistryIn Situ HybridizationPrognosisStomach NeoplasmsTumor Suppressor Protein p53epidermal growth factor receptor 2programmed death 1 ligand 1protein p53uvomorulinadultageArticlecancer prognosiscancer sizecancer stagingcancer survivalclinical featureepithelial mesenchymal transitionEpstein Barr virusBackground/Aim: Gastric carcinoma (GC) is a highly heterogeneous disease with many subtypes that have different morphologic and molecular characteristics. In the current study, we analyzed immunohistochemical (IHC) and in situ hybridization (ISH) features of GCs and evaluated their association with prognosis and clinicopathological features. Materials and Methods: Three hundred cases analyzed by IHC and ISH for microsatellite stability, p53, e-cadherin, HER2, PD-L1 expression, and Epstein-Barr virus (EBV) status. Cases were classified into five subgroups based on expression profile. The relationships between subgroups, clinicopathological features, and survival were determined. Results: Ten (3.3%) cases were classified as EBV-associated, 45 (15%) as microsatellite instable (MSI), 73 (24.3%) as EBV–/microsatellite-stable (MSS)/epithelial−mesenchymal−transformation (EMT)-like, 75 (25%) as EBV–/MSS/non-EMT-like/p53+, and 97 (32.3%) as EBV–/MSS/non-EMT-like/p53–. The MSI subtype had the best overall survival (OS). In contrast, the EBV–/MSS/EMT-like subtype had the poorest OS. The MSI subtype was also related with old age of the patient and antrum-corpus localized tumors, whereas the EBV–/MSS/EMT-like was associated with young age, larger tumor size, and advanced stage presentation. PD-L1 positivity is highly correlated with MSI and EBV-associated subtypes. Conclusion: Our data demonstrated a link between IHC/ISH characteristics of GC and clinical outcomes. IHC/ISH based molecular classification may be helpful in predicting the survival. © 2023 by Begell House, Inc.Begell House Inc.2023Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/12377https://doi.org/10.1615/jenvironpatholtoxicoloncol.20230476620731-8898enJournal of Environmental Pathology, Toxicology and Oncologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/12377