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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/131482026-10-18T21:13:28Zopenairedrivercom_ebyu_10000col_ebyu_10001

Investigation of the Role of Stimulation and Blockade of 5-HT7 Receptors in Ketamine Anesthesia.Dincer, BusraHalici, ZekaiCadirci, Elif5-HT₇ receptorAnesthesiaKetamineNMDA receptorSerotoninAnalgesicsAnimalsHippocampusMaleMiceMice, Inbred BALB CPrefrontal CortexReceptors, N-Methyl-D-AspartateReceptors, SerotoninSerotonin AntagonistsSerotonin Receptor Agonists3 [2 [2 (4 methyl 1 piperidinyl)ethyl] 1 pyrrolidinesulfonyl]phenolas 19complementary DNAmessenger RNAn methyl dextro aspartic acid receptorn methyl dextro aspartic acid receptor 2An methyl dextro aspartic acid receptor 2Bserotonin 7 receptorserotonin agonistunclassified druganalgesic agentserotonin antagonistserotonin receptoranimal experimentAlthough several pieces of evidence have indicated the ability of the serotonin-7 receptor (5-HTR7) to modulate N-methyl-D-aspartate receptor (NMDAR) activation, the possible impact on ketamine anesthesia has not been examined directly. The purpose of the present study is thus to investigate the possible role of the 5-HTR7 in ketamine anesthesia using a 5-HTR7 agonist and/or antagonist. The influence of a 5-HTR7 agonist/antagonist on ketamine anesthesia for behavioral impact was assessed by testing potential anesthetic parameters. Its functional impact was assessed by mRNA expression with real-time PCR and immunostaining in the hippocampus and prefrontal cortex of mice. Two different doses of ketamine—high and low—were administered to induce anesthesia. In the high-dose ketamine-applied group in particular, the administration of both the 5-HTR7 agonist and antagonist intensified the anesthetic effect of ketamine. The reflection of the change in anesthesia parameters to 5-HTR7 expression was observed as an increase in the hippocampus and a decrease in the prefrontal cortex in the anesthetized groups by stimulation of 5-HTR7. It is noteworthy that the results of NMDAR expressions are parallel to the results of the 5-HTR7 expressions of both the hippocampus and the prefrontal cortex. The 5-HTR7 may play a role in ketamine anesthesia. It may act through NMDAR in ketamine anesthesia, depending on the parallelism between both receptors. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.Humana Press Inc.2021Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/13148https://doi.org/10.1007/s12031-020-01732-30895-8696enJournal of Molecular Neuroscienceinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/13148