2026-10-19T04:52:29Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/137772026-10-18T21:13:30Zopenairedrivercom_ebyu_10000col_ebyu_10001Effect of taxifolin on development of retinopathy in alloxan-induced diabetic ratsAhiskali, IbrahimPinar, Can LokmanKiki, MuratMammadov, RenadOzbek Bilgin, AsliHacimuftuoglu, AhmetCankaya, MuratKeskin Cimen, FerdaAltuner, DurduAlloxandiabetic retinopathyratretina toxicitytaxifolinAnimalsAnti-Inflammatory Agents, Non-SteroidalDiabetes Mellitus, ExperimentalGlutathioneInterleukin-1betaMaleMalondialdehydeQuercetinRats, WistarRetinal Ganglion CellsTumor Necrosis Factor-alphaglucoseinterleukin 1betamalonaldehydereactive oxygen metabolitetumor necrosis factorIL1B protein, humannonsteroid antiinflammatory agentalloxan-induced diabetes mellitusanimal experimentanimal modelanimal tissueArticlebiochemical analysisblindnessPurpose: Diabetic retinopathy (DR) is one of the leading causes of blindness. In DR patients, antioxidant defence is disrupted, and production of reactive oxygen species and pro-inflammatory cytokines such as interleukin 1β (IL-1β) and tumour necrosis factor alpha (TNF-α) increases. Taxifolin has been reported to suppress reactive oxygen species, IL-1β and TNF-α production. The aim of this study is to biochemically and histopathologically examine the protective effect of taxifolin against DR damage induced by alloxan. Materials and methods: Alloxan received rats with a blood glucose level of ≥250 mg/dL were divided into taxifolin-treated (TAX) (n = 6), diabetic control (DC) (n = 6) groups. There were rats received only saline in non-diabetic control (NC) group (n = 6). Taxifolin (50 mg/kg) was orally administered to the TAX group rats. DC and NC rats received the same volume of saline as a solvent. This procedure was repeated once a day for 3 months. At the end of this period, animals were killed by high dose thiopental sodium anaesthesia. Histopathological examinations were then performed on excised rat eyes. Malondialdehyde (MDA), total glutathione (tGSH), IL-1β and TNF-α levels were measured in obtained blood samples. Results: MDA, IL-1β and TNF-α levels were significantly increased in blood samples of DC group rats with hyperglycemia induced by alloxan compared with NC group (p < 0.0001), and decreased in the TAX group compared with the DC group (p < 0.0001). The levels of tGSH were significantly decreased in blood samples of DC group rats compared with NC group (p < 0.0001), and increased in the TAX group compared with the DC group (p < 0.0001). Histopathologically, retinal ganglion cells of the TAX group had a slightly dilated and congested blood vessel, and severe damage was inflicted to the retinal ganglion cell layer of the DC group. Conclusions: Experimental results suggest that taxifolin may be beneficial in the treatment of DR. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.Taylor and Francis Ltd2019Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/13777https://doi.org/10.1080/15569527.2019.15882891556-9527enCutaneous and Ocular Toxicologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/13777