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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/137952026-10-18T20:28:36Zopenairedrivercom_ebyu_10000col_ebyu_10001

The role of protective effect of zingerone in gene regulation against genotoxic effect of 2,4-D isooctylester herbicide on barley speciesBulut, Huseyin24-D IsooctylesterBarley speciesGene expressionGenotoxic effectHerbicidesMiRNAVanillylacetoneHordeum2,4 dichlorophenoxyacetic acidbarleyecotoxicologyfood productiongenotoxicitymutationpesticide residuepollution exposureproteinIt has become increasingly important in the fight against pests that cause more than 50% of annual food production to disappear. Pesticides used against agricultural pests play an important role in increasing the yield. However, pesticides cause various problems such as deterioration of ecological balance, contamination of nutrients, teratogenic, mutagenic and carcinogenic effects in metabolisms, increase of environmental pollution depending on frequency of use. These substances, which have genotoxic effects, can change the structure or gene expression of DNA in a way that cannot be reversed. Different plant extracts and extracts were used differently in different organisms to determine the protective effects of natural antioxidants in order to reduce or prevent the effect of toxins. In our research, the protective effect of vanillylaceton against genotoxic effect caused by 2,4-D isooctylester herbicide was investigated in Aydanhamm, Ince 04 and Tanm 92 barley species. For this, 14.3.3 defense protein gene expression analysis, miRNA 156, miRNA 159 and miRNA 171 expression analyzes were performed. It has been found that the dose of 2,4-D isooctylester herbicide increases gene expression as the stress response in barley species, while vanillylaceton decreases stress level and thus gene expression. In addition, miRNA 156 and miRNA 159 gene regulation showed positive correlation with gene expression and miRNA 171 gene regulation showed negative correlation. Moreover, it was found that 2,4-D isooctylester herbicide showed genotoxic effect at least in Ince 04 and most in Aydanhamm species. Herbicides cause environmental pollution and genotoxic effects in non-target organisms. In the light of the data obtained from our study, vanillylacetone has a protective effect against herbicide stress. © 2020 Parlar Scientific Publications. All rights reserved.Parlar Scientific Publications2020Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/137951018-4619enFresenius Environmental Bulletininfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/13795