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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/147352026-10-18T21:14:24Zopenairedrivercom_ebyu_10000col_ebyu_10001

Effect of Kineret® on ovarian ischemia reperfusion injury in a rat modelNayki, Umit ArslanNayki, CenkCetin, NihalCimen, Ferda KeskinCoban, AbdulkadirMammadov, RenadTas, Ismail HakkıMalkoc, IsmailapoptosisIL-1βischemia/reperfusionkineretovaryratAnimalsAnti-Inflammatory AgentsAntioxidantsCaspase 3Disease Models, AnimalFemaleGene ExpressionGlutathioneInterleukin 1 Receptor Antagonist ProteinInterleukin-1betaMalondialdehydePeroxidaseRatsRats, WistarReperfusion Injurycomplementary DNAinterleukin 1betamalonaldehydemyeloperoxidaserecombinant interleukin 1 receptor blocking agentantiinflammatory agentantioxidantCasp3 protein, ratinterleukin 1 receptor blocking agentAim: To investigate the effect of Kineret® on ischemia reperfusion (IR) injury in rat ovaries. Methods: Rats were divided into four groups: ovarian IR (IRG); 50 mg/kg Kineret® + ovarian IR (KIR-50); 100 mg/kg Kineret® + ovarian IR (KIR-100); and sham operation (SOC). KIR-50 (n = 10) and KIR-100 (n = 10) groups received an intraperitoneal injection of Kineret® at doses of 50 and 100 mg/kg, respectively. IRG and SOC (n = 10) rat groups were given distilled water as solvent using the same method. The results were compared between the groups. Results: In rats in which IR occurred, oxidant parameters, such as malondialdehyde (MDA) and myeloperoxidase (MPO), were increased, the level of proinflammatory interleukin 1 beta (IL-1β) was elevated and total glutathione (tGSH) as an antioxidant was decreased in the ovarian tissues. Administration of Kineret® at a dose of 100 mg/kg inhibited the increase of MDA, MOP and IL-1β and a decrease in tGSH caused by IR more significantly than administration of Kineret® at a dose of 50 mg/kg. In addition, 100 mg/kg Kineret® significantly decreased severe hemorrhage, degeneration and inflammatory signs in the follicular cells, caused by IR. Kineret® at 100 mg/kg markedly ameliorated increased apoptosis in ovarian tissue with IR more significantly than 50 mg/kg kineret. Conclusion: Our findings indicate that Kineret® might be useful in clinical practice for the treatment of damage that may occur as a result of ovarian torsion. © 2016 Japan Society of Obstetrics and GynecologyBlackwell Publishing2016Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/14735https://doi.org/10.1111/jog.130951341-8076enJournal of Obstetrics and Gynaecology Researchinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/14735