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oai:ebyu-rdlopen.rawdatalibrary.net:ebyu/149822026-10-18T21:14:26Zopenairedrivercom_ebyu_10000col_ebyu_10001

The effect of melatonin on oxidative stress and apoptosis in experimental diabetes mellitus-related ovarian injuryNayki, UmitOnk, DidemBalci, GurhanNayki, CenkOnk, AlperÇankaya, MuratTaskin Kafa, Ayse HumeyraKuzucu, MehmetApoptosisdiabetesNFkB pathwayovaryoxidative stressratAnimalsCaspase 3Diabetes Mellitus, ExperimentalFemaleMelatoninNF-kappa BRatsRats, Sprague-Dawleycatalaseimmunoglobulin enhancer binding proteinmalonaldehydesuperoxide dismutaseanimal experimentanimal modelanimal tissueantioxidant activityArticlecontrolled studydiabetes mellitusdrug effectenzyme activityexperimental diabetes mellitushistopathologyimmunohistochemistryWe aimed to evaluate the effect of melatonin on oxidative stress and ovarian injury in rats. Twenty-four Sprague-Dawley albino rats were divided into three groups: Group 1 as nondiabetic healthy controls (n = 8), group 2 as nontreated diabetic rats (n = 8) and group 3 as melatonin-treated diabetic rats (n = 8). After overt diabetes was produced by intraperitoneal injection of streptozosin, 20 mg/kg/day of melatonin was given intraperitoneally to group 3 for a week. NF-kB and caspase-3 immunoexpressions, lipid peroxidation, the activities of antioxidative enzymes, total oxidant capacity and total antioxidant capacity were assessed. Immunoexpressions of NF-kB and caspase-3 were significantly lower in group 3 than group 2. There was a significant decrease in superoxide dismutase activity in group 2 than group 1 and a significant increase in group 3 compared with group 2. We observed a nonsignificant decrease in catalase activity between group 1 and group 2 and a nonsignificant increase between group 2 and group 3. There was a nonsignificant increase in the plasma level of total oxidant status in group 2 than group 1, but a significant decrease was observed in group 3 compared to group 2. Total antioxidant status was significantly lower in group 2 compared with group 1 and group 3. In conclusion, melatonin ameliorates the negative effects of oxidative stress on DM-related ovarian injury. © 2015 Taylor & Francis.Taylor and Francis Ltd2016Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/14982https://doi.org/10.3109/09513590.2015.11268190951-3590enGynecological Endocrinologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/14982