2026-10-19T01:07:57Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/156942026-10-18T20:39:13Zopenairedrivercom_ebyu_10000col_ebyu_10001The inhibitory effects of L-adrenaline on lactoperoxidase enzyme purified from bovine milkŞisecioglu, MeldaGülçin, IlhamiÇankaya, MuratOzdemir, HasanEnzyme purificationInhibitionL-AdrenalineLactoperoxidaseBovinaeBiologyColloidsDextranGel permeation chromatographyIon exchangeMammalsPurificationAmberlite CG-50Bovine milkChromogenic substrateGel-filtration chromatographyInhibition constantsInhibitory concentrationInhibitory effectLiving organismsPhysiological processSDS-PAGESephadexSingle bandSpecific activityEnzyme inhibitionL-Adrenaline belongs to a group of compounds known as catecholamines, which play an important role in the regulation of the physiological process in living organisms. In the present study, the inhibitory effect of L-adrenaline on lactoperoxidase was examined. Lactoperoxidase (E.C.1.11.1.7) was purified from bovine milk with three consecutive steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange, and Sephadex G-100 gel filtration chromatography. Lactoperoxidase was purified with a yield of 42.18%, a specific activity of 30.33 EU/mg proteins, and 20.77 purification fold. Enzyme purity was determined with SDS-PAGE, where a single band was observed. The Rz (A 412/A280) value for lactoperoxidase was 0.9. The effect of L-adrenaline on lactoperoxidase was determined using ABTS as a chromogenic substrate. The half maximal inhibitory concentration (IC50) value and an inhibition constant (Ki) values for L-adrenaline were 34.5 and 2.26 μM, respectively. L-Adrenaline was found to be a non-competitive inhibitor. Copyright © Taylor & Francis Group, LLC.TAYLOR & FRANCIS INC2012Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/15694https://doi.org/10.1080/10942912.2010.5119241532-2386enInternational Journal of Food Propertiesinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/15694