2026-10-19T00:37:40Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/159252026-10-18T20:30:46Zopenairedrivercom_ebyu_10000col_ebyu_10001Effects of some metal ions on trout liver glucose 6-phosphate DehydrogenaseCankaya, M.Sisecioglu, M.Ciftci, M.Ozdemir, H.G6PD enzymeInhibitionMetal ionscadmiumcopperglucose 6 phosphataseironleadmercuryzincaffinity chromatographyanimal tissuearticlecompetitive inhibitionconcentration (parameters)controlled studyenzyme activityenzyme inhibitiongel chromatographyin vitro studynonhumanpolyacrylamide gel electrophoresisrainbow troutInformation on the mechanism of metal ion inhibition of Glucose 6-phosphate dehydrogenase (G6PD) is limited. The purpose of the present study was to clarify in vitro effect of Hg +2, Cd +2, Pb +2, Fe +2, Cu +2 and Zn +2ions on the purified glucose 6-phosphate dehydrogenase (G6PD). This enzyme was purified from rainbow trout liver (Oncorhynchus mykiss) by 2',5'-ADP sepharose 4B affinity gel chromatography. G6PD was purified 1010-fold with a yield of 41.72%. Purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) method and a single band was observed. All of the metal ions caused inhibition of the enzyme activity. Of the metal ions tested, Fe +2 exhibited much higher inhibitory effect at lower concentrations, so it was evidently a more potent inhibitor than the others. Hg +2, Cd +2, Pb +2, Cu +2 and Zn +2 displayed noncompetitive type of inhibition mechanism only Fe +2 exhibited competitive type inhibition. IC 50 value of Fe +2, Pb +2, Hg +2, Cu +2, Zn +2 and Cd +2 were 0.39, 0.78, 0.87, 1.19, 1.97, 2.16 and the Ki constants 0.197±0.049, 0.213±0.018, 0.542±0.029, 1.721±0.77, 2.034±0.53 and 2.770±1.21, respectively. © 2011 Academic Journals Inc.2011Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/15925https://doi.org/10.3923/rjet.2011.385.3911819-3420enResearch Journal of Environmental Toxicologyinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/15925