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Effects of nicotine and vitamin E on glucose 6-phosphate dehydrogenase activity in some rat tissues in vivo and in vitroGumustekin, KenanCiftci, MehmetCoban, AbdulkadirAltikat, SayitAktas, OmerGul, MustafaTimur, HandanDane, SenolGlucose 6-phosphate dehydrogenaseInactivationInhibitionNicotineRat tissuesVitamin EAnimalsEnzyme InhibitorsGlucosephosphate DehydrogenaseOrgan SpecificityRatsRats, Sprague-DawleyAnimaliaalpha tocopherolglucose 6 phosphate dehydrogenaseanimal experimentanimal tissuearticlebrain homogenatecontrolled studycorrelation functiondrug mechanismenzyme activationenzyme activityenzyme inactivationenzyme inhibitionheartin vitro studyin vivo studykidney homogenateEffects of nicotine, and nicotine + vitamin E on glucose 6-phosphate dehydrogenase (G-6PD) activity in rat muscle, heart, lungs, testicle, kidney, stomach, brain and liver were investigated in vivo and in vitro on partially purified homogenates. Supplementation period was 3 weeks (n = 8 rats per group): nicotine [0.5 mg/kg/day, intraperitoneal (ip)]; nicotine + vitamin E [75 mg/kg/day, intragastric (ig)]; and control group (receiving only vehicle). The results showed that nicotine (0.5 mg/kg, ip) inhibited G-6PD activity in the lungs, testicle, kidney, stomach and brain by 12.5% (p < 0.001), 48% (p < 0.001), 20.8% (p < 0.001), 13% (p < 0.001) and 23.35% (p < 0.001) respectively, and nicotine had no effects on the muscle, heart and liver G6PD activity. Also, nicotine + vitamin E inhibited G-6PD activity in the testicle, brain, and liver by 32.5% (p < 0.001), 21.5% (p < 0.001), and 16.5% (p < 0.001) respectively, and nicotine + vitamin E activated the muscle, and stomach G-6PD activity by 36% (p < 0.05), and 20% (p < 0.001) respectively. In addition, nicotine + vitamin E did not have any effects on the heart, lungs, and kidney G-6PD activity. In addition, in vitro studies were also carried out to elucidate the effects of nicotine and vitamin E on G-6PD activity, which correlated well with in vivo experimental results in lungs, testicles, kidney, stomach, brain and liver tissues. These results show that vitamin E administration generally restores the inactivation of G-6PD activity due to nicotine administration in various rat tissues in vivo, and also in vitro. © 2005 Taylor & Francis.2005Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/16171https://doi.org/10.1080/147563605002773841475-6374enJournal of Enzyme Inhibition and Medicinal Chemistryinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/16171