2026-10-19T04:50:22Zhttps://ebyu-rdlopen.rawdatalibrary.net/oai/requestoai:ebyu-rdlopen.rawdatalibrary.net:ebyu/161832026-10-18T20:30:59Zopenairedrivercom_ebyu_10000col_ebyu_10001Investigation of the inhibition effects of some antibiotics on human erythrocyte carbonic anhydrase isozymesCoban, T.A.Nalbantoglu, B.Cil, M.Y.Ozdemir, H.Kufrevioglu, O.I.AntibioticsCarbonic anhydrase isozymesInhibitionantibiotic agentcarbonate dehydratasecefazolincefoperazonecefradinechloramphenicol derivativesulbactamacidificationaffinity chromatographyantimicrobial activityarticlebasolateral membraneblood samplingbrush borderdrug effectenzyme activityerythrocyte membranehumanhuman cellkidney tubule absorptionmembrane bindingIn this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chromatography. Inhibition effects of four different antibiotics on CA isozymes were determined by using the CO2-Hydratase method. I50 values of antibiotics were obtained by drawing % Activity [Actibiotic] graphs. The obtained I50 values of cefazolin sodium, cephradine, sulbactan/cefoperazone and chloramphenicol sodium succinate were 9, 16, 19 and 48 mM on CA-I and 6, 10, 15 and 17 mM on CA-II, respectively. Antibiotics dosages should be taken into consideration because of the inhibition effect on CA which pH regulatory enzyme in body.1998Articlehttps://rdlopen.rawdatalibrary.net/r/ebyu/161831300-0144enTurkish Journal of Medical Sciencesinfo:eu-repo/semantics/openAccessinfo:eu-repo/semantics/articlehttps://ebyu-rdlopen.rawdatalibrary.net/tr/items/ebyu/16183