IN VITRO ASSESSMENT OF ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY, AND IDENTIFICATION OF PHENOLIC PROFILE OF GYPSOPHILA LEPIDIOIDES BOISS. EXTRACTS FROM TURKEY

The aim of this study was to evaluate the antioxidant and anti-proliferative activities of Gypsophila lepidiodies Boiss. together with investigation of phenolic content. Antioxidant activity of water and methanol extracts were evaluated by four different methods namely, DPPH and ABTS radical scavenging activity, cupric ion reducing antioxidant capacity (CUPRAC), and Fe2+ chelating assay. Antiproliferative activities of the extracts were assessed against various human cancer cell lines namely, breast adenocarcinoma (MCF-7), colorectal adenocarcinoma (HT-29) and hepatocellular carcinoma (HepG2) cells. HPLC analysis indicated the presence of eight phenolic compounds in eighteen phenolic standards scanned. Antioxidant activity results showed the superiority of methanol extract to water extract according to DPPH, CUPRAC and Fe2+ chelating tests, while the water extract displayed more antioxidant activity by ABTS assay. Antiproliferative activity results demostrated that methanol extract displayed about 2.5-fold, 3.3-fold and 6-fold more activity against MCF-7 cells, HepG2 cells and HT-29 cells, respectively. However, both water and methanol extracts were observed to display moderate biological activity compared to positive controls. The obtained data suggest that Gypsophila lepidioides may be evaluated as a promising source for food and nutraceutical industries due to its good antioxidant and moderate antiproliferative potentials together with its rich phytochemical profile.