Investigation of Bromelain, Inulin and Saccharomyces cerevisiae Effects Against Escherichia coli Infection Developed in Fibroblast Wound Model; [Bromelain, İnulin ve Saccharomyces cerevisiae’nin Fibroblast Yara Modelinde Gelişen Escherichia coli Enfeksiyonuna Karşı Etkilerinin Araştırılması]

Aim: Wound infections, especially in today’s world where antibiotic resistance is increasing, pose a serious health problem. Escherichia coli complicates treatment by forming a biofilm. In this study, the individual and combined effects of bromelain, inulin, and Saccharomyces cerevisiae on an Escherichia coli infection model created in fibroblast cells were investigated. Methods: The antibacterial activity of bromelain, inulin, and Saccharomyces cerevisiae was evaluated using the disk diffusion method; minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) analyses were conducted. Biofilm inhibitory activity was evaluated using the crystal violet staining method. In vitro wound model has been created using human fibroblast cells. Bromelain, inulin, and Saccharomyces cerevisiae agents were applied to the cells infected with Escherichia coli. Cell viability was determined by the MTT test and analyzed through measurements of total oxidant status (TOS) and total antioxidant capacity (TAC). To evaluate the inflammatory response, IL-1β and IL-10 levels were quantitatively measured using the ELISA method. Results: According to disk diffusion, MIC/MBC, FIC, and antibiofilm analyses, it has been determined that the triple combination (Bromelain + Inulin + Saccharomyces cerevisiae) provides the highest antibacterial and synergistic effect. In the antibiofilm tests, bromelain showed the strongest inhibitory effect (58.3%), but the effect of the triple combination was more limited. In the MTT analysis, cell viability increased in the treatment groups, reaching 112% especially in the triple combination. While TOS levels increased with infection, they significantly decreased after treatment, whereas TAC levels increased. In ELISA analyses, the IL-1β level significantly increased with infection, but decreased with treatment. IL-10 levels, on the other hand, significantly increased, especially in the triple combination, demonstrating an anti-inflammatory response. Conclusions: The combination of bromelain, inulin, and Saccharomyces cerevisiae has shown antimicrobial, antioxidant, and anti-inflammatory activity against Escherichia coli infection, thereby supporting wound healing. © 2025, Selcuk University. All rights reserved.