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Exploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining Applications

ArticleAçık Erişim

Özet

Fluorescent dyes are used in biological systems, because they are highly sensitive and selective. In this work, we investigated the fluorescent probe properties of 2-(5-(pyridin-2-yl)-1H-pyrazol-3-yl) phenol (PYDP) in two media [sodium dodecyl sulfate (SDS) and human serum albumin (HSA)]. Energy transfer parameters, photophysical and thermodynamic parameters of probe were determined. We investigated cytotoxicity of PYDP against colorectal adenocarcinoma cell lines (HT-29), breast cancer cell lines (MCF-7) and 3T3-L1 adipocytes (3T3 L1) cells. The cell staining property of PYDP was monitored using a confocal microscope. The results showed that PYDP binds to HSA, bindings are due to electrostatic/ionic interactions, and the binding process is spontaneous. PYDP was found to exhibit negligible cytotoxicity at high concentrations, and confocal microscope images showed that PYDP stained the cytoplasm of MCF-7 cells. © 2023 The Authors. ChemistryOpen published by Wiley-VCH GmbH.

Anahtar Kelimeler

Biophysicscell imagingcell stainingcytotoxicityFRETthermodynamic3T3-L1 CellsAdenocarcinomaAnimalsEnergy TransferFluorescent DyesHT29 CellsHumansMiceSerum Albumin, HumanCell cultureFluorescence spectroscopyForster resonance energy transferProbesSodium dodecyl sulfateSulfur compoundsfluorescent dyehuman serum albuminBiological interactionsEnergy cellsEnergy-transferFluorescent probesHuman serum albuminsPropertyPyrazolines
RDL Kalıcı Bağlantı (PID)
https://rdlopen.rawdatalibrary.net/r/ebyu/12234Resmi atıflarda kullanılan kalıcı adres
DOI
https://doi.org/10.1002/open.202300092Yayıncı resmi DOI adresi
Tam Kayıt
AlanDeğerDil
dc.contributor.authorBeşer, Burcu Meryem
dc.contributor.authorYildirim, Berat
dc.date.accessioned2026-10-18T20:27:09.482372580Z
dc.date.available2026-10-18T20:27:09.482372580Z
dc.date.issued2023
dc.description.abstractFluorescent dyes are used in biological systems, because they are highly sensitive and selective. In this work, we investigated the fluorescent probe properties of 2-(5-(pyridin-2-yl)-1H-pyrazol-3-yl) phenol (PYDP) in two media [sodium dodecyl sulfate (SDS) and human serum albumin (HSA)]. Energy transfer parameters, photophysical and thermodynamic parameters of probe were determined. We investigated cytotoxicity of PYDP against colorectal adenocarcinoma cell lines (HT-29), breast cancer cell lines (MCF-7) and 3T3-L1 adipocytes (3T3 L1) cells. The cell staining property of PYDP was monitored using a confocal microscope. The results showed that PYDP binds to HSA, bindings are due to electrostatic/ionic interactions, and the binding process is spontaneous. PYDP was found to exhibit negligible cytotoxicity at high concentrations, and confocal microscope images showed that PYDP stained the cytoplasm of MCF-7 cells. © 2023 The Authors. ChemistryOpen published by Wiley-VCH GmbH.
dc.description.sponsorshipWe are grateful to Dr. Ahmet Yaşar for his assistance in the synthesis and characterization of the molecule (PYDP) in the study and to Dr. Ahmet Altay for his supporting in the cytotoxicity study. This work supported by research foundation of Erzincan Binali Yıldırım University (grant number: FYL‐2019‐597).
dc.identifier.doi10.1002/open.202300092
dc.identifier.issn2191-1363
dc.identifier.issue9
dc.identifier.pmcidPMC10477408
dc.identifier.pmid37667461
dc.identifier.scopus2-s2.0-85169678390
dc.identifier.startpagee202300092
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/ebyu/12234
dc.identifier.volume12
dc.language.isoen
dc.publisherJohn Wiley and Sons Inc
dc.relation.ispartofChemistryOpen
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiophysics
dc.subjectcell imaging
dc.subjectcell staining
dc.subjectcytotoxicity
dc.subjectFRET
dc.subjectthermodynamic
dc.subject3T3-L1 Cells
dc.subjectAdenocarcinoma
dc.subjectAnimals
dc.subjectEnergy Transfer
dc.subjectFluorescent Dyes
dc.subjectHT29 Cells
dc.subjectHumans
dc.subjectMice
dc.subjectSerum Albumin, Human
dc.subjectCell culture
dc.subjectFluorescence spectroscopy
dc.subjectForster resonance energy transfer
dc.subjectProbes
dc.subjectSodium dodecyl sulfate
dc.subjectSulfur compounds
dc.subjectfluorescent dye
dc.subjecthuman serum albumin
dc.subjectBiological interactions
dc.subjectEnergy cells
dc.subjectEnergy-transfer
dc.subjectFluorescent probes
dc.subjectHuman serum albumins
dc.subjectProperty
dc.subjectPyrazolines
dc.titleExploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining Applications
dc.typeArticle
dspace.entity.typePublication
rdl.citation.scopus1
rdl.departmentFaculty of Arts and Sciences, Department of Chemistry, Erzincan Binali Yıldırım University, Erzincan, Türkiye.
rdl.oa.statusgold
rdl.source.indexSCOPUS
rdl.source.indexPUBMED