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Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)

ArticleAçık Erişim

Özet

In 2019, the World Health Organization declared 3 billion to be at risk of developing Crimean Congo Hemorrhagic Fever (CCHF). The causative agent of this deadly infection is CCHFV. The data related to the biology and immunology of CCHFV are rather scarce. Due to its indispensable roles in the viral life cycle, NP becomes a logical target for detailed viral immunology studies. In this study, humoral immunity to NP was investigated in CCHF survivors, as well as in immunized mice and rabbits. Abundant antibody response against NP was demonstrated both during natural infection in humans and following experimental immunizations in mice and rabbits. Also, cellular immune responses to recombinant NP (rNP) was detected in multispecies. This study represents the most comprehensive investigation on NP as an inducer of both humoral and cellular immunity in multiple hosts and proves that rNP is an excellent candidate warranting further immunological studies specifically on vaccine investigations. © 2021 Karaaslan et al.

Anahtar Kelimeler

AnimalsAntibodies, ViralCytokinesHemorrhagic Fever Virus, Crimean-CongoHemorrhagic Fever, CrimeanHumansImmunityImmunity, HumoralImmunizationMaleMiceMice, Inbred BALB CNucleocapsid ProteinsRabbitsaluminum hydroxidebovine serum albumincytokineformaldehydeimmunoglobulin G antibodyisofluranenucleocapsid proteinpenicillin derivativephosphate buffered salinepyroxylinstreptomycinvirus antibodyanesthesiaanimal cellanimal experimentanimal model
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Tam Kayıt
AlanDeğerDil
dc.contributor.authorKaraaslan, Elif
dc.contributor.authorÇetin, Nesibe Selma
dc.contributor.authorKalkan-Yazıcı, Merve
dc.contributor.authorHasanoğlu, Sevde
dc.contributor.authorKarakeçili, Faruk
dc.contributor.authorÖzdarendeli, Aykut
dc.contributor.authorKalkan, Ahmet
dc.contributor.authorKılıç, Ali Osman
dc.contributor.authorDoymaz, Mehmet Ziya
dc.date.accessioned2026-10-18T20:28:04.922860555Z
dc.date.available2026-10-18T20:28:04.922860555Z
dc.date.issued2021
dc.description.abstractIn 2019, the World Health Organization declared 3 billion to be at risk of developing Crimean Congo Hemorrhagic Fever (CCHF). The causative agent of this deadly infection is CCHFV. The data related to the biology and immunology of CCHFV are rather scarce. Due to its indispensable roles in the viral life cycle, NP becomes a logical target for detailed viral immunology studies. In this study, humoral immunity to NP was investigated in CCHF survivors, as well as in immunized mice and rabbits. Abundant antibody response against NP was demonstrated both during natural infection in humans and following experimental immunizations in mice and rabbits. Also, cellular immune responses to recombinant NP (rNP) was detected in multispecies. This study represents the most comprehensive investigation on NP as an inducer of both humoral and cellular immunity in multiple hosts and proves that rNP is an excellent candidate warranting further immunological studies specifically on vaccine investigations. © 2021 Karaaslan et al.
dc.description.sponsorshipThis work was supported in part by the Scientific and Technological Research Council of Turkey (TÜBİTAK) [grant number 213S132 (MZD)], and by the Bezmialem Vakif University Scientific Research Projects (BAP) [grant numbers 3.2014/1 and 3.2014/2 (MZD)]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.identifier.doi10.1371/journal.pntd.0009973
dc.identifier.issn1935-2727
dc.identifier.issue12
dc.identifier.pmcidPMC8635347
dc.identifier.pmid34851958
dc.identifier.scopus2-s2.0-85122269173
dc.identifier.startpagee0009973
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/ebyu/13239
dc.identifier.volume15
dc.identifier.wosWOS:000750645700007
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.ispartofPLoS Neglected Tropical Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnimals
dc.subjectAntibodies, Viral
dc.subjectCytokines
dc.subjectHemorrhagic Fever Virus, Crimean-Congo
dc.subjectHemorrhagic Fever, Crimean
dc.subjectHumans
dc.subjectImmunity
dc.subjectImmunity, Humoral
dc.subjectImmunization
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectNucleocapsid Proteins
dc.subjectRabbits
dc.subjectaluminum hydroxide
dc.subjectbovine serum albumin
dc.subjectcytokine
dc.subjectformaldehyde
dc.subjectimmunoglobulin G antibody
dc.subjectisoflurane
dc.subjectnucleocapsid protein
dc.subjectpenicillin derivative
dc.subjectphosphate buffered saline
dc.subjectpyroxylin
dc.subjectstreptomycin
dc.subjectvirus antibody
dc.subjectanesthesia
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.titleImmune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
dc.typeArticle
dspace.entity.typePublication
rdl.citation.scopus27
rdl.citation.wos25
rdl.departmentDepartment of Infectious Diseases and Clinical Microbiology, Erzincan University School of Medicine, Erzincan, Turkey.
rdl.oa.statusgold
rdl.source.indexSCOPUS
rdl.source.indexWOS
rdl.source.indexPUBMED
rdl.wos.indexScience Citation Index Expanded (SCI-EXPANDED)