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In vitro effects of some drugs on catalase purified from human skin

ArticleAçık Erişim

Özet

Catalase enzyme (H2O2: oxidoreductase; E.C. 1.11.1.6) was purified from human skin homogenate using ammonium sulfate precipitation and DEAE-Sephadex A50 ion exchange chromatography at 4°C and some characteristics of the enzyme were investigated. The human skin enzyme, having a specific activity of 1354.5 EU/mg proteins was purified with a yield of 43.13% and 1110-fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band for the enzyme. Inhibition by piroxicam, ketoprofen, diclofenac sodium, sulfamethoxazole and nidazole occurred with I 50 values of 0.414, 1.29, 1.8, 3.83, and 8.64 mM, respectively. © 2006 Taylor & Francis.

Anahtar Kelimeler

CatalaseDrugHuman skinInhibitionPurificationAnti-Inflammatory Agents, Non-SteroidalDiclofenacDose-Response Relationship, DrugEnzyme InhibitorsHumansInhibitory Concentration 50KetoprofenPiroxicamSkinSulfamethoxazoleammonium sulfatemetronidazolearticleconcentration responsecontrolled studydrug mechanismenzyme analysisenzyme inhibitionenzyme purificationenzyme specificityhumanhuman tissueIC 50in vitro studyinhibition kinetics
RDL Kalıcı Bağlantı (PID)
https://rdlopen.rawdatalibrary.net/r/ebyu/16042Resmi atıflarda kullanılan kalıcı adres
DOI
https://doi.org/10.1080/14756360500483453Yayıncı resmi DOI adresi
Tam Kayıt
AlanDeğerDil
dc.contributor.authorAltikat, Sayit
dc.contributor.authorCoban, Abdulkadir
dc.contributor.authorCiftci, Mehmet
dc.contributor.authorOzdemir, Hasan
dc.date.accessioned2026-10-18T20:30:52.765331272Z
dc.date.available2026-10-18T20:30:52.765331272Z
dc.date.issued2006
dc.description.abstractCatalase enzyme (H2O2: oxidoreductase; E.C. 1.11.1.6) was purified from human skin homogenate using ammonium sulfate precipitation and DEAE-Sephadex A50 ion exchange chromatography at 4°C and some characteristics of the enzyme were investigated. The human skin enzyme, having a specific activity of 1354.5 EU/mg proteins was purified with a yield of 43.13% and 1110-fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band for the enzyme. Inhibition by piroxicam, ketoprofen, diclofenac sodium, sulfamethoxazole and nidazole occurred with I 50 values of 0.414, 1.29, 1.8, 3.83, and 8.64 mM, respectively. © 2006 Taylor & Francis.
dc.identifier.doi10.1080/14756360500483453
dc.identifier.endpage234
dc.identifier.issn1475-6374
dc.identifier.issue2
dc.identifier.pmid16789438
dc.identifier.scopus2-s2.0-33746139318
dc.identifier.startpage231
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/ebyu/16042
dc.identifier.volume21
dc.language.isoen
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCatalase
dc.subjectDrug
dc.subjectHuman skin
dc.subjectInhibition
dc.subjectPurification
dc.subjectAnti-Inflammatory Agents, Non-Steroidal
dc.subjectDiclofenac
dc.subjectDose-Response Relationship, Drug
dc.subjectEnzyme Inhibitors
dc.subjectHumans
dc.subjectInhibitory Concentration 50
dc.subjectKetoprofen
dc.subjectPiroxicam
dc.subjectSkin
dc.subjectSulfamethoxazole
dc.subjectammonium sulfate
dc.subjectmetronidazole
dc.subjectarticle
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdrug mechanism
dc.subjectenzyme analysis
dc.subjectenzyme inhibition
dc.subjectenzyme purification
dc.subjectenzyme specificity
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectIC 50
dc.subjectin vitro study
dc.subjectinhibition kinetics
dc.titleIn vitro effects of some drugs on catalase purified from human skin
dc.typeArticle
dspace.entity.typePublication
rdl.citation.scopus13
rdl.oa.statusopen
rdl.source.indexSCOPUS