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Investigation of the inhibition effects of some antibiotics on human erythrocyte carbonic anhydrase isozymes

ArticleAçık Erişim

Özet

In this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chromatography. Inhibition effects of four different antibiotics on CA isozymes were determined by using the CO2-Hydratase method. I50 values of antibiotics were obtained by drawing % Activity [Actibiotic] graphs. The obtained I50 values of cefazolin sodium, cephradine, sulbactan/cefoperazone and chloramphenicol sodium succinate were 9, 16, 19 and 48 mM on CA-I and 6, 10, 15 and 17 mM on CA-II, respectively. Antibiotics dosages should be taken into consideration because of the inhibition effect on CA which pH regulatory enzyme in body.

Anahtar Kelimeler

AntibioticsCarbonic anhydrase isozymesInhibitionantibiotic agentcarbonate dehydratasecefazolincefoperazonecefradinechloramphenicol derivativesulbactamacidificationaffinity chromatographyantimicrobial activityarticlebasolateral membraneblood samplingbrush borderdrug effectenzyme activityerythrocyte membranehumanhuman cellkidney tubule absorptionmembrane binding
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Tam Kayıt
AlanDeğerDil
dc.contributor.authorCoban, T.A.
dc.contributor.authorNalbantoglu, B.
dc.contributor.authorCil, M.Y.
dc.contributor.authorOzdemir, H.
dc.contributor.authorKufrevioglu, O.I.
dc.date.accessioned2026-10-18T20:30:59.253336088Z
dc.date.available2026-10-18T20:30:59.253336088Z
dc.date.issued1998
dc.description.abstractIn this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chromatography. Inhibition effects of four different antibiotics on CA isozymes were determined by using the CO2-Hydratase method. I50 values of antibiotics were obtained by drawing % Activity [Actibiotic] graphs. The obtained I50 values of cefazolin sodium, cephradine, sulbactan/cefoperazone and chloramphenicol sodium succinate were 9, 16, 19 and 48 mM on CA-I and 6, 10, 15 and 17 mM on CA-II, respectively. Antibiotics dosages should be taken into consideration because of the inhibition effect on CA which pH regulatory enzyme in body.
dc.identifier.endpage410
dc.identifier.issn1300-0144
dc.identifier.issue4
dc.identifier.scopus2-s2.0-0031734648
dc.identifier.startpage407
dc.identifier.urihttps://rdlopen.rawdatalibrary.net/r/ebyu/16183
dc.identifier.volume28
dc.language.isoen
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntibiotics
dc.subjectCarbonic anhydrase isozymes
dc.subjectInhibition
dc.subjectantibiotic agent
dc.subjectcarbonate dehydratase
dc.subjectcefazolin
dc.subjectcefoperazone
dc.subjectcefradine
dc.subjectchloramphenicol derivative
dc.subjectsulbactam
dc.subjectacidification
dc.subjectaffinity chromatography
dc.subjectantimicrobial activity
dc.subjectarticle
dc.subjectbasolateral membrane
dc.subjectblood sampling
dc.subjectbrush border
dc.subjectdrug effect
dc.subjectenzyme activity
dc.subjecterythrocyte membrane
dc.subjecthuman
dc.subjecthuman cell
dc.subjectkidney tubule absorption
dc.subjectmembrane binding
dc.titleInvestigation of the inhibition effects of some antibiotics on human erythrocyte carbonic anhydrase isozymes
dc.typeArticle
dspace.entity.typePublication
rdl.citation.scopus4
rdl.source.indexSCOPUS