Effect of Lercanidipine, Carvacrol and Their Combination on Ischemia and Reperfusion-induced Distant Organ (Heart) Damage in Rats

Background and Objective: Ischemia/reperfusion (I/R) damage has still been a reason for high morbidity and mortality for patients. Systemic damage is also observed in addition to local damage in lower extremity I/R event. It was purposed to research the protective effect of lercanidipine, carvacrol and lercanidipine, carvacrol their combination (LCC) against cardiac damage possible to develop after the I/R procedure administered to the lower extremities in rats. Materials and Methods: All rats were assigned into five groups, as sham operation (SG), only performed ischemia/reperfusion (I/R) to the lower extremity (IRG), lercanidipine+I/R (LIR), carvacrol+I/R (CIR) and lercanidipine, carvacrol combination+I/R (LCIR), randomly. One hour prior to anesthesia, 10 mg kg(-1) of lercanidipine was given to the LIR group. Carvacrol (50 mg kg(-1)) was injected to the CIR group. Lercanidipine (10 mg kg(-1)) was administered and carvacrol (50 mg kg(-1)) to the LCIR group. In IRG, LIR, CIR and LCIR groups, the ischemia was induced-occluding the femoral-artery for 4 hrs, reperfusion was for 24 hrs. Results: It was observed that malondialdehyde (MDA), troponin I (TPI), Creatine Kinase (CK-MB) and Interleukin 6 (IL-6) levels were lower in the heart tissue applied to LCC and total glutathione, superoxide dismutase and catalase levels were higher than the ones in the groups treated with lercanidipine and carvacrol. Conclusion: Current study suggested that lercanidipine and carvacrol were efficient in the treatment of heart injury, but LCC provided a more efficient treatment. These findings indicated that LCC alone was possible to be better than lercanidipine and carvacrol in reducing the severity of I/R-induced heart damage that occurred in any part of the body.