Güven, Zühal Bayrakçeken; Barlak, Neslişah; Türkmenoğlu, Burçin; Karadayı, Mehmet; Yilmaz, Mustafa Abdullah; Dilek, Esra; Inceer, Huseyin; Cakir, Oguz

Introduction: The genus Tanacetum has a long history of use and is well known for its traditional applications in food, medicine, cosmetics, and agriculture. The biological effects of Tanacetum armenum were investigated, and its phytochemical profiles were analysed. Methods: Phytochemical analysis was conducted using liquid chromatography-mass spectrometry/mass spectrometry, and antioxidant, target enzyme inhibition, and antigenotoxic effects were evaluated. The antiproliferative effects and mechanisms of the extracts were investigated in vitro, and the possible mechanisms of the main compounds were analysed in silico. Results: Phytochemical profile analysis revealed significantly higher levels of chlorogenic acid (64.894 mg/g extract), quinic acid (45.377 mg/g extract), and 1,5-dicaffeoylquinic acid (18.150 mg/g extract). The methanol extract (IC50:116.56 ± 2.84 µg/mL) exhibited strong tyrosinase enzyme inhibition than ethylacetate (IC50:184.04 ± 3.02 µg/mL) and aqueous extracts (IC50:340.84 ± 3.46 µg/mL). The aqueous extract (IC50:328.23 ± 4.22 µg/mL) showed the highest inhibition of acetylcholinesterase, while the methanol extract (IC50:504.87 ± 4.78 µg/mL) demonstrated the highest inhibition of butyrylcholinesterase. The ethylacetate extract exhibited the strongest antimicrobial effect against Staphylococcus aureus, with a MIC value of 62.5 µg/mL. The extracts did not show any genotoxic effects. The ethyl acetate extract exhibited significant cytotoxic effects (IC50:25.00–108.16 µg/mL) against cancer cell lines (p < 0.001). To investigate the mechanism by which the extracts inhibit MCF-7 cell viability, the expression levels of the cyclin E1 and caspase-8 were evaluated using western blot analysis. In addition, a colony formation assay was performed, and migration potential was analysed using a scratch wound healing assay. Using in silico approaches, the molecular docking and binding parameter values of cyclin E1 and caspase-8 were calculated. Chlorogenic acid was found to have the best binding value and mode of in silico interaction with the target crystal structures. The docking scores calculated from these binding parameter values were -7.824 kcal/mol for the cyclin E1 (8H6P) target, -7.126 kcal/mol for the caspase-8 (3KJQ) target, and -8.182 kcal/mol for the human oestrogen receptor (3ERT) target. Conclusion: T. armenum, which has demonstrated a strong cytotoxic effect through in vitro and in silico mechanism studies, can be considered a promising potential natural resource for cancer treatment.

Turan, Gülcan Bahçecioğlu; Özer, Zülfünaz; Yanmış Karaca, Safiye; Mollooğlu, Mukadder; Ersöz, Kübra

Introduction: This study was conducted to examine the effects of color therapy on depression, anxiety, stress levels, and quality of life in individuals receiving hemodialysis treatment. Methods: This study was designed as a two-arm, parallel-group randomized controlled trial with baseline and post-intervention assessments. The study sample consisted of a total of 60 patients receiving hemodialysis treatment at a university hospital dialysis unit. The intervention group received color therapy for eight weeks, while the control group received no intervention. Data were collected before and after the color therapy intervention using Personal Information Form, Depression Anxiety Stress Scale-21, and the Quality of Life Index Dialysis Version III. Results: At the end of the 8-week study period, the color therapy group showed greater improvements than the control group in depression, anxiety, and stress scores. The between-group differences favored color therapy for depression [MD = −6.27, 95% CI −7.29 to −5.25, Cohen's d = 3.175], anxiety [MD = −6.50, 95% CI −7.68 to −5.32, Cohen's d = 2.843], and stress [MD = −7.70, 95% CI −8.73 to −6.67, Cohen's d = 3.870]. Quality of life also improved more in the color therapy group than in the control group [MD = 7.60, 95% CI 6.15 to 9.05, Cohen's d = 2.697]. No intervention-related adverse events were reported. Conclusion: Color therapy is an effective, safe, and feasible complementary nursing intervention that reduces depression, anxiety, and stress levels and improves quality of life in hemodialysis patients. These findings indicate that color therapy can be used as part of holistic nursing care in hemodialysis units and integrated into the nursing process. Clinical Trial Registration: NCT06879808

Yeniçeri, Esma; Altay, Ahmet; Koksal, Ekrem; Altın, Sevgi; Taslimi, Parham; Yılmaz, Mustafa Abdullah; Cakir, Oğuz; Tarhan, Abbas; Kandemir, Ali

Introduction: Studies on different species of Onobrychis, a member of the Fabaceae family, have revealed a number of biological activities with potential applications in alternative medicine. The phytochemical content of the plant Onobrychis argyrea subsp. argyrea and some of the important biological activities linked to its metabolism are quite limited. Therefore, this study was the first to determine the content of secondary metabolites in this species and to elucidate important biological activities related to metabolism. Methods: LC-MS/MS was used for the quantitative analysis of bioactive compounds in the aerial part of O. argyrea extracts. The antioxidant properties of the extracts were assessed based on their ability to scavenge DPPH radicals and reduce iron ions. The anti-diabetic effect of the extracts was tested by the inhibition of α-amylase and α-glycosidase enzymes, and the anti-Alzheimer's ability was tested by the inhibition of the enzymes AChE and BChE. The cytotoxic effects of the extracts were tested on four different cancer lines, namely A-549, HT-29, MCF-7 and MDA-MB 453 using the XTT assay. The mechanism of the anti-cancer effect was determined by means of flow cytometry analysis on HT-29 cells. Results: Twenty-nine phytochemicals were identified via the LC-MS/MS analysis in the plant extracts. The most abundant phytochemicals in the extracts were quinic acid, isoquercitrin, epicatechin, and routine, respectively. Antioxidant analysis showed that among all extracts, methanol extract (ME) was most effective in scavenging DPPH (IC50: 23.93 ± 0.96 µg/mL) and in reducing iron ions (53.24 ± 2.14 mgTEAC/g). Enzyme activity studies demonstrated that ME is a potent inhibitor of α-glycosidase (IC50: 15.06±0.64 µg/mL), AChE (IC50: 24.75±0.76 µg/mL), and BChE (IC50: 3.36±0.14 µg/mL). XTT assay results disclosed the strongest anti-proliferative activity of ME against the human colorectal adenocarcinoma HT-29 cell line with an IC50 value of 33.35±0.92 µg/mL. Flow cytometry analysis revealed that ME caused mitochondrial membrane damage in HT-29 cells (**p<0.01) and subsequently induced apoptosis (**p<0.01) by activating the caspase pathway (**p<0.001). Conclusion: O. argyrea, rich in bioactive compounds, showed remarkable antioxidant, antidiabetic, Alzheimer's, and anticancer properties in vitro, demonstrating that this plant contains secondary metabolites that may be potential drug candidates for the treatment of metabolic diseases.