Akşit, Hüseyin

A phytochemical study on the Potamogeton pusillus L. led to the isolation and identification of a novel biflavonoid, pusillin A (1) with known seven compounds, including rosmarinic acid (2), luteolin-7-sulfate (3), eriodictyol-7-sulfate (4), chrysoeriol-7-sulfate (5), eriodictyol (5), luteolin (7) and chrysoeriol (8). Their structures were elucidated based on spectrometric analysis, including LC-Q-TOF/MS and NMR. All compounds were screened for in vitro antidiabetic activity using the spectrophotometric method. Sulfated flavones (1, 3, 4, and 5) exhibited prominent α-glucosidase inhibitory activity, and compounds 2, 6, 7, and 8 showed moderate inhibitory activities. All isolates were reported for the first time from the plant material.

Köksal, Ekrem; Ulutaş, Yakup; Şimşek, Samed; Bayraktar, Taha; Altay, Ahmet; Çatır, Mustafa; Demirtaş, İbrahim; Tüfekçi, Ali Rıza; Kandemir, Ali; Alp, Cemalettin

Secondary metabolites of Tanacetum alyssifolium (Bornm.) Grierson (Asteraceae) were investigated for the first time. Thirteen compounds including axillarin (1), Luteolin-7-O-β-glucoside (8) and rutin (13) as flavonoids, fraxetin (7), isofraxidin (9), isofraxidin-7-O-glucoside (11) and fraxidin (12) as coumarins, tatridin A (2), altissin (3), tamirin (4) and tanachin (5) as sesquiterpene lactones and 2,4-dihydroxy-6-methoxy acetophenone (6) and picein (10) as acetophenone derivatives were isolated from the methanol extract of the species. The structures of all isolated compounds were elucidated by 1D and 2D-NMR techniques and by comparing with the literature data. In addition, in vitro cytotoxic activity of all isolated compounds was evaluated against breast cancer MCF-7 cell line by XTT assay. Sesquiterpene lactones; tatridin A (2), tamirin (4) and tanachin (5) were found to be the most cytotoxic molecules against MCF-7 cell line.