Erhan, Ertugrul; Mammadov, Renad; Altuner, Durdu; Cimen, Ferda; Kurt, Nezahat; Hacimuftuoglu, Fazil; Turan, Fatih; Terzi, Suat; Celiker, Metin; Suleyman, Halis

No previous studies have examined the effect of Hippophae rhamnoides fruit extract (HRE), which contains both water and fat soluble vitamins, on cisplatin-induced ototoxicity. Therefore, the aim of the study was to investigate the effects of FIRE on cisplatin-induced ototoxicity in Guinea pigs and to evaluate the biochemical, gene expression and histopathological changes associated with cisplatin use. Experimental animals were divided into three groups: healthy (HG), HRE+cisplatin (HRC) and cisplatin control (CCG) groups. Biochemical, gene expression and histopathological examinations were carried on the removed inner ear tissues. In the HRC group, the oxidant parameter was lower and the anti-oxidant parameter was higher than in the CCG. These results are supported by gene expression levels and histopathological results. The use of FIRE against cisplatin-induced oxidative ototoxicity may be easier, more cost effective and more beneficial than the use of vitamins alone or in combination with each other.

Delibas, Ilhan Bahri; Karaca, Ibrahim; Yapca, Omer Erkan; Ingec, Metin; Kumbasar, Serkan; Cimen, Ferda Keskin; Coskun, Resit; Celik, Aziz Inan; Cankaya, Murat; Altuner, Durdu

An ischaemia/reperfusion (I/R) injury in an organ can also cause damage to other organs. In this study, the effects of nizatidine were investigated on ovarian I/R injury and subsequent heart damage in rats. Rats were divided into groups; ovarian I/R (IRG), nizatidine 25 mg/kg+ ovarian I/R (NIR), and control (CG) groups. The NIR and IRG groups were given 2 hours of ischaemia followed by 2 hours of reperfusion by applying, and subsequently releasing, a vascular clip to the right ovarian artery. While oxidant levels in the IRG group were higher than NIR and CG groups, antioxidant levels were lower. Necrosis, congested blood vessels, haemorrhage, leukocyte infiltration and oedema were observed in the ovarian and heart tissues of the IRG group. These findings were reduced in the NIR group. Nizatidine suppressed oxidative stress in ovarian and heart tissues and may be useful in prevention of I/R–induced ovarian and cardiac injury.

Özyurek, Selçuk; Turkyilmaz, Dogan; Yaprak, Mustafa; Esenbuga, Nurinisa

In this study, it was aimed that determination of udder measurements of Morkaraman, Tuj and Awassi ewes and the correlation between the results. Udder depth and width of the Awassi was higher than other breeds and respectively 13.09±0.32 cm, 14.07±0.43 cm. In Morkaraman, teats length (2.65±0.06 cm) and diameter of the teats (1.83±0.06 cm) were higher from other breeds. The important statistical difference was found between breeds for udder depth (P<0.05), very important statistical difference was found between breeds for udder height and teats diameter (P<0.01). In this study, teats length and teats diameter were above the limits specified for machine milking for Morkaraman, Tuj and Awassi.

Kocyigit, R.; Aydin, R.; Yanar, M.; Guler, O.; Diler, A.; Tuzemen, N.; Avci, M.; Ozyurek, S.; Hirik, E.; Kabakci, D.

The objectives of this study were to compare the effect of different doses of direct-fed microbials (DFM) plus exogenous fibrolytic enzymes (EFE) additives on the growth performance, feed efficiency ratio and fecal consistency index of Brown Swiss and Holstein Friesian calves. Twenty six calves were assigned to three groups (control, 10 g and 20 g per head/day of DFM plus EFE) according to breeds. Calves in 10 g per head/day of DFM plus EFE group in pre- and post-weaning periods had 20.0% and 6.3% higher total weight gains than calves in the control group respectively. However, the effects of breeds and dosages of the supplement on the weights, weight gains and feed efficiency ratios were not significant. Average fecal consistency score of the calves fed a diet supplemented with 10 g head/day of DFM plus EFE had the lowest score (P<0.01) (i.e., less scouring) compared to other treatment groups in pre-weaning period as well as between birth and 6 months of age. The study revealed that the feeding of DFM plus EFE to dairy calves until 6 months of age did not result in statistically significant improvement on the growth traits and feed efficiency ratio. On the other hand, the dose of 10 g head/day of the DFM plus EFE additives might be beneficial for reducing incidence of diarrhea in dairy calves.