Bursal, Ercan; Köksal, Ekrem; Gülçin, Ilhami; Bilsel, Gökhan; Gören, Ahmet C.

In this study, the antioxidant and antiradical properties of cherry stem (Cerasus avium L.) were examined. The ferric thiocyanate method, ferric ion (Fe3+) and cupric ion (Cu2+) reducing assays and ferrous ions (Fe3+) chelating assay were used in order to measure the antioxidant activity of the plant. Also, its antiradical activity was measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. Additionally, total phenolic and flavonoid contents of the plant were determined. It was indicated that, both the water extract of cherry stem (WECS) and ethanol extract of cherry stem (EECS) have both antioxidant and antiradical properties, and there is a correlation between these properties and the phenolic and flavonoid contents. Quantities of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC-MS/MS). This study will bring an innovation for further studies conducted on the antioxidant properties of WECS and EECS.

Bursal, Ercan; Köksal, Ekrem

The purpose of this study was to determine the reducing power, metal chelating, and radical scavenging capabilities of water and ethanol extracts of sumac (Rhus coriaria L.), comparatively. The water and ethanol extracts of sumac were evaluated for their radical scavenging activities by means of the DPPH and DMPD assays. Water extract of sumac (R. coriaria L.) scavenged radicals effectively with EC50 values of 36.4μg/ml for DPPH free radical and 44.7μg/ml for DMPD cation radical. Similarly, the total reducing power of water extract was found higher than ethanol extract in both potassium ferricyanide reduction (FRAP) and cupric ions reduction capacity methods (CUPRAC). 2,2'-Bipyridine was used to determine the metal chelating activity and the result of water extract was found higher than ethanol extract. Total phenolic and total flavonoid contents of both extracts were studied as well. The values of water extract were found to be higher than that of ethanol extract. The present study found that water extracts of sumac (R. coriaria L.) have effective antioxidant and radical scavenging activities as compared to ethanol extracts.