Gastroprotective effect of Oxygen-Ozone therapy in the model of indomethacin induced acute gastric ulcer in rats

We evaluated the effects of a panel of pharmaceutical interventions in an experimental model of gastric ulcer. The aim of this study is to investigate the protective effect of ozone therapy in an experimental rat model of acute gastric ulcer induced by indomethacin. Twenty-eight Wistar-albino male rats were used in this study. The rats were divided into four groups of seven animals in group as follows: Group 1 (control), Group 2 (sham), Group 3 (ozone), and Group 4 (lansoprazole). After 24 hours of fasting, 5 ml ozone-oxygen mixture was administered to Group 3, 30 mg/kg lansoprazole-5 ml distilled water mixture was administered to Group 4, and 5 ml distilled water was administered to Group 2 by gavage. Five minutes later, 25 mg/kg of indomethacin was administered to stomachs of all rats by gavage except for the control group. After six hours, all animals were anesthetized and stomach tissue was extracted. Ulcers on the gastric surface were measured at the time of collection. Myeloperoxidase (MPO), malondialdehyde (MDA), catalase (CAT), total glutathione (tGSH), superoxide dismutase (SOD), and 8-hydroxy-deoxyguanosine (8-OHdG) were measured in the stomach tissue. Administration of ozone and lansoprazole led to decreased macroscopic gastric ulcer size induced by indomethacin. Ulcer area was elevated in the sham group (71.47 ± 17.08 mm2) relative to the treatment groups. Ulcer area was 20.79 ± 12.88 mm2 in ozone group, significantly lower than sham group but significantly greater than the lansoprazole group. MPO, MDA, CAT, and 8-OHdG levels after ozone administration were significantly reduced and tGSH and SOD levels were higher relative to the lansoprazole group (P<0.01). Ozone therapy has a protective effect on acute gastric ulcer induced by non-steroidal anti-inflammatory drugs through an antioxidative mechanism. © 2016, E-Century Publishing Corporation. All rights reserved.