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- ArticleAçık Erişim2024Zinc Oxide Nanoparticles: An Influential Element in Alleviating Salt Stress in Quinoa (Chenopodium quinoa L. Cv Atlas)
Türkoğlu, Aras; Haliloğlu, Kamil; Ekinci, Melek; Turan, Metin; Yildirim, Ertan; Öztürk, Halil Ibrahim; Stansluos, Atom Atanasio Ladu; Nadaroğlu, Hayrunnisa; Piekutowska, Magdalena; Niedbała, Gniewko
Climate change has intensified abiotic stresses, notably salinity, detrimentally affecting crop yield. To counter these effects, nanomaterials have emerged as a promising tool to mitigate the adverse impacts on plant growth and development. Specifically, zinc oxide nanoparticles (ZnO-NPs) have demonstrated efficacy in facilitating a gradual release of zinc, thus enhancing its bioavailability to plants. With the goal of ensuring sustainable plant production, our aim was to examine how green-synthesized ZnO-NPs influence the seedling growth of quinoa (Chenopodium quinoa L. Cv Atlas) under conditions of salinity stress. To induce salt stress, solutions with three different NaCl concentrations (0, 100, and 200 mM) were prepared. Additionally, Zn and ZnO-NPs were administered at four different concentrations (0, 50, 100, and 200 ppm). In this study, plant height (cm), plant weight (g), plant diameter (mm), chlorophyll content (SPAD), K/Na value, Ca/Na value, antioxidant enzyme activities (SOD: EU g−1 leaf; CAT: EU g−1 leaf; POD: EU g−1 leaf), H2O2 (mmol kg−1), MDA (nmol g−1 DW), proline (µg g−1 FW), and sucrose (g L−1), content parameters were measured. XRD analysis confirmed the crystalline structure of ZnO nanoparticles with identified planes. Salinity stress significantly reduced plant metrics and altered ion ratios, while increasing oxidative stress indicators and osmolytes. Conversely, Zn and ZnO-NPs mitigated these effects, reducing oxidative damage and enhancing enzyme activities. This supports Zn’s role in limiting salinity uptake and improving physiological responses in quinoa seedlings, suggesting a promising strategy for enhancing crop resilience. Overall, this study underscores nanomaterials’ potential in sustainable agriculture and stress management.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022iPBS-Retrotransposon Markers in the Analysis of Genetic Diversity among Common Bean (Phaseolus vulgaris L.) Germplasm from Türkey
Haliloğlu, Kamil; Türkoğlu, Aras; Öztürk, Halil Ibrahim; Özkan, Güller; Elkoca, Erdal; Poczai, Peter
Beans are legumes that play extremely important roles in human nutrition, serving as good sources of protein, vitamins, minerals, and antioxidants. In this study, we tried to elucidate the genetic diversity and population structure of 40 Turkish bean (Phaseolus vulgaris L.) local varieties and 5 commercial cultivars collected from 8 different locations in Erzurum-Ispir by using inter-primary binding site (iPBS) retrotransposon markers. For molecular characterization, the 26 most polymorphic iPBS primers were used; 52 bands per primer and 1350 bands in total were recorded. The mean polymorphism information content was 0.331. Various diversity indices, such as the mean effective allele number (0.706), mean Shannon’s information index (0.546), and gene diversity (0.361) revealed the presence of sufficient genetic diversity in the germplasm examined. Molecular analysis of variance (AMOVA) revealed that 67% of variation in bean germplasm was due to differ-ences within populations. In addition, population structure analysis exposed all local and commercial bean varieties from five sub-populations. Expected heterozygosity values ranged between 0.1567 (the fourth sub-population) and 0.3210 (first sub-population), with an average value of 0.2103. In contrary, population differentiation measurement (Fst) was identified as 0.0062 for the first sub-population, 0.6372 for the fourth subpopulations. This is the first study to investigate the genetic diversity and population structure of bean germplasm in Erzurum-Ispir region using the iPBS-retrotransposon marker system. Overall, the current results showed that iPBS markers could be used consistently to elucidate the genetic diversity of local and commercial bean varieties and potentially be included in future studies examining diversity in a larger collection of local and commercial bean varieties from different regions.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)
Türkoğlu, Aras; Haliloğlu, Kamil; Balpinar, Özge; Öztürk, Halil Ibrahim; Özkan, Güller; Poczai, Peter
Mammalian sex hormones are steroid-structured compounds that support the growth and development of plants at low concentrations. Since they affect the physiological processes in plants, it has been thought that mammalian sex hormones may cause modifications to plant genomes and epigenetics. This study aims to determine whether different mammalian sex hormones (17 β-estradiol, estrogen, progesterone, and testosterone) in several concentrations (0, 10−4, 10−6, and 10−8 mM) affect genetic or epigenetic levels in bean plants, using in vitro tissue cultures from plumule explants. We investigated levels of DNA damage, changes in DNA methylation and DNA stability in common bean exposed to mammalian sex hormones (MSH) using inter-primer binding site (iPBS) and Coupled Restriction Enzyme Digestion-iPBS (CRED-iPBS) assays, respectively. The highest rate of polymorphism in iPBS profiles was observed when 10−4 mM of estrogen (52.2%) hormone was administered. This finding indicates that genetic stability is reduced. In the CRED-iPBS profile, which reveals the methylation level associated with the DNA cytosine nucleotide, 10−4 mM of estrogen hormone exhibited the highest hypermethylation value. Polymorphism was observed in all hormone administrations compared to the control (without hormone), and it was determined that genomic stability was decreased at high concentrations. Taken together, the results indicate that 17 β-estradiol, estrogen, progesterone, and testosterone in bean plants affect genomic instability and cause epigenetic modifications, which is an important control mechanism in gene expression.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling via Morphological and Molecular Methods
Bulut, Huseyin; Öztürk, Halil Ibrahim; Dursun, Atilla
Aim of this study was to determine t he effect of tebuconazole applications on cucumber seedling via morphological. and molecular methods. In order to determine the most appropriate dose of different concentrations (25. 50, 75, 100. 125 and 150 ppm) of tebuconazole affecting the seedling quality and preventing possible genotoxic effects, retrotransposon motility at all doses was determined. Polymorphism rates and changes in GTS values were also determined. At the applied doses, polymorphism values were found as 0%. 5.55%. 16.66%, 38.88%, 55.55% and 61.11%, respectively. The GTS value was decreased from 100% to approximately 38.89% based on the applied doses. Statistical evaluations indicated that different dose applications resulted significant changes in the seedling characteristics. The seedling and stem heights were suppressed by 57.5% and 55.9%, respectively compared to the controls. The stem diameter increased 9.95% following the application. Overall. 50, 75 and 150 ppm doses were determined to achieve an increase in the dry matter and leaf chlorophyll content,. In this study, applications of Tebuconazole at different doses controled the seedling height in cucumber and affected specific quality characteristics of the seedlings.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020New Approach in Obtaining the Ideal Watermelon (Citrullus lanatus) Seedling: Tebuconazole
Bulut, Huseyin; Öztürk, Halil Ibrahim
The study was conducted in March-May 2020 in greenhouse in Erzincan Horticultural Research Institute. Sahra F1 variety of watermelon (Citrulus lanatus) was used as plant material in the experiment. In this study, the effects of different doses (0, 25, 50, 75, 100, 125, 150 and 175 ppm) of Tebuconazole substance on the quality and development of watermelon seedlings were investigated. It was determined that different dose applications caused a significant change in seedling characteristics According to the data obtained, a average reduction of 38.45% and 17.81% was achieved in seedling length and stem length, respectively, compared to the control application. Also, the stem diameter increased in 3.03% after the applications. The all doses applications also provided an increase in leaf chlorophyll content (SPAD). In this study, applications of Tebuconazole at different doses were found to control the seedling height in watermelon and positively affected on specific quality characteristics of seedlings.
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