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- ArticleYayıncıda açık erişim2025Inoculating maize (Zea mays L.) seeds with halotolerant rhizobacteria from wild halophytes improves physiological and biochemical responses of seedlings to salt stress
Atici, Ökkeş; Aydın, İhsan; Karakus, Sinem; Tiryaki, Deniz
Salinity stress is a major environmental factor that poses a significant constraint to plant growth, threatening agricultural productivity and sustainability. This study investigated the potential of halotolerant bacteria, isolated from the rhizosphere of wild halotolerant plants in Turkey’s Salt Lake basin, to enhance salt stress tolerance in maize (Zea mays L.). The rhizospheres of 17 different wild halotolerant plants were selected for bacterial isolation, resulting in the identification of 22 halotolerant bacteria using 16S rRNA sequence analysis. Among these, 19 isolates were found to possess positive activity for 1-aminocyclopropane-1-carboxylate (ACC) deaminase and nitrogen fixation. When the maize seeds inoculated with these 19 isolates were grown under normal conditions, four isolates‒TG-4 (Halomonas arcis), TG-8 (Marinococcus tarigensis), TG-12 (Halobacillus dabanensis), and TG-20 (Halomonas eurihalina)–significantly stimulated seedling growth and development. To evaluate the effect of these four isolates on salt tolerance, inoculated seeds were grown under various salt conditions (0.0, 75, 150, and 250 mM NaCl). The responses of plants to salt stress were analyzed by evaluating growth parameters, membrane damage, photosynthetic pigment and proline content, reactive oxygen species and lipid peroxidation levels, and antioxidant enzyme activities. According to the parameters, the results indicated that TG-4, TG-8, and TG-12, in particular, have the potential to function as plant growth-promoting rhizobacteria and effectively enhance salt stress tolerance in the maize seedlings. Overall, this research highlights the potential of halotolerant bacteria to improve salt stress tolerance in maize plants through multifaceted mechanisms, offering valuable insights for sustainable agriculture in saline environments.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2013Alleviation of high salt toxicity-induced oxidative damage by salicylic acid pretreatment in two wheat cultivars
Mutlu, Salih; Atici, Ökkeş
Role of exogenous salicylic acid (SA) in the antioxidative response to salt toxicity of two wheat (Triticum aestivum) cultivars (Gerek-79 and Bezostaya) was investigated. Hydroponic growth environment of 10-day wheat seedlings grown under normal conditions (22/20°C) was adjusted to 0.0, 0.25, 0.50 and 0.75 M of salt (NaCl), and then freshly prepared SA solutions (0.0, 0.01 and 0.1 mM) were once sprayed on leaves of the same seedlings. Activities of superoxide dismutase (SOD), peroxidase (POX) and catalase (CAT) were determined in fresh leaves obtained from 15-day seedlings. Treatment with SA alone increased all the antioxidant activities in Gerek-79. However, the same treatments with SA decreased the activities of CAT and SOD in Bezostaya, while increased that of POX. Salt applications alone increased the activities of POX and SOD, while decreased that of CAT in Bezostaya. The same salt applications increased the POX activity, while decreased the activities of CAT and SOD in Gerek-79. In plants under saline conditions, except 0.75 M NaCl, treatments with SA increased the activities of CAT, POX and SOD in both the varieties compared with plants applied salt alone. In plants applied with 0.75 M NaCl, however, treatments with SA decreased CAT activity. Results showed that salt toxicity caused a derangement in the regulation of antioxidant enzyme activities by decreasing CAT activity especially in both the varieties, but treatments with SA could turn back the derangement in the antioxidative enzymes caused by salt toxicity. SA can involve in increasing salt tolerance by regulating the activities of antioxidant enzymes in wheat cultivars exposed to salt toxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2013Genotoxic effects of catmint (Nepeta meyeri Benth.) essential oils on some weed and crop plants
Kekeç, Güzin; Mutlu, Salih; Alpsoy, Lokman; Sakçali, M. Serdal; Atici, Ökkeş
This study investigates the genotoxicity of the essential oils extracted from the aerial parts of catmint (Nepeta meyeri Benth.) against two weeds (Bromus danthoniae and Lactuca serriola) and two crop plants (Brassica napus and Zea mays). The essential oils of N. meyeri analyzed by gas chromatography–mass spectrometry contained 14 compounds, with 4aα, 7α, 7aβ-nepetalactone (83.4%), 4aα, 7α, and 7aα-nepetalactone (8.83%) as the major components. The oils were diluted (25, 50, 100, and 150 ppm) and the solutions were applied to seeds or leaves of these plants. The study compared the germination percentage and random amplified polymorphic DNA (RAPD) results with the control group. The results showed that the oils had a strong inhibitory activity and caused a change in RAPD profiles in terms of variation in band intensity, loss of bands, and appearance of new bands compared with the control group. The results suggested that RAPD analysis could be applied as a suitable biomarker assay for the detection of genotoxic effects of plant allelochemicals. This study indicates the genotoxical potential of N. meyeri essential oils on weed and crop plants.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2013Cement dust pollution induces toxicity or deficiency of some essential elements in wild plants growing around a cement factory
Mutlu, Salih; Atici, Ökkeş; Gülen, Yasir
In the present study, it was aimed to determine the effects of cement dust pollution on contents of some significant essential elements (P, S, K, Ca, Fe and Cl) in wild plants (Medigago varia, Anchusa leptophylla, Euphorbia orientalis, Lactuca serriola, Artemisia spicigera, Crambe orientalis, Convolvulus sepium and Senecio vernalis) using wavelength-dispersive spectrometer X-ray fluorescence technique. Plant samples were collected from different locations around a cement factory which is located at Askale about 50 km from Erzurum (Turkey). The element contents in the plant specimens that existed in both 0–100 m (dense dusted) and 2000 m (undusted) areas were compared. P, S, K and Cl contents were found to be high in the plants growing in areas 0–100 m from the cement factory, compared to same plants at 2000 m far from the factory. However, Ca and Fe contents were determined to be low in plants growing in 0–100 m area from the factory. Results of the study can contribute to understand how mineral deficiency and toxicity lead to detrimental effects on plant growth and development in the fields contaminated by cement dust.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2013Time-dependent effect of salicylic acid on alleviating cold damage in two barley cultivars differing in cold tolerance
Mutlu, Salih; Karadaǧoǧlu, Ömer; Atici, Ökkeş; Taşǧin, Esen; Nalbantoǧlu, Barbaros
The time-dependent effect of salicylic acid (SA) on alleviating cold damage in the Tokak (tolerant) and Akhisar (sensitive) varieties of barley (Hordeum vulgare) was investigated. Seedlings subjected to 0.0, 0.1, and 1 mM SA for 7 days were transferred to cold (7/5 °C) on days 7, 14, 21, and 28 for 3 days. It was found that 0.1 mM SA had a greater alleviating effect, decreasing the freezing injury in both varieties for at least 10 days after the application. Moreover, 0.1 mM SA had a greater effect on alleviation in Tokak by increasing the ice nucleation activity of apoplastic proteins extracted from the leaves. SA was effective for at least 24 days in decreasing the effect of freezing temperature by regulating apoplastic proteins in the leaves after the application. Furthermore, 0.1 and 1 mM SA had an alleviating effect by decreasing the lipid peroxidation level in both varieties for at least 24 days after the application. It can be seen that SA treatment can play a positive role in alleviating cold damage in barley. Therefore, it is suggested that results from SA treatments may contribute to research related to diminishing cold damage in agricultural applications.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2011Essential oils of catmint (Nepeta meyeri Benth.) induce oxidative stress in early seedlings of various weed species
Mutlu, Salih; Atici, Ökkeş; Esim, Nevzat; Mete, Ebru
The essential oils from the aerial parts of catmint (Nepeta meyeri Benth.) were analyzed by hydrodistillation with GC-MS. Fourteen compounds were identified in the yellowish essential oil of the plant, representing more than 99.07% of the oil, of which the major components were found to be 4aα,7α,7aβ-nepetalactone (83.4%) and 4aα,7α,7aα-nepetalactone (8.83%). The oils were characterized by relatively high content of oxygenated monoterpenes, and were tested on the germination and antioxidative systems in early seedlings of seven weed species (Amaranthus retroflexus L., Bromus danthoniae Trin., Bromus intermedius Guss., Chenopodium album L., Cynodon dactylon L., Lactuca serriola L., and Portulaca oleracea L.) and autotoxicity. The essential oil of N. meyeri inhibited seed germination by more than 50% in three weed species (B. danthoniae, B. intermedius, and L. serriola) when applied at a concentration of 0.01%. When the same oils were applied at 0.02% concentration, the inhibition of germination was more than 70% in two weeds (C. album and C. dactylon) and was 100% in four weeds (A. retroflexus, B. danthoniae, B. intermedius, and L. serriola). The essential oils increased CAT activity in all the weed species and decreased SOD activity, except in A. retroflexus. POX activity did not exhibit a revealing situation in the weed species tested. The essential oils increased the level of lipid peroxidation and hydrogen peroxide (H2O2) concentration in all the weeds studied. Our results show that the essential oils of N. meyeri have an important phytotoxic effect on seed germination and, consequently, seedling growth by exhausting antioxidative system of the weeds. The phytotoxic activity of the essential oils may be attributed to their relatively high content of oxygenated monoterpenes, especially 4aα,7α,7aβ-nepetalactone. It can be suggest that the essential oils of N. meyeri have the potential to be used as a bioherbicide.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2009Effect of cement dust on diversity and antioxidant enzyme activities of plants growing around a cement factory
Mutlu, Salih; Atici, Ökkeş; Kaya, Yusuf
The present study investigated the effect of cement dust on diversity and antioxidant enzyme activities of plants growing around a cement factory. Thirty-six plant species were identified at a distance of 2,000 m from the outer zone of a cement factory (Askale-Erzurum, Turkey). Only 12 of these species were also found within 0-100 m (densely dusted area) of the cement factory. In order to evaluate the antioxidative responses of these 12 species within this dense-dusted area, the activities of peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT) were determined within the plant leaves. In Artemisia spicigera, Crambe orientalis, and Convolvulus sepium plants, the activities of POX and SOD were low while that of CAT was high. In contrast, POX and SOD activity was high in Medicago varia, Isatis candolleana, and Astragalus christianus plants, while that of CAT was low. In Lactuca seriola and Senecio vernalis, however, the activity levels of the three enzymes were interestingly low. In four remaining species (Anchusa leptophylla, Euphorbia orientalis, Taraxacum androssovii and Tragopogon albinervis), the enzyme activities fluctuated between the plants collected in 0-100 m sample area, and those from the sample site 2,000 m from the factory. These findings suggest that Artemisia spicigera, Crambe orientalis, and Convolvulus sepium have a more effective defense system than the other species studied. The findings of this study will contribute to the selection of plant species for cultivation in cement-dust contaminated fields.
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