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- ArticleYalnız künye2022Effect of Usnea longisima Ethyl Acetate Extract on Staphylococcus aureus-Induced Experimental Rhinosinusitis in Rats
Dilber, Muhammet; Erhan, Ertugrul; Bulut, Seval; Kuzucu, Mehmet; Akyuz, Sumeyye; Suleyman, Halis
SUMMARY. The role of oxidant and proinflammatory cytokines in the pathogenesis of rhinosinusitis induced by Staphylococcus aureus has been demonstrated in studies. Usnea longissima ethyl acetate extract (ULE) was obtained from this lichen species. ULE is known to have antibacterial, antioxidant and anti-inflammatory effects. The objective of the study is to investigate the effect of ULE on S. aureus-induced experimental rhinosinusity in rats and compare it with cefazolin. Anesthesia was created through virtue of intraperitoneal injection of 25 mg/kg thiopental sodium to male albino Wistar rats. Then the rats were divided into groups as healthy (HG), ones inoculated with S. aureus (RSG) to their nostril, ones inoculated with S. aureus to their nostril + ULE (REA) applied and ones with S. aureus inoculated to their nostril + cefazolin (RCG) applied. Our biochemical test results revealed the fact that ULE was able to inhibit inflammatory mediators such as nuclear factor kappa B (NF-κB), tumor necrosis factor alpha (TNF-α), an interleukin 1 beta (IL-1β) and cyclooxygenase-2 (COX-2) increase with S. aureus in sinonasal tissue better than cefazolin. Moreover, while ULE significantly suppressed the increase in the amount of malondialdehyde (MDA), the oxidant parameter in the sinonasal tissue, and the decrease in the antioxidant total glutathione (tGSH) and cyclooxygenase-1 (COX-1), cefazolin could not. ULE and cefazolin were observed to be effective on standard bacteria. From our experimental results, it is understood that ULE prevents oxidative and inflammatory damage induced by S. aureus in the sinonasal tissue better than cefazolin.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2021Effect of Taxifolin on Ischemia/Reperfusion-Induced Oxidative Injury of Sciatic Nerve in Rats
Yuceli, Sahin; Suleyman, Bahadir; Yazici, Gulce Naz; Mammadov, Renad; Cankaya, Murat; Kunak, Celaleddin Semih; Bulut, Seval; Suleyman, Halis; Altuner, Durdu
Background: Ischemia is a condition in which blood flow to tissues is decreased or entirely stopped for various reasons. The reperfusion process exacerbates damage caused by ischemia in the organs and tissues. Reactive oxygen species (ROS) are mainly responsible for ischemia-reperfusion (IR) damage. ROS increase results in lipid peroxidation (LPO) and oxidative stress. In the literature, taxifolin reportedly suppresses ROS production. This study aimed to determine the effect of taxifolin, which is a flavonoid, on IR injury of the sciatic nerve in rats. Methods: This study divided 30 albino Wistar rats into 3 groups: IR without medication (IR) group, taxifolin applied IR (TAX+IR) group, and only dissection made to the sciatic nerve sham group (SHAM). Sciatic nerve injury was induced by applying 2 hours of ischemia and 3 hours of reperfusion to the abdominal aorta and iliolumbar arteries. Biochemical and histopathologic investigations then were performed on sciatic nerve tissues. Malondialdehyde, total glutathione, glutathione reductase, and glutathione peroxidase were analyzed as oxidative stress markers, and tumor necrosis factor–α and interleukin-1β levels were evaluated as inflammatory stress markers in biochemical tests. Results: The IR group has statistically significantly high oxidant and cytokine levels and low antioxidant levels compared with the TAX+IR group. Taxifolin treatment was also shown to cause significant histopathologic improvement. Conclusions: We suggest that taxifolin may be effective in preventing IR injury of the sciatic nerve.
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