Sürdürülebilir Kalkınma Amaçları
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- ArticleAçık Erişim2018Chemical applications, scarification and stratification effects on seed germination of rare endemic Verbascum calycosum hausskn. ex Murb. (Scrophulariaceae)
Hilooğlu, Muhip; Sözen, Emel; Yücel, Ersin; Kandemir, Ali
Verbascum calycosum is an endemic plant species having considerable narrow distribution in Erzincan (Turkey) region. This species is known from only a single population and its habitats are highly threatened due to intensive human activities and soil erosion. In this study, the germination behavior of V. calycosum under different concentrations of NaCl, HCl, KNO3, GA3 (100 and 200 μM), hot-cold stratification and mechanical scarification were investigated. Seeds were exposed to a photoperiod of 8 h light/16 h dark with a 23/18 °C thermoperiod. Germination rates increased with GA3-100 μM (39%), GA3-200 μM (54.5%), mechanical scarification (34.5%) and cold stratification treatments (+4 °C, 23.25%; -20 °C, 18.25%) on the other hand, KNO3, NaCl, HCl and stratification with hot water treatments have decreased germination rates significantly when compared to the control (12.25%). Increased germination after GA3 application and mechanical scarification indicated that seeds of V. calycosum exhibited both non-deep and intermediate physiological dormancy as well as physical dormancy due to its hard seed coat. The highest speed of germination index was obtained at cold stratification of +4 °C and -20 °C (10.3). This study represents first report about seed dormancy and germination characteristics of V. calycosum. Overall, these results will provide valuable data for ex situ conservation of this rare endemic plant.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2017Soil-Plant Relationships for Endemic Teucrium leucophyllum Montbret & Aucher ex Bentham (Lamiaceae)
Hilooglu, Muhip; Yücel, Ersin; Kandemir, Ali; Sözen, Emel
Teucrium leucophyllum Montbret & Aucher ex Bentham (Lamiaceae) is an endemic plant species showing narrow distribution in Erzincan region. According to the IUCN criteria, this species wasreported as EX (Extinct) for many years. However, this plant was rediscovered in its original localities during the field studies in the region. In this study, physical and chemical characteristics of soils where T. leucophyllum distributed were determined. The soil type of this species distributed areas were loamy, between 5-7.5YR in color, very dry, slightly salty, slightly alkaline and high in organic matter. In addition, correlation between micro and macro element contents of plant and soil samples were studied. Obtained results will provide valuable data for ex-situ conservation studies for rare and narrow endemic species of T. leucophyllum.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2017Genetic diversity of local endemic Teucrium leucophyllum montbret & aucher ex Bentham. (Lamiaceae) in Turkey
Sözen, Emel; Hilooğlu, Muhip; Kandemir, Ali
Background and Objective: Teucrium leucophyllum is endemic plant species having considerable narrow distribution in Erzincan (Turkey) Region. The aim of this study was to determine genetic diversity levels of T. leucophyllum in order to obtain data for its conservation. Method: Genomic DNA was isolated from 81 plants using CTAB method. Genetic diversity in four natural populations of T. leucophyllum was investigated by ISSR markers. A binary matrix was produced by scoring each amplified fragment as present (1) or absent (0) from each individual. The matrix was used to produce an input file and analyzed using the software programs; POPGENE 1.32 and GenAlex. Results: Twenty primers amplified a total of 434 bright and discernible bands of 220-3000 bp, of which 431 bands (PPB: 99.31%) were polymorphic in 4 populations. The genetic diversity was high at the species level; the observed number of alleles (Na) was 1.993, the effective number of alleles (Ne) was 1.404, the Nei’s genetic diversity (H) was 0.263, and the Shannon’s information index (I) was 0.418. A high level of gene flow (Nm: 2.208) and low-moderate genetic differentiation (Gst: 0.184) were also observed. Analysis of molecular variance showed that 11% of the total genetic diversity resided among populations, while 89% within the populations. Conclusion: Our results indicated that the conservation efforts should aim to preserve the extant populations of this endangered species in its natural habitat. Seeds could be collected from Kayacik and Bagistas populations for ex situ conservation practices.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2016Endemik Onosma discedens tohumlarında İn vitro çimlendirme çalışmaları
Sözen, Emel; Hilooğlu, Muhip; Kandemir, Ali; Yücel, Ersin
Onosma discedens is endemic species having considerable narrow distribution in Erzincan (Turkey) Region. In this study, we investigated the germination behavior of Onosma discedens with different concentrations of NaCI, HCl, KNO3, GA3 (100 and 200 ?M) or hot-cold stratification/mechanical scarification. Seeds under the light and dark treatments were exposed to a photoperiod of 8 h light/16 h dark hours with a 23/18 °C thermoperiod. Germination rates increased with KNO3-100 µM (% 12), GA3-100 µM (% 25), GA3-200 µM (% 35), HCI-100 µM (% 9) treatments when compared to the control. The highest speed of germination index was obtained at concentrations of 100-200 µM KNO3. Overall, our results will provide valuable data for ex situ conservation of endemic Onosma discedens.
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