Sürdürülebilir Kalkınma Amaçları
Yayınlar
- ArticleYalnız künye2014Benefits of the antioxidant and anti-inflammatory activity of etoricoxib in the prevention of ovarian ischemia/reperfusion injury induced experimentally in rats
Yapca, Omer Erkan; Turan, Mehmet Ibrahim; Yilmaz, Ismayil; Salman, Suleyman; Gulapoglu, Mine; Suleyman, Halis
Aim This study is a biochemical investigation of the effect of etoricoxib, a selective cyclooxygenase (COX)-2 inhibitor, on ischemia/reperfusion (I/R) injury experimentally induced in rat ovaries. Methods Experimental animals were divided into four groups: (i) ovarian ischemia/reperfusion (IRG); (ii) 30 mg/kg etoricoxib + ovarian ischemia/reperfusion (EIRG-30); (iii) 60 mg/kg etoricoxib + ovarian ischemia/reperfusion (EIRG-60); and (iv) a sham operation (SG) control group. Results The results showed levels of malondialdehyde in the IRG, EIRG-30, EIRG-60 and SG group ovarian tissue of 20.2 ± 3.4, 11.2 ± 3.2, 5.5 ± 1.9 and 3.8 ± 1.5 μmol/g protein, respectively. Myeloperoxidase activity for these groups was 24.2 ± 6.7, 13 ± 2.4, 4 ± 1.8 and 3.5 ± 1.9 U/g protein, and total glutathione levels were 1.6 ± 0.8, 4.5 ± 1.9, 6.5 ± 1.9 and 7.5 ± 2.4 nmol/g protein, respectively. COX-1 activity in IRG, EIRG-30, EIRG-60 and SG group rat ovarian tissue was 5.0 ± 2.8, 12.2 ± 2.4, 16.7 ± 2.8 and 17.5 ± 4.7 U/mg protein, and COX-2 activity was 18.3 ± 2.7, 3.5 ± 1, 1.8 ± 0.7 and 0.7 ± 0.3 U/mg protein, respectively. Conclusion Etoricoxib prevented oxidative damage induced with I/R in rat ovarian tissue. This property of etoricoxib suggests that it can be clinically beneficial in the prevention of damage that may arise with reperfusion by detorsion for the protection of the ovaries against torsion.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2014Damage induced by paracetamol compared with N-acetylcysteine
Kisaoglu, Abdullah; Ozogul, Bunyami; Turan, Mehmet Ibrahim; Yilmaz, Ismayil; Demiryilmaz, Ismail; Atamanalp, Sabri Selcuk; Bakan, Ebubekir; Suleyman, Halis
Background: This study investigated the effect of thiamine pyrophosphate (TPP) on oxidative liver damage induced in rats with high-dose paracetamol. Methods: Rats for this experiment were divided into the following groups: healthy control, paracetamol control, thiamine+paracetamol, TPP+paracetamol, and N-acetylcysteine+paracetamol. Oxidant and antioxidant parameters and liver function test levels were compared between the groups. Results: The results show that TPP and N-acetylcysteine with paracetamol equally prevented a rise in oxidants such as malondialdehyde and nitric oxide. They also prevented a decrease in enzymatic and nonenzymatic antioxidants such as glutathione, glutathione peroxidase, glutaredoxin, glutathione S-transferase, superoxide dismutase, and catalase in the rat liver. Conclusion: Thiamine pyrophosphate and N-acetylcysteine had a similar positive effect on oxidative damage caused by paracetamol hepatotoxicity. These findings show that TPP may be beneficial in paracetamol hepatotoxicity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2013The investigation of damage in the muscle tissue with the oxidant/antioxidant balance and the extent of postmortem DNA damage in rats
Ozturk, Cengiz; Talip Sener, Mustafa; Sener, Ebru; Yilmaz, Ismayil; Akcay, Fatih; Suleyman, Halis
The relationship of the extent of DNA damage and certain components of the oxidant/antioxidant balance in rat femoral muscle tissue with the post-mortem intervals was investigated and examined by making direct comparison with the histopathological findings. The results have shown that the oxidant/antioxidant balance in the striated muscle tissue to remain constant at the 0 hours post-mortem; to shift mildly in favour of the oxidants at 2 hours post-mortem; to shift moderately in favour of the oxidants at 3 hours post-mortem; and, to shift severely in favour of the oxidants at 4 and 5 hours post-mortem. The amount of DNA damaged products was detected to increase at 4 and 5 post-mortem hours when the oxidant/antioxidant balance became severely shifted. The biochemical findings were found to be in full accordance with the histopathological findings. The above-mentioned findings indicate that DNA becomes subjected to degradation at the hours after post-mortem when the oxidants are strongly predominate.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2013The protective effect of melatonin and agomelatin against cisplatin-induced nephrotoxicity and oxidative stress in the rat kidney
Yilmaz, Ismayil; Demiryilmaz, Ismail; Turan, Mehmet I.; Suleyman, Bahadir; Turan, Isil S.; Altuner, Durdu; Alp, Hamit H.; Suleyman, Halis
Cisplatin is used to treat various types of cancers. Its use is limited, however, due to nephrotoxicity, which may result from free radical damage. Evidence exists that melatonin reduces oxidative stress-induced damage. This study investigated the protective effect of agomelatin, a melatonin receptor agonist, against cisplatin-induced nephrotoxicity and oxidative stress in the rat kidney. Groups of rats were given cisplatin with or without agomelatin or melatonin, or distilled water for 14 days. MDA, tGSH, MPO and 8-OH Gua levels were measured to determine oxidative and DNA damage in renal tissue. Levels of MDA, MPO and 8-OH Gua were lower in the Mel+Cis and Ago+Cis groups than in the Cis group (P < 0.001, P < 0.001, and P < 0.05, respectively). The tGSH level in the Mel+Cis group was higher than that in the Cis group (P < 0.001). Agomelatin and melatonin thus reduced cisplatin-induced oxidative damage and DNA damage in the rat kidney. This suggests that melatonin may be effective in preventing cisplatin nephrotoxicity.
RDL Kalıcı Kaydıİncele