The effect of anakinra on paclitaxel induced auditory nerve damage in rats

In this study, we have examined the protective and proinflammatory effects of anakinra against paclitaxel-associated oxidative auditory nerve damage induced by paclitaxel in rats. Experimental animals were allocated into three groups: Paclitaxel administered controls, paclitaxel + anakinra administered group, and untreated health controls. At the end of this period, animals were sacrificed by high dose thiopental (50 mg/kg) anesthesia and auditory nerves were removed. Then, malondialdehyde, total glutathione, Interleukin-1β and nuclear factor kappa-B levels were measured in the auditory nerve tissue. Our biochemical test results showed that paclitaxel significantly increased the amount of malondialdehyde in auditory nerve tissue compared to anakinra and healthy group (P < 0.0001), whereas total glutathione level decreased. In addition, paclitaxel was found to increase interleukin-1β and nuclear factor kappa-B significantly compared to anakinra and healthy group (P < 0.0001). It was concluded that anakinra is effective in suppressing paclitaxel related oxidative stress and proinflammatory events in auditory nerve tissue. © 2020, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.