The Effect of Ozone Treatment on Thermal Burn Wound Healing; an Experimental Study

Objective: Ozone has been advised as a metabolic excitative in cell, immunomodulatory agent and antioxidant enzyme actuator. The present study was performed to designate the profit of ozone therapy on the burn wound healing in the rats. Methods: 40 adult Wistar type rats divided into 4 groups: a control group (burn not created and topical and systemic agents not used), a burn group (burn created but only saline given), a burn+silver sulfadiazine group (silver sulfadiazine was applied for 21 days), a burn+ozone/oxygen mixture group (ozone/oxygen mixture was applied for 21 days).After anesthetizing, second-degree burn (2 cm2 areas) was done on the dorsal of the animals byaluminum plate in boiling hot water (100 degrees C for 15 seconds) and other groups except control group were treated topically, based on the time scheduled. Tissue samples were harvested on day 7st and 21st after burn injury. Biochemical and histological analyzes were performed in tissue and blood samples. The results were assessed with appropriate statistical tests and given as Means +/- SD. Results: The histopathologic damage level was significantly different in all groups. Fibrosis and inflamation levels decreased in ozone treatment and silver sulphadiazine groups compared to burn+normal saline group on the 21 days according to 7 days. In the ozone treatment group, Glucose 6-phosphate dehydrogenase activities were significantly higher than the silver sulfadiazine treated group. But glutathione reductase enzyme activities were lover in the ozone treated group and hydroxyproline concentration decreased in ozone group compared to burn+normal saline group on 7 and 21 days. Conclusions: Ozone has a detractive effect in the development of inflamation, fibrosis, and granulation via decreasing tissue damage and increasing the antioxidant enzyme activity on burn wound healing.