The Effect of Resveratrol on Ischemia–Reperfusion Induced Oxidative Rat Ovary Injury: A Biochemical, Histopathological, and Genetic Evaluation

Objective: The cellular protective effects of resveratrol and co-enzyme Q10 supplementation have been reported in different models. However, there is insufficient data on the effects of resveratrol supplementation on oxidative DNA damage and co-enzyme Q10 levels in ovarian ischemia–reperfusion injury. The current study aims to investigate how resveratrol supplementation affects oxidative DNA damage, co-enzyme Q10 levels, inflammatory and proinflammatory indicators, and growth factors in ovarian ischemia– reperfusion injury. Methods: Thirty albino Wistar female rats were divided into 5 groups; control, ovarian ischemia–reperfusion, and ischemia–reperfusion + treatment (25, 50, and 100 mg/kg resveratrol). Hematoxylin–eosin stain was used to evaluate histopathological results. The levels of 8-oxo-2ʹ-deoxy guanosine and co-enzyme Q10 were obtained by enzyme-linked immunosorbent assay. Following RNA isolation, the expression of tumor necrosis factor-α , interleukin-1β, transforming growth factor-β3, hepatocyte growth factor, epidermal growth factor, and vascular endothelial growth factor was assessed. Results: Regarding the histopathological evaluation score, 8-oxo -2ʹ-d eoxyg uanos ine levels, and co-enzyme Q10 levels, there was no statistically significant difference between the ischemia–reperfusion group and the groups that received resveratrol treatment. Tumor necrosis factor-α, interleukin-1β, transforming growth factor-β3, hepatocyte growth factor, epidermal growth factor, and vascular endothelial growth factor expression levels were significantly decreased in the resveratrol -treated groups compared to the ischemia–reperfusion group. Conclusion: The results imply that the positive effects of resveratrol on infla mmato ry-pr oinfl ammat ory cytokine expression may be a major contributor to the anti-oxidant, anti-inflammatory, anti-proliferative, anti-carcinogenic, and therapeutic effects of resveratrol documented in the literature.