Endemik Onosma discedens tohumlarında İn vitro çimlendirme çalışmaları

Onosma discedens is endemic species having considerable narrow distribution in Erzincan (Turkey) Region. In this study, we investigated the germination behavior of Onosma discedens with different concentrations of NaCI, HCl, KNO3, GA3 (100 and 200 ?M) or hot-cold stratification/mechanical scarification. Seeds under the light and dark treatments were exposed to a photoperiod of 8 h light/16 h dark hours with a 23/18 °C thermoperiod. Germination rates increased with KNO3-100 µM (% 12), GA3-100 µM (% 25), GA3-200 µM (% 35), HCI-100 µM (% 9) treatments when compared to the control. The highest speed of germination index was obtained at concentrations of 100-200 µM KNO3. Overall, our results will provide valuable data for ex situ conservation of endemic Onosma discedens.