Araştırmacılar(7)

• Atasever, Aslıhan
• Balcı, Çağrı
• Durmaz, Lokman
• Özyürek, Selçuk
• Sökmen, Teoman Özgür
• Şimşek, Samed
• Yavuz, Emre

• ArticleYalnız künye2027/r/ebyu/10582
  Rosmarinic Acid Attenuates Isoniazid-Induced Neurotoxicity by Restoring Mitochondrial Dynamics and Suppressing Oxidative Stress, Ferroptosis, Neuroinflammation, and Apoptosis

  Bolat, Merve; Karaarslan, Tuba; Teki̇n, Samet; Derelİ, Esra; Atasever, Aslıhan; Laçi̇n, Burak Batuhan; Aktaş Şenocak, Esra; Aykurt, Furkan; Şengül, Emin; Çelebi̇, Fikret

  Despite the continued global burden of tuberculosis and the essential role of isoniazid (INH) in first-line antituberculosis therapy, INH-induced neurotoxicity remains a major clinical challenge that compromises treatment adherence, and effective, well-characterized neuroprotective strategies are still lacking. This study investigated the neuroprotective effects of rosmarinic acid (RA) against INH-induced neurotoxicity, evaluating oxidative stress, inflammation, iron metabolism, energy homeostasis, mitochondrial dynamics, ferroptosis, and apoptosis using biochemical, molecular, and histopathological approaches. INH markedly increased malondialdehyde while reducing superoxide dismutase and glutathione, and increased TNF-α, IL-1β, and IL-6 while decreasing IL-10. INH also disturbed iron homeostasis (increased Fe²⁺), impaired energy metabolism (reduced ATP), and disrupted mitochondrial dynamics (increased Drp-1; decreased Mfn-2 and PGC-1α). NF-κB activation was accompanied by increased ACSL4 and decreased GPX4 and FTH1, reflecting ferroptosis, alongside increased Bax, Caspase-3, and Cytochrome c and reduced Bcl-2, indicating mitochondrial apoptosis. RA treatment suppressed oxidative stress, enhanced antioxidant defense, attenuated inflammation, restored iron and energy homeostasis, improved mitochondrial dynamics, and inhibited NF-κB activation, ferroptosis, and apoptosis. These findings demonstrate that RA exerts potent neuroprotective effects against INH-induced brain injury by simultaneously targeting oxidative stress, mitochondrial dysfunction, iron dyshomeostasis, neuroinflammation, ferroptosis, and apoptosis. To our knowledge, this is among the first studies to comprehensively address these interconnected pathways, including ferroptosis and iron dyshomeostasis, in INH-induced neurotoxicity. RA may thus represent a promising therapeutic candidate for preventing INH-induced neurotoxicity.

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• CorrectionYayıncıda açık erişim2026/r/ebyu/22833
  Correction: p-Coumaric Acid Attenuates Lead Acetate-induced Neurotoxicity in Rats by Improving Behavioral Dysfunction and Suppressing Oxidative Stress, Neuroinflammation, Apoptosis, and Plasticity-related Molecular Alterations

  Karaarslan, T; Bolat, M; Bolat, İ; Orhan, B; Senocak, EA; Tekin, S; Lacin, BB; Golgeli, A; Atasever, A

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• ArticleYayıncıda açık erişim2026/r/ebyu/11495
  p-Coumaric Acid Attenuates Lead Acetate-induced Neurotoxicity in Rats by Improving Behavioral Dysfunction and Suppressing Oxidative Stress, Neuroinflammation, Apoptosis, and Plasticity-related Molecular Alterations

  Karaarslan, Tuba; Bolat, Merve; Bolat, İsmail; Orhan, Betul; Senocak, Esra Aktas; Tekin, Samet; Lacin, Burak Batuhan; Golgeli, Ayse; Atasever, Aslıhan

  This study evaluated the neuroprotective effects of p-coumaric acid (p-CA) against lead acetate (PbAc)-induced neurotoxicity in rats by focusing on oxidative stress, neuroinflammation, apoptosis, and neuronal plasticity. 35 male rats were randomly assigned to five groups: Control, p-CA (100 mg/kg), PbAc (30 mg/kg), PbAc + p-CA (50 mg/kg), and PbAc + p-CA (100 mg/kg). Treatments were administered orally for 14 days. Behavioral performance was assessed using the open field test (OFT) and elevated plus maze (EPM). Biochemical analyses of brain tissue included the measurement of MDA, GSH, SOD, CAT, and GPx levels. Western blotting was performed to determine the protein expression levels of inflammatory, glial, and synaptic markers, including NF-κB, IL-1β, IL-6, TNF-α, Iba-1, GFAP, PSD-95, and AChE. Quantitative real-time PCR was used to assess the mRNA expression levels of Bax, Bcl-2, Caspase-3, MAP-2, NeuN, and GAP-43. Histological evaluations included hematoxylin-eosin (H&E) staining for neuronal morphology, immunohistochemistry (IHC) for 8-OHdG, GABA, and GFAP, and immunofluorescence (IF) staining for BDNF expression. PbAc exposure caused marked oxidative damage, neuroinflammatory activation, enhanced apoptotic signaling, and impaired neuronal plasticity, accompanied by reduced antioxidant defenses and altered expression of plasticity-related markers. Co-treatment with p-CA significantly ameliorated these biochemical, molecular, and histopathological alterations in a dose-dependent manner. The 100 mg/kg dose of p-CA showed the strongest neuroprotective effect, restoring many parameters close to control levels. Overall, these findings suggest that p-coumaric acid exerts neuroprotective effects against PbAc-induced brain injury by attenuating oxidative stress, neuroinflammation, and apoptosis while supporting neuronal plasticity. Therefore, p-CA may represent a promising natural compound for further investigation in heavy metal-induced neurotoxicity models. However, further studies are required to clarify the upstream molecular signaling pathways involved and to determine whether these protective effects can be maintained under long-term exposure conditions.

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• ArticleYalnız künye2026/r/ebyu/11482
  Removal of Malachite Green from Aqueous Solutions by Using Bi4V2O11 Nanosheet

  Yavuz, Emre; Dundar, Ozan Ali; Yavuz, Sevtap Caglar; Yilmaz, Erkan; Ozdokur, Kemal Volkan; Cirak, Burcu Bozkurt; Caglar, Bulent; Cirak, Cagri; Arar, Özgür

  Introduction: Transition metal oxides are highly effective for dye removal due to their adaptable surface chemistry, availability, and robust mechanical and thermal properties. Among them, Bi4V2O11 nanosheets offer potential due to their morphology and high surface area. To synthesize Bi4V2O11 nanosheets and evaluate their performance in removing malachite green, focusing on the impact of adsorbent amount, contact time, and interfering ions on dye removal efficiency. This study aims to evaluate the performance of Bi4V2O11 nanosheets in removing malachite green dye from aqueous solutions and to examine the effects of adsorbent dosage, contact time, and the presence of coexisting ions on the sorption process. Methods: Bi4V2O11 nanosheets were synthesized and used to remove the malachite green dye. Batch experiments were conducted to study the effects of adsorbent dose, contact time, and coexisting ions (Na+, Ca2+, and tannic acid). Equilibrium data were fitted to the Langmuir isotherm model to determine the maximum sorption capacity. Results: Malachite green sorption using Bi4V2O11 nanosheets reached equilibrium within 240 minutes, demonstrating efficient dye removal under the tested conditions. The sorption process conformed to the Langmuir isotherm model, indicating monolayer adsorption with a maximum capacity of 47.27 mg/g. While the presence of Na+ and tannic acid had negligible effects on dye removal efficiency, the coexistence of Ca2+ significantly reduced the removal efficiency from 95% to 58%, highlighting the potential impact of divalent ions on the adsorption process. Discussion: Bi4V2O11 nanosheets are effective adsorbents for malachite green removal, demonstrating high sorption capacity and resilience to Na+ and tannic acid interference. However, the presence of Ca2+ significantly reduces removal efficiency, highlighting the need for further optimization in practical applications. Conclusion: Bi4V2O11 nanosheets offer high sorption capacity and structural stability, environment friendly synthesis procedure, making them feasible for small-scale or pre-treatment applications. 2026, Bentham Science Publishers

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• ArticleYalnız künye2026/r/ebyu/11439
  A new species of the genus Camerotrombidium Thor (Acari: Microtrombidiidae), with a checklist of trombidioid mites of Türkiye

  Balcı, Çağrı; Sevsay, Sevgi

  A new species, Camerotrombidium akınleventi sp. nov. is described based on both active postlarval forms and larvae. This is the third species of Camerotrombidium for which larval stage has been observed under laboratory conditions. Additionally, based on published records and original data, a checklist of trombidioid mites from Türkiye is compiled. https://zoobank.org/References/0448531d-dc2a-4c36-a974-41ac76de44f7.

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• ArticleYalnız künye2026/r/ebyu/11095
  Chemical Composition and Antibacterial Activity of Rosa villosa subsp. mollis R. Keller & Gams., Rosa hemisphaerica J. Herrm. and Natural Hybrid Rosa ozcelikii Korkmaz & Kandemir Essential Oil

  Gözcü, Sefa; Akşit, Zeynep; Akşit, Hüseyin; Şimşek, Samed; Korkmaz, Mustafa

  This study explores the essential oils (EOs) of Rosa hemisphaerica (RH), Rosa villosa subsp. mollis (RV) and the newly identified hybrid Rosa ozcelikii (RO). The major components of the EOs were found to be nonadecane (22.45%), viridiflorol (19.37%) and heneicosane (11.72%) in RH; α-muurolene (14.72%), σ-cadinene (17.88%) and farnesol (9.94%) in RV; and heptacosane (27.63%), heneicosane (12.42%) and α-citronellol (11.90%) in RO. Antibacterial activities were evaluated using the disc diffusion method. The EOs demonstrated moderate antibacterial effects: RV and RH exhibited inhibition zones of 8.0 ± 0.4 mm against Pseudomonas aeruginosa, while RO showed stronger activity with inhibition zones of 11.0 ± 0.5 mm against Staphylococcus aureus, 12.0 ± 0.4 mm against Clostridium perfringens and 9.0 ± 0.5 mm against Bacillus cereus. These findings highlight the chemical diversity and potential therapeutic applications of these Rosa species, emphasising their relevance in the search for natural antimicrobial agents.

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• ArticleYalnız künye2026/r/ebyu/10872
  Cytotoxic and apoptotic effects of Fe, Cu, and Ag nanoparticles synthesized with sage (Salvia officinalis) extract on rainbow trout gonad cell line

  Kırıcı, Muammer; Erdoğan, Mehmet Kadir; Caf, Fatma; Gündoğdu, Ramazan; Taysı, Mehmet Reşit; Kırıcı, Mahinur; Sökmen, Teoman Özgür; Kaya, Bülent; Toy, Yusuf

  The rapid increase in the use of nanoparticles in various sectors necessitates understanding their potential biological effects on aquatic organisms. In particular, the biosafety profiles of plant-derived biosynthetic nanoparticles, developed as environmentally friendly alternatives, are still known to a limited extent. In this study, the cytotoxic and apoptotic effects of iron (Fe), copper (Cu), and silver (Ag) nanoparticles obtained by green synthesis using sage ( Salvia officinalis ) leaf extract on rainbow trout ( Oncorhynchus mykiss ) gonad cell line (RTG-2) were investigated. The structural and morphological properties of the synthesized nanoparticles were characterized by UV–Visible spectroscopy, FT-IR, X-ray diffraction (XRD), scanning electron microscopy (SEM)-EDX, and zeta potential analyses. Cell viability and apoptosis-related protein responses were assessed following nanoparticle exposure. The results showed that all metal nanoparticles produced a concentration-dependent cytotoxic effect in RTG-2 cells. Silver nanoparticles (AgNPs) exhibited the strongest cytotoxic effect, significantly reducing cell viability, especially at concentrations of 50 µg/mL and above. Apoptosis analysis revealed that different toxicity mechanisms were activated depending on the metal type. While AgNPs strongly suppressed BCL2 expression and appeared to enhance apoptosis-related responses, CuNPs and FeNPs were mainly associated with p53-mediated cellular stress signaling. In conclusion, it was shown that sage-derived metal nanoparticles produced metal-type-specific cytotoxic and apoptotic effects in fish gonad cells; these findings highlight the necessity of species- and metal-based approaches in the environmental risk assessment of green synthesis nanoparticles.

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• ArticleAçık Erişim2026/r/ebyu/10865
  Effects of syringic acid on the indomethacin-induced gastric ulcer model in rats: in vivo and in silico study

  ATASEVER, Aslıhan; ÇELEBİ, Fikret; YILDIRIM, Serkan; BOLAT, İsmail; ÇINAR, Burak

  In this study, the potential protective effects of syringic acid (SA) on gastric tissue were investigated in an indomethacin (INDO)-induced gastric ulcer model. A total of 84 male Sprague–Dawley rats were randomly divided into seven groups. In the in vivo experiments, rats were administered SA at doses of 5, 50, and 100 mg/kg and omeprazole (OMP) at a dose of 5 mg/kg intragastrically (i.g.) for 14 days, and indomethacin (100 mg/kg, i.g.) was administered on the final day. Following INDO administration, the rats were sacrificed under anesthesia, and gastric tissues were carefully excised for further analyses. The collected gastric tissues were subjected to biochemical, histopathological, and immunofluorescence analyses. In addition, in silico analyses were performed to support the INDO-induced gastric ulcer model. Using the licensed Schrödinger Maestro 2025/1 software, the binding properties of INDO to the COX-1 receptor were evaluated through molecular docking, MM-GBSA, and pharmacophore matching analyses. INDO administration was associated with oxidative stress, inflammation, apoptosis, and histopathological damage in gastric tissue. SA treatment appeared to alleviate INDO-induced gastric injury through its antioxidant, anti-inflammatory, and anti-apoptotic properties. SA treatment ameliorated histopathological alterations in ulcerated areas, particularly at doses of 50 and 100 mg/kg, whereas the 5 mg/kg dose did not show a significant protective effect. In addition, in silico analyses suggested that INDO may contribute to ulcer formation by inhibiting COX-1, thereby reducing prostaglandin production in the gastric mucosa. Overall, the findings of this study suggest that SA may reduce oxidative stress, suppress inflammatory responses, and inhibit apoptosis, thereby contributing to the protection of gastric tissue against INDO-induced injury. These results indicate that SA may have therapeutic potential for the prevention of NSAID-induced gastric injury; however, further experimental and clinical studies are needed to confirm these effects and clarify the underlying mechanisms.

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• ArticleYayıncıda açık erişim2026/r/ebyu/10842
  Chemical Composition and Biological Activities of Propolis From Eastern Anatolia: Antioxidant, Enzyme Inhibitory, and Antimicrobial Activities

  Fırat, Mehmet Çağlar; Akşit, Zeynep; Şimşek, Samed; Altın, Sevgi; Yıldız, İlyas; Akşit, Hüseyin

  Propolis is recognized for its biological activities; however, its chemical composition varies according to environmental conditions, which presents challenges for quality standardization and biological evaluation. Although propolis has been widely investigated, information regarding variation among samples collected from geographically close locations within the same region is limited. Therefore, this study investigated whether propolis samples obtained from different locations within Erzincan Province exhibit differences in chemical composition and biological activities and evaluated the relationship between environmental conditions and propolis characteristics. Eleven propolis samples collected from Erzincan, Türkiye, were analyzed for phenolic composition, antioxidant capacity, antimicrobial activity, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase inhibitory activities. Twenty-eight compounds were identified, with gallic acid, protocatechuic acid, chlorogenic acid, hydroxybenzaldehyde, caffeic acid, and vanillin detected as major constituents. AChE inhibitory activity varied between 1.28 and 6.44 µg/mL, while BChE inhibitory activity varied between 1.85 and 9.66 µg/mL. All samples exhibited antibacterial activity. Total phenolic and flavonoid contents were 141.70–255.04 mg GAE/g extract and 30.48–93.81 mg QE/g extract, respectively. The KMH sample showed the antioxidant activity. The results demonstrated variation in phytochemical composition and biological activities among propolis samples collected within the same province, indicating associations between flora, environmental conditions, propolis quality, and bioactivity.

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• ArticleYayıncıda açık erişim2026/r/ebyu/10787
  Integrated Analysis of Volatile and Phenolic Profiles and Biological Activities of Satureja hortensis From Erzincan

  Şimşek, Samed

  Satureja hortensis, widely used in traditional medicine and culinary applications, is a valuable aromatic plant rich in essential oils (Eos) and bioactive compounds. This study comprehensively evaluated the phytochemical composition and biological properties of S. hortensis grown in Erzincan, Turkey. The EO and four solvent-based extracts (ethyl acetate, butanol, water extract (WE), and water fraction) were analyzed. GC–MS/MS identified 34 volatile compounds (98.76%), with thymol (40.83%), carvacrol (19.77%), γ-terpinene (16.71%), and p-cymene (12.06%) as major constituents. LC-ESI-MS/MS revealed phenolic compounds, with hesperidin, rutin, and caffeic acid as major constituents in ethyl acetate and butanol fractions. Antioxidant activity was determined by DPPH, ferric reducing antioxidant power (FRAP), TPC, and TFC assays. The ethyl acetate showed the strongest DPPH scavenging (17.25 ± 2.34 μg/mL), highest TPC (79.24 ± 0.59 mg gallic acid equivalent /g), and moderate FRAP activity (96.86 ± 1.06 mg TE/g), while the WE had the highest flavonoid content (48.21 ± 1.56 mg QE/g). The EO exhibited potent antimicrobial activity (MIC 31.25–250 μg/mL; maximum inhibition zones 50 mm) against A. niger, C. albicans, and A. alternata. HPLC-DAD quantified rosmarinic acid, highest in ethyl acetate extract (580.93 ± 1.12 mg/g). Pearson correlation analyses demonstrated strong positive associations between phenolic content and biological activities, supporting the pharmaceutical, cosmetic, and industrial potential of S. hortensis.

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