Gökçe, Yasin; Çıçek, Betül; Danışman, Betül

Amaç: Kanser tedavisinde kullanılan ilaçlar kalp sorunlarına neden olmakta ve bu da kullanımlarını kısıtlamaktadır. Ayrıca, bu ilaçların uygulanma yönteminin farklı sonuçlara neden olduğu görülmektedir. Bu nedenle bu çalışmada, doksorubisin (DOX) ajanının akut ve kronik olarak uygulanmasının kalbin elektriksel aktivitesi üzerinde farklı etkilerinin olup olmadığının incelenmesi amaçlandı. Materyal ve Metod: Ağırlıkları 200-250 gram arasında değişen 26 adet erkek Wistar-Albino sıçan üç gruba ayrıldı: Kontrol grubu: hayvanlara herhangi bir tedavi uygulanmadı (n=8), DOX akut grubu; 3 haftanın sonunda 15,05 mg/kg DOX i.p. olarak verildi (n=10), DOX kronik grubu; 3 haftada yedi kez her defasında 2,15 mg/kg DOX intraperitoneal (i.p.) uygulandı (n=10). Deney süresi sonunda tüm hayvanlardan elektrokardiyogram (EKG) ölçümleri alınarak değerlendirildi. Bulgular: EKG verilerine göre kalp atım hızı, P dalga genliği ve P süresinin akut ve kontrol grupları arasında farklılık göstermediğini ancak kronik grupta istatistiksel olarak anlamlı azaldığı görüldü. Her iki DOX grubunda da PR intervali kontrole göre değişmedi. Ayrıca kronik grupta RR intervali anlamlı derecede artarken akut DOX grubunda değişmedi. Her iki DOX grubunda da QRS süresinin oldukça arttığı görüldü. Ayrıca her iki DOX grubunda da QT aralığında ciddi bir artış olduğu ancak kronik grupta artışın daha belirgin olduğu görüldü. Sonuç: Sonuç olarak bu ilaçların uygulanma şeklinin kalp fonksiyonlarında anlamlı değişikliklere yol açabileceği düşünülmektedir. Akut DOX tedavisi, özellikle P dalgası genliği (atriyal kasılmanın bir ölçüsü) ve P dalga süresi (kasılmanın uzunluğu) üzerinde olumsuz etkilerinin olmayışı ile, kronik maruziyetten daha az zararlı olabileceği düşünülmektedir. Ancak bu bulguları doğrulamak için daha ileri çalışmalara ihtiyaç vardır.

Çıçek, Betül; Danışman, Betül

Background: We aimed to investigate the ability of resveratrol carbon dots (RES C-Dots) to protect SHSY5Y cells from oxido-inflammatory stress and apoptosis caused by 6-hydroxydopamine (6-OHDA). Materials and Methods: In vitro PD model was generated in SH-SY5Y cells by administering of 200 μM 6- OHDA for 24 hours. Different concentrations of RES C-Dots (12.5, 25, and 50 μg/mL) were applied to the cells 30 minutes before administration of 6-OHDA. Results: We observed that application of RES C-Dots prevented cell death induced by 6-OHDA and maintained cell viability. As expected, RES C-Dots prevented oxidative damage induced by 6-OHDA - by strengthening the total amount of antioxidants and lowering the total amount of oxidants in SH-SY5Y cells. Similarly, RES C-Dots markedly alleviated the secretion of inflammatory factors (TNF-α and IL-1β) promoted by 6-OHDA. Furthermore, RES C-Dots prevented apoptosis induced by 6-OHDA by suppressing caspase-3 mRNA expression level. Conclusions: RES C-Dots rescued SH-SY5Y cells from 6-OHDA- induced damage by modulating the oxidoinflammatory and apoptotic response. This report indicates enounces that RES- synthesised C-Dots may have promising curative potential for PD.

Reşat, Mehmet; Durmaz, Riza; Çıkman, Aytekin; Karahocagil, Mustafa Kasim; Karagoz, Alper

Background: With this study, the detection of the resistance rates of A.baumannii strains isolated from the patients in the intensive care units to the antibiotics and the demonstration of the clonal relationship of the isolates with multi-drug resistant of the factor of nosocomial infection were aimed by using the method of pulsed-field gel electrophoresis (PFGE). Materials and Methods: At the Medical Faculty Hospital of Yüzüncü Yıl University, 69 patients followed between January 2010-October 2011 at the Intensive Care Unit of Anesthesiology and Reanimation (ICUAR), Intensive Care Unit of Chest Diseases and Pediatric Intensive Care Unit and July-November 2012 at ICUAR and the same number of isolates formed with A.baumanii, Ventilator Associated Pneumonia (VAP) and diagnosis of Primary Bloodstream Infection (PBI) were enrolled to the study. Results: Of the 69 patients included in the study, 35 were men (50.7%) and 34 (49.3%) were women. The ages of the patients ranged from 1 to 86 years (mean age 54). 63 (91%) of the patients were diagnosed with VAP and 6 (9%) of them with PBI. At least two invasive interference had been performed to the all patients during the staying at the ICU. Urinary catheterization (100%), mechanical ventilation (94.2%) and peripheral venous catheterization (91.3%) were the most frequently performed invasive interferences. Antibiotic susceptibilities to imipenem, colistin and tigecycline were studied with the E-test and to the other antibiotics with the disk diffusion. Colistin was the most effective antibiotic for all of the strains (98.6%). Then, tigecycline (97.1%) and levofloxacin (69.6%) were in the second place. All of the strains were resistant to ceftriaxone, piperacillin/tazobactam, meropenem and imipenem. With the PFGE analysis of A.baumannii isolates performed to the genomic DNA cut with ApaI, it was found that 62 of the 69 A.baumannii strains (89.9%) were within the cluster. These strains were located within 16 clusters. 7 of the strains (10.1%) showed the profiles of specific PFGE and 23 pulsotip were detected among the strains. Conclusion: Being high of clustering rate of multidrug-resistant A. baumannii isolates and isolating the isolates in similar genotypes from the patients in different units is the indicator of these bacteria to be endemic situation in the ICUs of our hospital. If precautions are not taken, A. baumannii has been able to remain in the hospitals for many years and can be easily transmitted among the patients. Especially for the prevention of cross-contamination, the necessity and importance of infection control programs have once again emphasized.