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- ArticleYalnız künye2024Liquid chromatography–tandem mass spectrometry profile and antioxidant, antimicrobial, antiproliferative, and enzyme activities of Thymus pectinatus and Thymus convolutus: in vitro and in silico approach
Akman, Tuğrul Çağrı; Şimşek, Samed; Akşit, Zeynep; Akşit, Hüseyin; Aydin, Ali; Tüfekçi, Ali Riza; Adem, Sevki; Yilmaz, Mustafa Abdullah
BACKGROUND: The objective of this study is to investigate the antiproliferative, antioxidant, antimicrobial, and enzyme activity capacities and phytochemical compositions of Thymus pectinatus (TP), Thymus convolutus (TC), which are endemic to Türkiye. Quantitative analysis of phenolic compounds in the extracts was conducted using liquid chromatography–tandem mass spectrometry, targeting 53 phenolic compounds. RESULTS: Rosmarinic acid, quinic acid, and cynaroside were identified as the major compounds, exhibiting quantitative variation in both extracts. The extracts had a high total phenolic content, with 113.57 ± 0.58 mg gallic acid equivalents (GAE)/g extract for TP and 130.52 ± 1.05 mg GAE/g extract for TC. Furthermore, although both extracts exhibited high total flavonoid content; the TP extract (75.12 ± 1.65 mg quercitin equivalents (QE)/g extract) displayed a higher flavonoid content than the TC extract (30.24 ± 0.74 mg QE/g extract) did. The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5–14.2%) against normal cells. Both extracts exhibited very potent inhibitory activity against the xanthine oxidase enzyme, with half-maximal inhibitory concentration values of respectively 2.07 ± 0.03 μg mL−1 and 2.76 ± 0.06 μg mL−1 and moderate activity against tyrosinase and α-glucosidase. Docking simulations proved that rosmarinic acid and cynaroside, the major components of the extracts, were the most potent inhibitors of xanthine oxidase. According to antimicrobial activity results, the TC extract exhibited moderate activity against Staphylococcus aureus, and the TP extract had strong activity against both Enterococcus faecium and S. aureus. CONCLUSION: These findings emphasize the beneficial effects of the two endemic Thymus species on human health and suggest their potential use as plant-derived bioactive agents.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Phytochemical analysis and biological evaluation of Ferulago setifolia K. Koch
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydın, Ali; Yılmaz, Mustafa Abdullah; Akşit, Hüseyin
BACKGROUND: Ferulago setifolia K. Koch (Apiaceae) has been the subject of this study, aiming to comprehensively determine its phenolic fingerprint and evaluate its various biological activities. The liquid chromatography–tandem mass spectrometry analysis of the 70% methanol extract of F. setifolia (FS) revealed the presence of 23 phytochemicals, among which chlorogenic acid, quinic acid, kaempferol-3-O-glucoside, and quercetin-3-O-glucoside were identified as the major phenolics in the extract. RESULTS: The biological screening included examinations of antioxidant, antibacterial, antiproliferative, and cytotoxic activities. The FS extract displayed moderate 2,2-diphenyl-1-picrylhydrazyl radical scavenging and ferric-reducing capacity, indicating moderate antioxidant activity. Furthermore, FS exhibited significant antiproliferative effects on cancer cells while showing low cytotoxicity on normal cells. The antibacterial activity findings revealed that FS demonstrated potent activity against Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, and Escherichia coli. CONCLUSION: The findings of this study suggest that the methanolic extract of FS holds promise as a potential source of biologically active compounds. It can be utilized for the development of pharmaceutical formulations, thanks to its significant antiproliferative and antibacterial activities. Additionally, FS can serve as a valuable source of chlorogenic acid for industrial applications.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Exploring the potential of bilberry extracts as natural antifungal and bioherbicidal agents in agriculture: composition and antioxidant activity; [Istraživanje potencijala ekstrakta borovnice kao prirodnih antifungalnih i bioherbicidnih agenasa u poljoprivredi: sastav i antioksidativna aktivnost]
Bayar, Yusuf; Yılar, Melih; Akşit, Hüseyin; Genç, Nusret
This study aims to investigate the composition of the leaf essential oil and the total phenolic and flavonoid contents as well as the antioxidant activity of the n-hexane, ethyl acetate, and methanol extracts of Vaccinium myrtillus leaves collected from two different locations (Muğla and Mersin, Turkey). In addition, the antifungal activity against Sclerotinia sclerotiorum (SS), Fusarium oxysporum f sp. melonis (FOM), Fusarium oxysporum f sp. cucumerinum (FOC), and Rhizoctonia solani (RS) and the bioherbicidal activity against Taraxacum officinale and Rumex crispus were evaluated for the first time. The methanolic extract inhibited mycelium growth of SS, FOM, FOC, and RS in a dose-dependent manner. No significant difference was observed in antifungal activity between the two different collection sites. In the bioherbicidal activity tests, the methanol extract of the fruits completely suppressed the root-shoot development of Taraxacum officinale, while also significantly inhibiting the root-shoot development during the seed germination of Rumex crispus compared to the control group at 3 mg/mL concentration for both locations. Based on the results of the GC/MS analysis, the major constituents identified in the leaf essential oils collected from the Muğla and Mersin locations were α-pinene (29.16%/15.75%), eucalyptol (22.19%/26.46%), linalool (12.66%/25.27%), and linalyl acetate (7.43%/2.71%). The total phenolic and flavonoid contents of the plant extracts for the Muğla and Mersin samples were determined as (42.80‒157.58 GAE/g extracts), (29.38‒151.44 GAE/g extracts) and (10.52‒37.88 QE/g extracts), (14.81‒44.18 QE/g extracts) respectively. In addition, the plant exhibited significant antioxidant activity. These findings indicate quantitative differences in the chemical composition of the essential oils between the two geographical locations. These findings are significant as they provide insights for the development of new natural antifungal agents with potential applications in agriculture.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024LC-MS /MS characterization and biological activities of Morina persica L. (Caprifoliaceae)
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydin, Ali; Yilmaz, Mustafa Abdullah; Akşit, Hüseyin
The study aimed to comprehensively identify the phenolic fingerprint of Morina persica L. (Caprifoliaceae) and evaluate its various biological activities. LC-MS/MS analysis of 70% Morina persica methanol extract revealed the presence of 27 phytochemicals, with quinic acid, chlorogenic acid, and rutin being the major phenolics. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities were evaluated for biological screening. The results showed that the methanolic extract of M. persica has a moderate DPPH radical scavenging and ferric-reducing capacity, indicating antioxidant activity. M. persica was observed to have a sufficient antiproliferative effect against cancer cells and low cytotoxicity against normal cells. Moreover, M. persica demonstrated good antibacterial activity against Clostridium perfringens, Enterococcus faecalis, and Escherichia coli. These data suggest that the methanolic extract of M. persica could be considered both an industrial source of quinic acid and a potential biologically active ingredient for developing drug formulations.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Isolation, characterization and anticancer activity of secondary metabolites from Verbascum speciosum
Demirci, Serpil; Alp, Cemalettin; Akşit, Hüseyin; Ulutaş, Yakup; Altay, Ahmet; Yeniçeri, Esma; Köksal, Ekrem; Yaylı, Nurettin
Herein, two iridoid glucosides aucubin (1) and ajugol (2), and two phenyl ethanoids, verbascoside (3) and poliumoside (4) were isolated from the methanol extract of the aerial parts of Verbascum speciosum and used to study about their anticancer activity for the first time. The structures of all compounds were elucidated using spectroscopic data (IR, 1D and 2D NMR, LC-TOF/MS). Antiproliferative activities of Aucubun (1) and Verbascoside (3) were tested against A-549 (human colon cancer), MDA-MD-453 (human breast cancer) and 3T3-L1 (mouse fibroblast)cell lines by XTT assay. In addition, the anticarcer mechanism of action of aucubin (1) was investigated on MDA-MB-453 cells for the first time. XTT result showed that both applied compounds exhibited antiproliferative effect at different dose ranges depending on the cancer type, as well as selectivity between cancer and healty cell lines. Flow cytometry analyzes revealed that aucubin (1) exerts its cytotoxic effect in MDA-MB-453 cells by directing cells to early apoptosis and inhibiting the P13K/AKT signaling pathway.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Isolation of Undescribed Biflavonoid from Potamogeton pusillus L. and Antidiabetic Activity Properties
Akşit, Hüseyin
A phytochemical study on the Potamogeton pusillus L. led to the isolation and identification of a novel biflavonoid, pusillin A (1) with known seven compounds, including rosmarinic acid (2), luteolin-7-sulfate (3), eriodictyol-7-sulfate (4), chrysoeriol-7-sulfate (5), eriodictyol (5), luteolin (7) and chrysoeriol (8). Their structures were elucidated based on spectrometric analysis, including LC-Q-TOF/MS and NMR. All compounds were screened for in vitro antidiabetic activity using the spectrophotometric method. Sulfated flavones (1, 3, 4, and 5) exhibited prominent α-glucosidase inhibitory activity, and compounds 2, 6, 7, and 8 showed moderate inhibitory activities. All isolates were reported for the first time from the plant material.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Isolation, Characterization, and Antiproliferative Activity of Polymethoxy Flavones from Mentha dumetorum
Akşit, Hüseyin; Ozan, Erdem; Erenler, Ramazan
Background: Natural products have been used commonly in the pharmaceutical industry as well as traditional medicine due to their bioactive contents. Objective: The objective of this study is to isolate the polymethoxy flavones (PMFs) and evaluate the antiproliferative activity. Methods: The PMFs were extracted from Mentha dumetorum with boiling hot water and then partitioned with hexane and the extract was subjected to chromatographic techniques such as Sephadex LH-20, silica gel, and preparative TLC, and HPLC to isolate the compounds. The structures of isolated compounds were elucidated by spectroscopic techniques such as1H-NMR,13C-NMR, LC-TOF-MS, and UV-Vis. The antiproliferative effects of isolated compounds were evaluated using a real-time cell analyzer–single plate (RTCA-SP) instrument against HeLa and HT29 cell lines. Results: The isolated PMFs were identified as xanthomicrol (1), 5-hydroxy-6,7,8,4'-tetrametoxy flavanone (2), 5-desmetil sinensetin (3), 5-demetil nobiletin (4), gardenin B (5), 5-hydroxy-6,7,8,3',4'-penta methoxy flavanone (6) and 5-hydroxy-6,7,4'-trimethoxy flavone (7). Compounds 2 and 5 were found to be the most active against both cell lines. Conclusion: The isolated compounds as well as the plant extract of Mentha dumetorum could be promising agents for the drug development process, especially drugs for cancer treatment. Moreover, isolation methods were developed for the corresponding compounds.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Isolation and cytotoxic activities of undescribed iridoid and xanthone glycosides from Centaurium erythraea Rafn. (Gentianaceae)
Akşit, Hüseyin; Gözcü, Sefa; Altay, Ahmet
Centaurium erythraea Rafn. (Gentianaceae) is used in internal traditional therapy as an anthelmintic, hypotensive, antipyretic, and antidiabetic. It is used externally for the treatment of wounds. Ursolic acid, maslinic acid, secologanin, secologanin dimethyl acetal, centauroside A, erythraeaxanthone I, erythraeaxanthone II, and demethyleustomin were isolated from aerial parts of Centaurium erythraea and were identified using spectroscopic methods, including NMR and mass spectrometry. The cytotoxic potency of undescribed compounds was evaluated by the XTT assay against human breast cancer MCF-7, MDA-MB-453 and mouse fibroblast 3T3-L1 cell lines. Erythraeaxanthone II was found to have the most potent cytotoxic activity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Ferruginoside D: A Novel Phenylethanoid from Verbascum leiocarpum
Şimşek, Samed; Akşit, Hüseyin; Aydın, Ali; Köksal, Ekrem
A novel phenylethanoid, ferruginoside D, together with fourteen known compounds were isolated from the Verbascum leiocarpum for the first time. Chemical structures of isolated compounds were determined by spectroscopic analysis including HR-ESI-MS and NMR spectra. The antioxidant, α-glucosidase inhibitory properties, and antiproliferative activities against multiple cell lines (A172, C6, HeLa, A2780, SW620, HT29, Beas2B, RPE, and HSF) of the isolated compounds were evaluated in vitro. According to the results, iridoids, flavonoids (luteolin and apigenin), and phenylethanoids (poliumoside and isoverbascoside) with strong antiproliferative potential were also found to have cytostatic effects. Furthermore, the investigation revealed that compounds luteolin, poliumoside, verbascoside, isoverbascoside, ferruginoside C, ferruginoside D, and ursolic acid show potent alpha-glucosidase inhibitory activity, while compounds luteolin, verbascoside, and isoverbascoside exhibit substantial antioxidant activity. The new compound (ferruginoside D) was found moderately active against cancer cell lines, with strong alpha-glucosidase inhibitory activity, and moderate antioxidant properties.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Design of Thymol Loaded Dental In Situ Gels; In Vitro Evaluation
Gözcü, Sefa; Akşit, Hüseyin; Polat, Heybet Kerem
Objetive: The aim of the study was to develop thymol thermosensitive in situ gels based on poloxamers in order to prolong dental contact time, control drug release, and enhance dental bioavailability. Materials and Methods: Poloxamer 188 (P188) and poloxamer 407 (P407) were used in varying amounts to designed the in situ gels. Mucoadhesive excipient, like hydroxypropyl methylcellulose (HPMC), was transferred to the in situ gels to improve the formulation's ability to adhere to biological surfaces. For the created formulations, in vitro drug release, pH, clarity, and sol-gel transition temperature were all evaluated.Results: The all of in situ gels gelation temperatures of the developed formulations range from 33 to 37°C, pH values are around 7, and syringeability is defined as the amount of force necessary to discharge each formulation from a syringe fitted with a 20-gauge needle. The quantities of P407 and HPMC with the preparations, decreasing in vitro burst release while also increasing the viscosity but every in situ gel formulation releases for six hours. Conclusion:The results show that in situ gels containing P407 and P188 show promise for thymol dental application.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Chemical components and insecticidal effects of essential oils from three lavender cultivars against adult Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae)
Alkan, Mustafa; Güzel, Merve; Akşit, Hüseyin; Bahtiyarca Bağdat, Reyhan; Alkan, Fatma Ruveyda; Evli̇Ce, Emre
The study was conducted to determine the insecticidal and behavioral effect of essential oils (EOs) extracted from three lavender cultivars, Hemus, Raya and Yubileina, against adult stage of Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) under laboratory conditions. The contact, fumigant and repellent effects of plant EOs were investigated and possible germination inhibitory effects of the EOs on wheat germination were evaluated. EOs were extracted from the plant samples using a Neo-Clevenger type apparatus where the plant material is subjected to hydro distillation. The chemical constituents of EOs were detected by gas chromatography-mass spectrometry. The experiments were conducted under laboratory conditions in 2019 and 2020. As a result of the study, it was determined that the fumigant activity changed according to the cultivars. While the LC90 value in the fumigant activity of the EOs of the Hemus was determined as 0.157 µl/ml air, this value was recorded as 0.139 µl/ml air and 0.118 µl/ml air in Raya and Yubileina, respectively. Six h after treatment, the highest repellent activity was 86% at 0.05 µl/cm2 with Yubileina. Main EOs components of each cultivar were: Yubileina, linalool (36.0%), linalyl acetate (24.2%) and lavandulyl acetate (5.86%); Hemus, linalool (28.5%), linalyl acetate (23.1%) and lavandulyl acetate (6.59%); and Raya, linalool (42.5%), linalyl acetate (30.0%) and -terpineol (5.45%). There was no negative effect on the germination of wheat with any of essential oils. These results show that lavender EOs could be useful for the control of S. granarius.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2021Isolation, characterization and antiproliferative activity of secondary metabolites from tanacetum alyssifolium (Bornm.) grierson
Köksal, Ekrem; Ulutaş, Yakup; Şimşek, Samed; Bayraktar, Taha; Altay, Ahmet; Çatır, Mustafa; Demirtaş, İbrahim; Tüfekçi, Ali Rıza; Kandemir, Ali; Alp, Cemalettin
Secondary metabolites of Tanacetum alyssifolium (Bornm.) Grierson (Asteraceae) were investigated for the first time. Thirteen compounds including axillarin (1), Luteolin-7-O-β-glucoside (8) and rutin (13) as flavonoids, fraxetin (7), isofraxidin (9), isofraxidin-7-O-glucoside (11) and fraxidin (12) as coumarins, tatridin A (2), altissin (3), tamirin (4) and tanachin (5) as sesquiterpene lactones and 2,4-dihydroxy-6-methoxy acetophenone (6) and picein (10) as acetophenone derivatives were isolated from the methanol extract of the species. The structures of all isolated compounds were elucidated by 1D and 2D-NMR techniques and by comparing with the literature data. In addition, in vitro cytotoxic activity of all isolated compounds was evaluated against breast cancer MCF-7 cell line by XTT assay. Sesquiterpene lactones; tatridin A (2), tamirin (4) and tanachin (5) were found to be the most cytotoxic molecules against MCF-7 cell line.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Vinca soneri'den İzole Edilen Loganic Asidin Probiyotik Bakteriler Lactobacillus acidophilus ve Lactobacillus rhamnosus'un Yüzey Hidrofobisitesi ve Oto-Agregasyonu Üzerine Etkileri
Celebioglu, Hasan Ufuk; Akşit, Hüseyin; Taş, Recep; Çelebi, Busenur
Loganic asit, çeşitli bitkilerde bulunan ve anti-enflamatuar aktivitelere sahip bir iridoid glikozittir. Bu çalışmada loganik asit, laksatif, idrar söktürücü ve antipiretik etkiler nedeniyle halk hekimliğinde kullanılan Vinca soneri bitkisinden izole edilmiştir. Bir grup fonksiyonel gıda olan probiyotikler, vücuda yeteri miktarlarda alındığında insan sağlığını olumlu yönde etkileyen mikroorganizmalardır. En çok çalışılan ve bilinen probiyotikler Lactobacillus acidophilus ve Lactobacillus rhamnosus bakterileri olmakla beraber bu bakteriler, çeşitli gıdalarda veya farklı formülasyonlarda, ayrıca besin takviyelerinde “starter” olarak kullanılır. Bu çalışmanın amacı, loganik asidin Lactobacillus acidophilus LA-5 ve Lactobacillus rhamnosus GG üzerindeki in vitro etkilerini araştırmaktır. Yüzey hidrofobisitesi ve oto-agregasyon, bir probiyotik bakteri için önemli fonksiyonlardır ve bu çalışmanın sonuçları, loganik asidin, probiyotik fonksiyonları modüle edebileceğini, dolayısıyla, loganik asit ve probiyotik suşların kombinasyonlarının daha iyi bir potansiyele sahip olabileceğini göstermiştir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2020Insight into effects of Axillarin and Verbascoside isolated from Tanacetum alyssifolium and Plantago euphratica on probiotic properties of Lactobacillus acidophilus and Lactobacillus rhamnosus
Turan, Nasip Ümran; Çelebioğlu, Hasan Ufuk; Akşit, Hüseyin; Taş, Recep
Phenolic compounds are found in the seeds, flowers, leaves, stems, branches, and fruits of plants. They have an important place in human nutrition as many fruits and vegetables have phenolic compounds. Recently, many phenolic compounds have been used as functional foods and research of new phenolic compounds that are candidate to become functional foods is ongoing. Probiotics which are beneficial microorganisms, are living microbes that benefit the host when taken in sufficient amounts and they have an important role in human gastrointestinal microbiota. The present study aims to investigate effects of Axillarin and Verbascoside, two phenolic compounds isolated from Tanacetum alyssifolium and Plantago euphratica, respectively, on two very common probiotic bacteria Lactobacillus acidophilus and Lactobacillus rhamnosus. For this, growth kinetics, auto-aggregation, and microbial adhesion to solvents, which are some critical properties of probiotics, were investigated. Axillarin and verbascoside showed significant improvement on growths of Lactobacillus acidophilus LA-5 and Lactobacillus rhamnosus GG depending on the dose. Auto-aggregation properties were enhanced by these phenolic compounds, even though surface hydrophobicities were decreased. In conclusion, the present study indicates that the vital conditions of the probiotics could be modulated by Axillarin and/or Verbascoside, consequently their adhesion and colonization capabilities could be altered.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Insight into Effects of Ipolamiide isolated from Plantago euphratica on Probiotic Properties of Lactobacillus acidophilus and Lactobacillus rhamnosus
Köroğlu, Ebru; Celebioglu, Hasan Ufuk; Akşit, Hüseyin; Taş, Recep
Iridoid glycosides are 2-cyclopentanoid derivatives of terpene origin naturally occuring in the leaves, fruits, seeds, bark, roots of variousplants. They are medically important because used in the treatment of many diseases while normally protect plants from biotic andabiotic attacks. They have anti-microbial, anti-tumor, anti-cardiac and anti-inflammatory effects. Ipolamiide is one of the iridoidglycosides and naturally present in many plants. Ipolamiide is very little known compound in terms of the biological activities.The beneficial microorganisms in the body and the plant secondary metabolites can interact in the human gastrointestinal tract.Probiotics are live microorganisms that have many health benefits by improving microbial balance of the intestines. Among the mostknown and most studied probiotics are Lactobacillus acidophilus and Lactobacillus rhamnosus.The aim of the present study is to investigate the effects of ipolamiide on probiotic bacteria Lactobacillus rhamnosus GG (GG) andLactobacillus acidophilus LA-5 (LA-5). For this purpose, ipolamiide was added to the growth of probiotics in different concentrationsand its effects on bacterial growth kinetics, bacterial surface hydrophobicity (Microbial Adhesion to Solvents - MATS Test) and bacterialaggregation (Auto-Aggregation Test) were investigated.The results showed that ipolamiide did not show any important change in surface hydrophobicity of probiotic bacteria. Dose-dependentincreases in auto-aggregation properties of the LA-5 and GG were observed. However, further detailed studies are required to giveinsight into other possible biological activities of ipolamiide.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Arbutin’in yeni kaynakları: Onobrychis nitida and Onobrychis galegifolia
Özdokur, Kemal Volkan; Akşit, Hüseyin; Köksal, Ekrem; Şimşek, Samed
Arbutin (4-Hidroksifenil-β-D-glukopiranozit), hidrokinonun mono glikozidik formudur ve cilt beyazlatma ve çil giderme özellikleri nedeniyle kozmetiklerde uzun süredir yaygın olarak kullanılmaktadır. Bu çalışmada; arbutin flash kromatografi kullanılarak Onobrychis galegifolia'nın metanol ekstraktından izole edilmiştir. Arbutin yapısı, 1 H ve 13C -NMR spektroskopisi ve ayrıca 2D NMR teknikleri ile aydınlatıldı. Ek olarak, O. galegifolia ve O. nitida türlerinde bulunan arbutinin kantitatif analizi HPLC-DAD kullanılarak yapıldı. Sonuçlara göre, O. galegifolia ve O. nitida'nın metanol ekstraktlarının arbutin içeriği sırasıyla 212.02 ve 196.46 mg/g ekstrakt olarak bulundu. Ayrıca, O. galegifolia ve O. nitida'nın soğuk su ekstraktlarının arbutin içeriği 128.2 ve 99.40 mg/g ekstrakt olarak bulundu. Kuru ağırlıklardaki arbutin miktarı, yaklaşık% 5.66 ila% 6.86 arasında hesaplandı.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography
Akşit, Hüseyin; Demirtas, Ibrahim; Keçeci, Mehmet; Genç, Nusret
In this study; it was aimed at the isolation of rosmarinic acid (RosA) from Ocimum basilicum (OB) with a selective extraction and rapid isolation technique using flash chromatography (FC). For this purpose, dried leaves of OB were extracted with n-hexane, dichloromethane, and methanol, respectively. The methanol extract (ME) was extracted with dichloromethane and ethyl acetate using separation funnel by suspending with water. The ethyl acetate layer was dried with anhydrous Na2SO4 and the solvent was evaporated to give a rosmarinic acid-rich extract. Then the ethyl acetate extract (EAE) was separated over silica gel column using FC, and thus RosA was isolated with high purity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Chemical diversity of wild fennel populations from Turkey
Telci, İsa; Dirican, Ali; Elmastas, Mahfuz; Akşit, Hüseyin; Demirtas, Ibrahim
Variation in Essential oil compositions of wild fennel samples from Tokat, Turkey (Foeniculum vulgare Mill.) were studied in the research. Essential oil compositions of 37 fennel full maturated fruit samples from different localities were investigated by GC and GC[sbnd]MS. As results of the analyses, 17 components were identified, and estragole, a phenylpropanoid and isomers of trans-anethole, was major components in all samples following by fenchone. Although they have the same main component in all samples, Quantitative differences of main components caused chemical diversity as a result of cluster analysis. Two chemtypes, estragole with two sub-group and estragole/fenchone, were characterized in the research. Samples having high contents of major components with estragole and fenchone were selected for future studies.
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