Sürdürülebilir Kalkınma Amaçları
Yayınlar
- Review ArticleAçık Erişim2026Phenolic Compounds Found in Citrus Species
Akşit, Zeynep; Yenice, Fatma
Citrus fruits contain many components that are beneficial for health, including minerals, vitamins and dietary fibers. They are also the main source of many bioactive compounds with disease-preventing properties, such as flavonoids and limonoids. Flavonoids are a group of pigments found in plants and are responsible for the coloring of flowers and fruits. The richest source of flavonoids in food is fruits and vegetables. The inedible parts of citrus fruits, such as the juice and pulp of the fruit, are as rich in phenolic compounds as the edible parts of the fruit. Citrus peels and seeds are very rich in phenolic acids and flavonoids, and the peels contain more flavonoids than the seeds. The waste parts of citrus fruits, such as peels and seeds, are especially rich in flavonoids and phenolic compounds, and these compounds have important properties for health. Recycling the millions of tons of citrus waste released worldwide has significant potential in environmental and economic terms. Recovery of secondary metabolites from peels contributes to the prevention of chronic diseases as a natural antioxidant source, while reducing oxidation in foods and has the potential to extend shelf life. The phenolic and flavonoid content of citrus waste varies depending on the species, growing conditions and harvest time. The use of citrus waste in food, cosmetics and pharmaceutical industries not only reduces environmental pollution but also enables the development of value-added products in terms of health and nutrition.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Chemical Composition and Antibacterial Activity of the Essential Oil from Cupressus arizonica Greene Cones Against Clinical and Foodborne
Gözcü, Sefa; Akşit, Zeynep; Dursun, Ahmet
Objective: The purpose of this study was to characterize the chemical composition of the essential oil (EO) derived from the cones of Cupressus arizonica Greene collected from Erzincan and to evaluate its antibacterial potential against a range of pathogenic microorganisms, including strains not previously investigated for this species. Methods: The essential oil was obtained from fresh cones via hydrodistillation for 4 hours. Chemical profiling was performed using Gas Chromatography-Mass Spectrometry (GC-MS). The antibacterial activity was assessed against six pathogenic bacteria using the disc diffusion method, with imipenem serving as a positive control. Results: GC-MS analysis identified 35 compounds, representing 93.83% of the total oil. The major components were α -pinene (53.02%), 3-carene (13.31%), α -myrcene (4.54%), sabinene (4.24%), and limonene (3.47%). The oil exhibited selective antibacterial activity, with inhibition zones recorded for Salmonella enterica (10.0 ± 0.3 mm), Clostridium perfringens (8.1 ± 0.5 mm), and Bacillus cereus (8.0 ± 0.6 mm). However, no inhibitory effect was observed against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Conclusion: This study identifies a distinct α-pinene/3-carene chemotype of C. arizonica influenced by the regional flora of Erzincan. Notably, this research represents the first report of the inhibitory efficacy of C. arizonica cone essential oil against the anaerobic pathogen Clostridium perfringens. The findings suggest that this essential oil could be a promising natural candidate for targeted applications against certain foodborne and anaerobic pathogens.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Chemical Composition and Antibacterial Activity of Rosa villosa subsp. mollis R. Keller & Gams., Rosa hemisphaerica J. Herrm. and Natural Hybrid Rosa ozcelikii Korkmaz & Kandemir Essential Oil
Gözcü, Sefa; Akşit, Zeynep; Akşit, Hüseyin; Şimşek, Samed; Korkmaz, Mustafa
This study explores the essential oils (EOs) of Rosa hemisphaerica (RH), Rosa villosa subsp. mollis (RV) and the newly identified hybrid Rosa ozcelikii (RO). The major components of the EOs were found to be nonadecane (22.45%), viridiflorol (19.37%) and heneicosane (11.72%) in RH; α-muurolene (14.72%), σ-cadinene (17.88%) and farnesol (9.94%) in RV; and heptacosane (27.63%), heneicosane (12.42%) and α-citronellol (11.90%) in RO. Antibacterial activities were evaluated using the disc diffusion method. The EOs demonstrated moderate antibacterial effects: RV and RH exhibited inhibition zones of 8.0 ± 0.4 mm against Pseudomonas aeruginosa, while RO showed stronger activity with inhibition zones of 11.0 ± 0.5 mm against Staphylococcus aureus, 12.0 ± 0.4 mm against Clostridium perfringens and 9.0 ± 0.5 mm against Bacillus cereus. These findings highlight the chemical diversity and potential therapeutic applications of these Rosa species, emphasising their relevance in the search for natural antimicrobial agents.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Chemical Composition and Biological Activities of Propolis From Eastern Anatolia: Antioxidant, Enzyme Inhibitory, and Antimicrobial Activities
Fırat, Mehmet Çağlar; Akşit, Zeynep; Şimşek, Samed; Altın, Sevgi; Yıldız, İlyas; Akşit, Hüseyin
Propolis is recognized for its biological activities; however, its chemical composition varies according to environmental conditions, which presents challenges for quality standardization and biological evaluation. Although propolis has been widely investigated, information regarding variation among samples collected from geographically close locations within the same region is limited. Therefore, this study investigated whether propolis samples obtained from different locations within Erzincan Province exhibit differences in chemical composition and biological activities and evaluated the relationship between environmental conditions and propolis characteristics. Eleven propolis samples collected from Erzincan, Türkiye, were analyzed for phenolic composition, antioxidant capacity, antimicrobial activity, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and α-glucosidase inhibitory activities. Twenty-eight compounds were identified, with gallic acid, protocatechuic acid, chlorogenic acid, hydroxybenzaldehyde, caffeic acid, and vanillin detected as major constituents. AChE inhibitory activity varied between 1.28 and 6.44 µg/mL, while BChE inhibitory activity varied between 1.85 and 9.66 µg/mL. All samples exhibited antibacterial activity. Total phenolic and flavonoid contents were 141.70–255.04 mg GAE/g extract and 30.48–93.81 mg QE/g extract, respectively. The KMH sample showed the antioxidant activity. The results demonstrated variation in phytochemical composition and biological activities among propolis samples collected within the same province, indicating associations between flora, environmental conditions, propolis quality, and bioactivity.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Chemical Composition and Biological Activities of Malus domestica Burkh. ‘Ak Sakı’ Cultivar Growing in Erzincan
Akşit, Hüseyin; Aydemir, Emircan; Şimşek, Samed; Akşit, Zeynep; Çetinkaya, Büşra Demir; Kayır, Ömer
The chemical composition and biological activities of Malus domestica ‘Ak Sakı’ fruits cultivated in Erzincan, Türkiye, were analyzed. The aroma profile of the fruits was investigated for the first time. This study aimed to identify the major volatile and phenolic compounds and determine their antioxidant, antimicrobial, and cytotoxicity properties to investigate the potential health benefits of this cultivar. GC-MS analysis identified major volatile compounds, including 1-hexanol (20.54%), benzaldehyde (13.49%), and 1-octanol (6.68%). LC-MS/MS analysis of phenolic compounds in the ethyl acetate fraction revealed high levels of chlorogenic acid (12 802.67 µg/g), catechin (8601.52 µg/g), and quercetin (805.96 µg/g). Among the tested extracts, the ethyl acetate extract exhibited the highest antioxidant activity (47.18 ± 0.92 µg/mL), total phenolic content (70.17 ± 0.28 mg GAE/g extract), and reducing power (90.17 ± 0.71 mg TE/g extract), followed by butanol and diethyl ether extracts. Antimicrobial tests demonstrated varying inhibition zones, with volatile oil being the most effective. In addition, extracts exhibit cytotoxicity potential. The diethyl ether fraction shows the most potent selective cytotoxicity against A549 lung cancer cells. The findings indicate that the ‘Ak Saki’ cultivar contains diverse bioactive compounds with strong antioxidant, cytotoxicity, and antimicrobial properties. This fruit may contribute to the nutritional and health value of the daily diet.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Comprehensive phenolic profiling and biological evaluation of Centaurea glastifolia L. (Asteraceae)
Gözcü, Sefa; Akşit, Zeynep; Aydın, Ali; Yılmaz, Mustafa Abdullah; Şimşek, Samed
The present investigation focused on the comprehensive analysis of the phenolic profile of Centaurea glastifolia L. (Asteraceae) and the assessment of its diverse biological activities. Utilising LC-MS/MS, the phytochemical composition of the 70% methanol extract of Centaurea glastifolia (CG-ME) was thoroughly elucidated, revealing the presence of 30 distinct phytochemical compounds. Notably, major phenolic constituents identified in the extract included quinic acid, chlorogenic acid, luteolin-7-O-glucoside, kaempferol-3-O-glucoside, luteolin, and apigenin-7-O-glucoside. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities of CG-ME were investigated. The CG-ME exhibited a moderate capacity for scavenging DPPH radicals (IC50: 50.05 ± 1.58 µg/mL) and FRAP (63.96 ± 0.39 mg TE/g extract), indicating a moderate level of antioxidant activity. Moreover, CG-ME demonstrated significant antiproliferative effects (GI50: 1.10 and 1.30 µg/mL) on cancer cells (C6 and HTC cancer cell lines, respectively) while displaying low cytotoxicity towards normal cells (LC50: >1000 µg/mL). In terms of antibacterial activity, CG-ME was found to be inactive against tested both Gram-positive and Gram-negative bacterial strains (MIC > 500 µg/mL). The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5–14.2%) against normal cells.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Antioxidant and antimicrobial activities of plant flowers growing in distinct geological habitats (serpentine, gypsum, and limestone)
Şimşek, Samed; Varol, Tuğçe; Akşit, Zeynep; Elveren, Müjgan; Osma, Etem
In this study, the flowers of 20 plant species [Gypsum (Linum flavum, Paracaryum stenolophum, Onobrychis nitida, Gypsophila lepidioides, Psephellus recepii, Tanacetum heterotomum, Verbascum alyssifolium, limestone (Teucrium leucophyllum, Stachys sparsipilosa, Phlomis oppositiflora, Pelargonium endlicherianum, Chrysophthalmum montanum, serpentine (Convolvulus pseudoscammania, Fumana aciphylla, Gladiolus halophilus, Hypericum thymbrifolium, Salvia indica, Artedia squamata, Erysimum pulchellum, Consolida olopetala], including 11 endemic species, growing in various habitats (gypsum, limestone, and serpentine) in Erzincan province were examined. By focusing on the antioxidant capacities and antibacterial activities of these species, the study aims to elucidate the effects of these ecosystems on the biosynthesis of plant secondary metabolites, primarily phenolic compounds and flavonoids, and their adaptive biochemical responses to unique environmental conditions. Antioxidant properties were assessed using DPPH, FRAP, TPC, and TFC assays. Results showed significant differences in antioxidant compound concentrations among plants from different habitats, with the highest levels in serpentine and gypsum regions. Artedia squamata L. from serpentine had the strongest DPPH activity (24.83 ± 1.25 µg/ml), Erysimum pulchellum had the highest TPC (89.44 ± 0.76 mg GAE/g), and Tanacetum heterotomum had the highest TFC (43.48 ± 2.08 mg QE/g) in gypsum. FRAP activity was highest in serpentine species, particularly Consolida olopetala (32.14 ± 1.84 mg TE/g). Furthermore, Antibacterial assays against eight ATTC strains revealed significant inhibitory effects by Stachys sparsipilosa, Tanacetum heterotomum, Teucrium leucophyllum, and Verbascum alyssifolium against Enterococcus faecium and Salmonella enteritidis with Teucrium leucophyllum showing the highest activity (MIC of 31.25 µg/ml against Salmonella enteritidis). The study highlights the influence of habitat on the biosynthesis of bioactive compounds, suggesting that plants from serpentine and gypsum soils have enhanced antioxidant and antimicrobial properties, making them potential candidates for natural therapeutic agents.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Chemical composition and antimicrobial activity of Scrophularia catariifolia Boiss. & Heldr essential oil
Akşit, Zeynep
In this study, the essential oils of the basal leaves (blEOs) and peduncles (pEOs) -stalks bearing flowers- of Scrophularia catariifolia were separately analyzed using GC-MS for the first time. The main components of two distinctive parts were determined as 4-hexen-1-ol (8.03/6.34%), 1-hexanol (5.48/9.15%), 3-octenol (16.94/6.83%), benzene ethanol (17.21/9.82%), and phytol (15.18/28.68%) for basal leaves and peduncles, respectively. The essential oils showed remarkable antimicrobial activity against the four food-borne pathogens tested. Pseudomonas aeruginosa, Clostridium perfringens, and Bacillus cereus were found to be more susceptible to peduncle essential oil. Two essential oils showed similar activity against Staphylococcus aureus. The essential oils were found to be inactive against Escherichia coli. These findings highlight the potential of S. catariifolia essential oils as natural antimicrobial agents for food safety and preservation. Further investigations are needed for their key applications.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Chemical Composition and Antimicrobial Activity of Hesperis isatidea (Boiss.) D.A.German & Al-Shehbaz Essential Oil
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Akşit, Hüseyin
The essential oils of aerial parts of Hesperis isatidea were analyzed using gas chromatography-mass spectrometry for the first time in the present study. The major components were determined as benzyl salicylate (13.40%), phytol (9.66%), benzyl benzoate (8.57%), undecanal (8.45%), and linalool (5.71%). The essential oil exhibited notable antibacterial activity against Clostridium perfringens, with a minimum inhibitory concentration (MIC) of 125 µg/mL, and Escherichia coli, with an MIC of 62.5 µg/mL. However, it showed limited effectiveness against Staphylococcus aureus and Listeria monocytogenes, both with an MIC of 250 µg/mL, and no antibacterial activity against Enterococcus faecalis and Bacillus cereus. The results demonstrate that the essential oil possesses selective antibacterial properties, especially against specific Gram-negative bacteria, indicating its potential as a valuable complementary antimicrobial agent.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Antioxidant Activity and Theoretical Profile of Novel 2,4,6-Triarylpyridine Derivatives Based on Syringaldehyde
Akşit, Hüseyin; Şimşek, Samed; Akşit, Zeynep; Atahan, Alparslan; Musatat, Ahmad Badreddin; Albayrak, Esra Nur
Eight novel 2,4,6-triarylpyridines derived from syringaldehyde were designed and synthesized via a multicomponent one-step preparation method. Those compounds were evaluated for their antioxidant activities comparing with known reference compounds. In the studied series, toluyl, methoxyphenyl, and pyridinyl-bearing derivatives exhibited considerable antioxidant activities. Meanwhile, using the B3LYP theory and SVP, TZVP basis sets, a comprehensive theoretical quantum computing approach for the synthesized compounds was established and electronic structure descriptive parameters called Fukui indices describing the radical scavenging potential were determined. Finally, the structure-activity relationship was revealed by comparing the theoretical and experimental outputs. As a result, the potential antioxidant activity of the studied compounds was also supported by a theoretical approach.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Antimicrobial Activity and Chemical Composition of Essential Oil Extracted from Hyoscyamus niger L. Inflorescence
Gözcü, Sefa; Akşit, Hüseyin; Akşit, Zeynep
This study aimed to analyze the chemical composition and evaluate the antibacterial activity of essential oils extracted from the H. niger inflorescence collected from Erzincan, Türkiye. The essential oils were extracted using hydrodistillation and analyzed by GC-MS, identifying 23 components, with major constituents being 10-heneicosene (35.72%), phytol (20.50%), and acetic acid, butyl ester (10.10%). The antibacterial activity was tested against six bacterial strains, including Staphylococcus aureus, Clostridium perfringens, Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, and Escherichia coli, using the disc diffusion method. The H. niger inflorescence essential oil exhibited moderate antibacterial activity, specifically against Clostridium perfringens, with a zone of inhibition of 11.7 ± 1.2 mm compared to the positive control imipenem (17.0±1.0 mm). The results suggest that H. niger inflorescence essential oil has potential as an antibacterial agent, highlighting the importance of exploring plant-derived compounds as alternatives to traditional antibiotics in combating multidrug-resistant bacteria.
RDL Kalıcı Kaydıİncele - CorrectionYalnız künye2024Psephellus huber-Marathi (Wagenitz) Wagenitz'in Potansiyelini Keşfetme: Fitokimyasal Bileşiminin Kapsamlı Bir UHPLC-MS/MS Analizi ve Antioksidan, Antimikrobiyal ve Antiproliferatif Aktivitelerinin Değerlendirmesi
Yilmaz, Mustafa Abdullah; Şimşek, Samed; Akşit, Zeynep; Akman, Tugrul Cagri; Aydin, Ali
Psephellus huber-morathii (PH), Doğu Anadolu Bölgesi'nde endemik bir türdür. Bu çalışmada PH'nin %70 metanol ekstraktının biyolojik aktivitelerinin yanı sıra kapsamlı fitokimyasal bileşimi de incelenmiştir. PH ekstraktının fenolik bileşimi, UHPLC-MS/MS yöntemi kullanılarak analiz edildi. Antimikrobiyal özelliklerini değerlendirmek için mikrodilüsyon tekniği kullanıldı. Ekstrenin antioksidan özellikleri, toplam fenolik ve flavonoit içerikleri spektrofotometre kullanılarak belirlendi. Ek olarak, ekstraktların hücre proliferasyonu ve NCI-60 sağkalım parametre değerleri üzerindeki etkileri, MTT deneyi kullanılarak değerlendirildi. Kinik asit ve klorojenik asit majör bileşiklerdi. Ekstrakt, DPPH (IC50:13.9±0.4 μg mL-1) ve FRAP (61.3±2.3 mg TE g ekstrakt-1) yöntemlerinde yüksek antioksidan aktivite göstermiştir. Ekstraktın toplam fenolik ve flavonoid içerikleri sırasıyla 52.2±1.9 mg GAE g ekstrakt-1 ve 28.6±0.9 mg QE g ekstrakt-1 olarak bulundu. Ekstrakt, normal akciğer ve retina hücre hatlarına karşı düşük antiproliferatif aktivite, jinokolojik ve kolon kanser hücreleri üzerinde umut verici bir antikanser etki ve Bacillus cereus ve Enterococcus faecium'a karşı orta derecede antimikrobiyal aktivite gösterdi. Çalışma, PH ekstraktının bulaşıcı hastalıklara ve kansere karşı tıbbi potansiyelini ve değerini göstermiştir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Contribution of Some Agro-Food Processing By-products to Chicken Sausages
Gençcelep, Hüseyin; Akşit, Zeynep
In this study, some agro-food processing by-products were evaluated as novel food ingredients, that meet the consumer's demand for natural ingredients, and their contributions to chicken-type sausage production were examined. Sausages were formulated with 3% quince waste (QS3), 3% grapefruit waste (GS3), 2% tomato waste (TS2), and 3% tomato waste (TS3). Other ingredients were fresh breast chicken meat, beef tallow, spice mix, ice, NaCl, and NaNO2. Proximate composition and sensory analyses were conducted before the storage. Water activity, pH, TBARS, purge accumulation, and microbiological analyses were performed during the storage. QS3, TS2, and TS3 treatments got appreciated (7.0-7.4 out of 10) sensory scores, while GS3 scored low acceptability (4.0 out of 10) points. Adding food waste significantly reduced purge accumulation; the average purge accumulation of the control sample was 3.70% which is approximately two times higher than food waste added samples. The average TBARS value of the control sample was determined as 0.31 mg MA/kg, and the food waste-added samples were found between 0.57-0.65 mg MA/kg during storage. Total mesophilic aerobic bacteria and yeast-mold load were higher in TS2 and TS3 products; microbial load and TBARS values of food waste affected the product quality. Also, water activity, microbial growth, and purge accumulation amount affected each other during storage.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Chemical composition, antifungal, antibacterial, and insecticidal activity of Echinophora chrysantha essential oil
Akşit, Zeynep; Fırat, Mehmet Çağlar; Akşit, Hüseyin; Bayar, Yusuf; Alkan, Mustafa; Şimşek, Samed
This study presents an investigation of the chemical composition and biological activities of the essential oil (EO) extracted from aerial parts of Echinophora chrysantha (EC). Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of twenty-two components, representing 97.5% of the total oil. Major constituents were determined as α-phellandrene (55.74%), β-phellandrene (17.41%), and p-cymene (8.95%). The EO was screened for antimicrobial and antifungal activity against a panel of bacteria and fungi using a broth micro-dilution and fumigant activity assay, respectively. EO sample exhibited better activity against Listeria monocytogenes, Staphylococcus aureus Enterococcus faecium with 31.25, 31.25, and 62.5 μg/mL MIC values. EOs showed moderate mycelium growth inhibitory activity against Phytophthora infestans (32.9±1.9%), Verticillium dahliae (42.3±1.8%), and Fusarium oxysporum f. sp. lycopersici (53.0±2.9%) at 8 μL/petri concentration. The insecticidal activity of EO was also evaluated against two insects (Rhyzopertha dominica and Tribolium confusum) showing moderate activity with mortality rates of 25.8±0.6% and 37.9±1.2% at a 5% (v/v) concentration. Literature search has not revealed any previous work on the antimicrobial, antifungal, and insecticidal activities of the EO from E. chrysantha. In conclusion, this is the first report describing the biological activities of this plant EOs. Additionally, these findings highlight the practical implications of the utilizing essential oil of EC as a potential natural remedy against a spectrum of microbial threats in both pharmaceutical and food preservation applications.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Liquid chromatography–tandem mass spectrometry profile and antioxidant, antimicrobial, antiproliferative, and enzyme activities of Thymus pectinatus and Thymus convolutus: in vitro and in silico approach
Akman, Tuğrul Çağrı; Şimşek, Samed; Akşit, Zeynep; Akşit, Hüseyin; Aydin, Ali; Tüfekçi, Ali Riza; Adem, Sevki; Yilmaz, Mustafa Abdullah
BACKGROUND: The objective of this study is to investigate the antiproliferative, antioxidant, antimicrobial, and enzyme activity capacities and phytochemical compositions of Thymus pectinatus (TP), Thymus convolutus (TC), which are endemic to Türkiye. Quantitative analysis of phenolic compounds in the extracts was conducted using liquid chromatography–tandem mass spectrometry, targeting 53 phenolic compounds. RESULTS: Rosmarinic acid, quinic acid, and cynaroside were identified as the major compounds, exhibiting quantitative variation in both extracts. The extracts had a high total phenolic content, with 113.57 ± 0.58 mg gallic acid equivalents (GAE)/g extract for TP and 130.52 ± 1.05 mg GAE/g extract for TC. Furthermore, although both extracts exhibited high total flavonoid content; the TP extract (75.12 ± 1.65 mg quercitin equivalents (QE)/g extract) displayed a higher flavonoid content than the TC extract (30.24 ± 0.74 mg QE/g extract) did. The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5–14.2%) against normal cells. Both extracts exhibited very potent inhibitory activity against the xanthine oxidase enzyme, with half-maximal inhibitory concentration values of respectively 2.07 ± 0.03 μg mL−1 and 2.76 ± 0.06 μg mL−1 and moderate activity against tyrosinase and α-glucosidase. Docking simulations proved that rosmarinic acid and cynaroside, the major components of the extracts, were the most potent inhibitors of xanthine oxidase. According to antimicrobial activity results, the TC extract exhibited moderate activity against Staphylococcus aureus, and the TP extract had strong activity against both Enterococcus faecium and S. aureus. CONCLUSION: These findings emphasize the beneficial effects of the two endemic Thymus species on human health and suggest their potential use as plant-derived bioactive agents.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Phytochemical analysis and biological evaluation of Ferulago setifolia K. Koch
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydın, Ali; Yılmaz, Mustafa Abdullah; Akşit, Hüseyin
BACKGROUND: Ferulago setifolia K. Koch (Apiaceae) has been the subject of this study, aiming to comprehensively determine its phenolic fingerprint and evaluate its various biological activities. The liquid chromatography–tandem mass spectrometry analysis of the 70% methanol extract of F. setifolia (FS) revealed the presence of 23 phytochemicals, among which chlorogenic acid, quinic acid, kaempferol-3-O-glucoside, and quercetin-3-O-glucoside were identified as the major phenolics in the extract. RESULTS: The biological screening included examinations of antioxidant, antibacterial, antiproliferative, and cytotoxic activities. The FS extract displayed moderate 2,2-diphenyl-1-picrylhydrazyl radical scavenging and ferric-reducing capacity, indicating moderate antioxidant activity. Furthermore, FS exhibited significant antiproliferative effects on cancer cells while showing low cytotoxicity on normal cells. The antibacterial activity findings revealed that FS demonstrated potent activity against Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, and Escherichia coli. CONCLUSION: The findings of this study suggest that the methanolic extract of FS holds promise as a potential source of biologically active compounds. It can be utilized for the development of pharmaceutical formulations, thanks to its significant antiproliferative and antibacterial activities. Additionally, FS can serve as a valuable source of chlorogenic acid for industrial applications.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024LC-MS /MS characterization and biological activities of Morina persica L. (Caprifoliaceae)
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydin, Ali; Yilmaz, Mustafa Abdullah; Akşit, Hüseyin
The study aimed to comprehensively identify the phenolic fingerprint of Morina persica L. (Caprifoliaceae) and evaluate its various biological activities. LC-MS/MS analysis of 70% Morina persica methanol extract revealed the presence of 27 phytochemicals, with quinic acid, chlorogenic acid, and rutin being the major phenolics. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities were evaluated for biological screening. The results showed that the methanolic extract of M. persica has a moderate DPPH radical scavenging and ferric-reducing capacity, indicating antioxidant activity. M. persica was observed to have a sufficient antiproliferative effect against cancer cells and low cytotoxicity against normal cells. Moreover, M. persica demonstrated good antibacterial activity against Clostridium perfringens, Enterococcus faecalis, and Escherichia coli. These data suggest that the methanolic extract of M. persica could be considered both an industrial source of quinic acid and a potential biologically active ingredient for developing drug formulations.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023CHEMICAL COMPOSITION AND ANTIFUNGAL ACTIVITY OF Origanum acutidens ESSENTIAL OIL AGAINST Sclerotinia sclerotiorum (Lib.) DE BARY AND Phytophthora infestans (Mont.) DE BARY
Bayar, Yusuf; Şimşek, Samed; Akşit, Zeynep; Kandemir, Ali
The aim of this study was to investigate the chemical components and evaluate the antifungal activity of Origanum acutidens essential oil. The aerial parts of O. acutidens were collected, and the hydrodistillation method was used to extract the essential oil. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to determine the chemical composition of the essential oil. The main components identified were α-terpineol (4.76%), p-cymene (7.6%), linalool (14.82%), and carvacrol (49.4%). The essential oils were tested against two pathogens in vitro experiments to evaluate their antifungal activity. Different concentrations of the essential oil were applied, and the inhibition of mycelial growth was measured. The results demonstrated that the essential oil exhibited antifungal properties against both pathogens. At a dose of 4.8 µL/Petri dish, the mycelial growth of both pathogens was completely inhibited. However, Sclerotinia sclerotiorum showed higher tolerance to the essential oil compared to Phytophthora infestans. Furthermore, a dose-effect study was conducted as a part of this research. The LC50 values (lethal concentration at which 50% of the pathogens' growth is inhibited) for P. infestans and S. sclerotiorum were calculated as 0.982 µL/Petri and 1.61 µL/Petri, respectively. The study concluded that the essential oil of O. acutidens has the potential to be a natural antifungal agent, particularly against S. sclerotiorum and P. infestans. However, further research is needed to investigate the mechanisms of action and explore potential applications of this essential oil in managing plant diseases.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2023Using Grapefruit and Tomato Waste in O/W Type Chicken Meat Emulsion
Akşit, Zeynep; Gençcelep, Hüseyin
Utilizing food waste is crucial for several reasons, including preserving valuable ingredients, lowering formulation costs, taking advantage of its functional properties, and preserving the environment. Grapefruit waste (GW) and Tomato waste (TW) are used in oil-in-water (O/W) emulsion formulation to investigate their contributions to emulsion stability, textural parameters, and steady shear rheological properties of the model system. O/W emulsions were formed with four different concentrations (0.5%, 1%, 2%, and 3%) of GW and TW, and chicken breast meat and corn oil were the main components of emulsions. Both food wastes increased emulsion stability (ES) in most concentrations compared to the control. The best ES of 86.35 was observed at a concentration of 1% TW emulsion. Emulsions were subjected to texture profile analysis as both row and heat-treated. Heat-treated emulsions had higher hardness values (241.2-518.6 g) than the row emulsions (149.7-247.2 g). All emulsions were found to have pseudoplastic character and exhibit shear thinning behavior. All emulsion samples' apparent viscosity fit well with the Ostwald-de-Waele model (R2>0.9). Food waste samples used in the study improved the chicken-type O/W emulsion properties in terms of emulsion stability and textural properties.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Effects of spray and freeze-drying methods on aroma compounds, sensory characteristics, physicochemical composition, antioxidant and antimicrobial properties of instant sage (Salvia rosifolia Sm.) tea
Baltaci, Cemalettin; Akşit, Zeynep; Şidim, Muhammed
Sage is used as an aroma and spice in foods around the world. With its strong and even bitter taste, sage has been traditionally used as an effective solution to many health problems for centuries. It is a rich plant with many bioactive compounds. In this study, instant tea production was performed from the dried samples of the sage plant (Salvia rosifolia Sm.) using two drying methods; freeze-drying and spray-drying. The sensorial, physicochemical, antioxidant and antimicrobial properties of the products obtained by both methods were analyzed. Color values, solubility in water, moisture analysis (21.3% spray-dried; 4.0% freeze-dried), free radical scavenging activity (DPPH), Trolox Equivalent Antioxidant Capacity (TEAC), Ferric reducing/antioxidant power assay (FRAP), aroma analysis, protein analysis (5.5% spray-dried; 5.7% freeze-dried), ash, mineral, antimicrobial and sensory analyses were performed. Significant differences were found between two soluble tea obtained by the spray-drying and the freeze-drying methods. In general, the antioxidant capacities are higher in freze-dried samples. All of the 61 aroma components were detected in freeze dried samples while only 18 of them were detected in spray dried samples. In addition to their differencies; plenty of bioactive components, easy to use, ready to drink herbal tea have been produced with both techniques.
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