Sürdürülebilir Kalkınma Amaçları
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- ArticleAçık Erişim2023Thermosensitive In situ Gelling System for Dermal Drug Delivery of Rutin
Gözcü, Sefa; Polat, Kerem Heybet
Objectives: Rutin has been broadly applied for treating several diseases due to its pharmacological activities. However, its low aqueous solubility limits its absorption and bioavailability. This research aims to increase the solubility of rutin using cyclodextrin and to develop a temperature-triggered in situ gelling system for dermal application. Materials and Methods: The solubility of rutin was increased with sulfobutyl ether-β-cyclodextrin (SBE-β-CD). Rutin-SBE-β-CD inclusion complex was prepared by kneading and freeze dying method. Structural characterization was carried out using differential scanning calorimetry and fourier transform infrared spectroscopy. In situ gel formulations were prepared with pluronic F127 (PF127), a thermosensitive polymer, and chitosan (CH), a natural, biodegradable, and mucoadhesive hydrophilic polymer. In situ gel characteristics such as pH, clarity, gelation temperature, and viscosity were determined. Results: When the solubility diagrams were examined, it was concluded that SBE-β-CD showed a linear increase, therefore, AL-type diagram was selected. The formulations were produced using different amounts of PF127 and a fixed ratio of CH. Three in situ gels were evaluated for their pH, gelling temperature, and the rheological behaviors, and one formulation was selected. It was observed that the formulations had a pH between 6-6.1, and their gelation temperature decreased with increasing PF127 that was between 20°C to 34°C. For the selected formulation (formulation E3), 0.5% rutin and rutin/SBE-β-CD were transferred to the in situ gelling system. Because of in vitro release studies, it was observed that the release of the rutin/SBE-β-CD inclusion complex containing NZ formulation showed a higher burst effect than the others and the release continued for 6 hours. Conclusion: The results indicated that the combination of PF127 and CH can be a hopeful in situ gelling vehicle for dermal delivery of rutin and rutin/SBE-β-CD.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Isolation and cytotoxic activities of undescribed iridoid and xanthone glycosides from Centaurium erythraea Rafn. (Gentianaceae)
Akşit, Hüseyin; Gözcü, Sefa; Altay, Ahmet
Centaurium erythraea Rafn. (Gentianaceae) is used in internal traditional therapy as an anthelmintic, hypotensive, antipyretic, and antidiabetic. It is used externally for the treatment of wounds. Ursolic acid, maslinic acid, secologanin, secologanin dimethyl acetal, centauroside A, erythraeaxanthone I, erythraeaxanthone II, and demethyleustomin were isolated from aerial parts of Centaurium erythraea and were identified using spectroscopic methods, including NMR and mass spectrometry. The cytotoxic potency of undescribed compounds was evaluated by the XTT assay against human breast cancer MCF-7, MDA-MB-453 and mouse fibroblast 3T3-L1 cell lines. Erythraeaxanthone II was found to have the most potent cytotoxic activity.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Formulation Development of Dual Drug-Loaded Thermosensitive Ocular In Situ Gel Using Factorial Design
Polat, Heybet Kerem; Arslan, Aslıhan; Ünal, Sedat; Haydar, Muhammet Kerim; Aytekin, Eren; Gözcü, Sefa; Karakuyu, Nasıf Fatih; Mokhtare, Behzad
Purpose: To overcome the problems of low bioavailability of the drug associated with the short pre-corneal residence time, a thermoresponsive in situ gel system containing poloxamer P407, hydroxypropyl methylcellulose, and chitosan was developed to prolong the pre-corneal residence time of the drug. Methods: The central composite design was utilized to assess the effects of the concentration of poloxamer 407 hydroxypropyl methylcellulose and chitosan, the concentration of polymer, and the polymer type on the viscosity, pH, and gelation temperature, which were considered indicators of optimum formulations. Results: After model selection for response analysis, the quadratic model was found to be the best-fitting model for the relationship between independent factors and response variables. As a result of the central composite design, the optimized formulation contained 15.17% poloxamer 407 and 2.141% chitosan. Viscosity 25 °C = 2199.4 ± 26.2, viscosity 35 °C = 15,487.2 ± 117.4, pH = 6.5 ± 0.01, and gelation temperature °C = 33.3 ± 0.47 were obtained. The ex-vivo study revealed that the BRN formulation containing flurbiprofen-cyclodextrin inclusion complex has higher corneal penetration (P < 0.01). The cytotoxicity of ARPE-19 cells and irritation studies, as measured by in situ gel, was found to be acceptable. In lipoxygenase studies, the effectiveness of the BRN formulation was found to be significantly higher than other formulations (P < 0.01). Conclusions: It is thought that the BRN formulation may be an alternative to the treatment of ocular allergic disease.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2023Antidiabetic and antioxidant properties of Paeonia mascula L.: In vitro and in vivo studies, and phytochemical analysis
Gözcü, Sefa; Ugan, Rustem Anil; Özbek, Hilal; Gündoğdu, Betül; Guvenalp, Zuhal
The medicinal plant Paeonia mascula L. is commonly used in Anatolian folk medicine for its antidiabetic properties. This study aimed to investigate the in vitro α-glucosidase enzyme inhibition effect, in vivo antidiabetic, and antioxidant activities of extracts obtained from P. mascula. The in vivo studies were conducted on diabetic rats induced with streptozotocin. The ethyl acetate and n-butanol extracts showed the highest efficacy in both in vitro and in vivo experiments, reducing AST, ALT, and MDA levels while increasing GSH and SOD activities in rats. In total, seven compounds were isolated from both extracts, and their structures were identified using spectroscopic methods such as 1D and 2D NMR and Mass Spectrometry. The in vitro α-glucosidase inhibition assay on purified compounds revealed that 1,2,3,4,6-penta-O-galloyl-β-D-glucose was the most effective compound. These findings support the traditional use of P. mascula as an antidiabetic agent.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Design of Thymol Loaded Dental In Situ Gels; In Vitro Evaluation
Gözcü, Sefa; Akşit, Hüseyin; Polat, Heybet Kerem
Objetive: The aim of the study was to develop thymol thermosensitive in situ gels based on poloxamers in order to prolong dental contact time, control drug release, and enhance dental bioavailability. Materials and Methods: Poloxamer 188 (P188) and poloxamer 407 (P407) were used in varying amounts to designed the in situ gels. Mucoadhesive excipient, like hydroxypropyl methylcellulose (HPMC), was transferred to the in situ gels to improve the formulation's ability to adhere to biological surfaces. For the created formulations, in vitro drug release, pH, clarity, and sol-gel transition temperature were all evaluated.Results: The all of in situ gels gelation temperatures of the developed formulations range from 33 to 37°C, pH values are around 7, and syringeability is defined as the amount of force necessary to discharge each formulation from a syringe fitted with a 20-gauge needle. The quantities of P407 and HPMC with the preparations, decreasing in vitro burst release while also increasing the viscosity but every in situ gel formulation releases for six hours. Conclusion:The results show that in situ gels containing P407 and P188 show promise for thymol dental application.
RDL Kalıcı Kaydıİncele - Proje2022Deneysel Diyabet Oluşturulmuş Sıçanlarda Paeonia Mascula L. Ve Polygonum Cognatum Meissn. Bitki Ekstrelerinin Hipoglisemik Ve Antioksidan Etkilerinin Değerlendirilmesi, Etkiden Sorumlu Maddelerin Izolasyonu Ve Yapı Tayini.
Gündoğdu, Betül; Gözcü, Sefa; Ugan, Rüstem Anıl; Özbek, Hilal; Güvenalp, Zühal
Amaç: Polygonum cognatum Meissn. ve Paeonia mascula L. türlerinin herbalarından hazırlanan ana ekstre ve farklı polariteye sahip alt fraksiyonların in vitro antidiyabetik, in vivo diyabetik sıçanlar üzerinde tedavi edici ve koruyucu etkilerinin belirlenmesi, etkili ekstreler üzerinde saflaştırma çalışmaları, saflaştırılan bileşiklerin in vitro antidiyabetik etkinliği ve etkiden sorumlu bileşiklerin etkili ekstredeki miktarlarının HPLC ile tespit edilmesi çalışmamızın amacını oluşturmaktadır. Materyal ve Metot: Ekstrelerin ve saflaştırılan bileşiklerin in vitro antidiyabetik etkilerinin test edilmesi amacıyla ?-glukozidaz enzim inhibitör etki deneyi yapılmıştır. Ekstrelerin in vivo diyabet üzerine tedavi edici ve koruyucu etkilerinin belirlenmesi amacıyla Streptozotosin (STZ) ile deneysel diyabet oluşturulan 250-300 gr aralığında değişen Wistar albino türü sıçanlar kullanılmıştır. Bitki ekstreleri, günde 2 kere aynı dozda 10 gün boyunca uygulanmış ve 10 gün sonunda sıçanlar sakrifiye edilerek kan, pankreas, böbrek, kalp, göz ve karaciğer dokuları alınmıştır. Saflaştırılan bileşiklerin yapıları 1D-, 2D-NMR, UV ve Kütle spektroskopisi yöntemleri kullanılarak aydınlatılmıştır. Bulgular: Polygonum cognatum ve Paeonia mascula türlerinin herbalarından hazırlanan etil asetat ve n-butanol ekstreleri en yüksek in vitro ?-glukozidaz inhibitör etki göstermiştir. Yapılan in vivo çalışmaların sonuçlarına göre; aynı ekstreler deneysel diyabet oluşturulmuş sıçanların açlık kan şekerini yüksek oranda düşürmüştür. Buna ek olarak pozitif kontrol grubuna kıyasla, etil asetat ve n-butanol ekstrelerinin verildiği sıçanlarda AST, ALT ve MDA düzeyinde azalma, GSH düzeyinde ve SOD aktivitesinde artış olduğu tespit edilmiştir. İzolasyon çalışmalarında, P. cognatum?dan 11 bileşik, P. mascula?dan 8 bileşik olmak üzerek toplam 19 bileşik saflaştırılmıştır. Bu bileşikler üzerinde yapılan ?-glukozidaz inhibitör etki çalışması sonucunda P. cognatum ve P. mascula türlerinde etkiden sorumlu bileşikler sırasıyla kersetin (PCB-1) ve 1,2,3,4,6-penta-O-galloil-ß-D-glukoz (PME-1) olarak bulunmuştur. Sonuç: In vitro ?-glukozidaz enzim inhbisiyonu, in vivo deneysel diyabet oluşturulmuş sıçanlardan elde edilen biyokimyasal ve histopatolojik bulgular doğrultusunda halk arasında antidiyabetik amaçla kullanılan Polygonum cognatum ve Paeonia mascula türlerinin kan şekerini düşürdüğü, karaciğer enzimleri üzerine olumlu etkilerinin olduğu, antioksidan enzim düzeylerini önemli oranda arttırdığı, oluşan oksidatif stresi önlediği görülmüş ve etkiden sorumlu bileşikler tespit edilmiştir. Bu iki türün diyabette ve diyabetin neden olduğu komplikasyonlarda kullanılabileceği düşünülmüştür.
RDL Kalıcı Kaydıİncele - Review ArticleAçık Erişim2022Oküler İlaç Taşıyıcı Sistemler: Geleneksel Derleme
Unal, Sedat; Gözcü, Sefa; Polat, Heybet Kerem; Aytekin, Eren
Oküler ilaç uygulamalar ı, gözün yap ısal karmaşıklığı ve sa- vunma mekanizmaları nedeniyle oftalmologlar ve oküler ilaç ta şıyıcı sistemler üzerine çalışan bilim insanları için çok zorlu bir alandır. Farklı kornea katmanları, sklera, konjonktival kan akışı, gözyaşı seyreltmesi ve kan retina bariyeri gibi engeller, gözün anterior ve posterior kısım- larına ilaç uygulanması etkinliğini sınırlamaktadır. Konvansiyonel okü- ler ilaç uygulama şekilleri aras ında oküler göz damlas ı tüketiciler tarafından yaygın olarak tercih edilmektedir. Fakat bu göz damlalarının oküler bariyerlerden emilimi sınırlı olduğu için düşük biyoyararlanıma sahiptir bu sebeple sık uygulama gerektirmektedir. Bu durum ise hasta uyuncunu azaltmaktadır. Ayrıca bazı durumlarda etkin maddenin çö- zelti şeklinde hazırlanması mümkün olmamaktadır. Bu sebeple yine damla şeklinde kullanılabilen süspansiyon, emülsiyon gibi farklı dozaj şekilleri de geliştirilmeye çalışılmaktadır. Fakat bu tarz formülasyonlar da formülasyona bağlı stabilite ve yüksek viskozite gibi ciddi sıkıntılar barındırmaktadır. Hem gözün yap ısal sorunları hem de dozaj şekille- rinde istenilen etkilerin elde edilememesi ara ştırmacıları farklı çalış- malara itmektedir. Bu nedenle bilim insanlar ı, göze ilaç verme potansiyelini ve dolayısıyla tedavi etkinliğini artırmak için çeşitli ilaç taşıyıcı sistemleri tasarlamış ve incelemişlerdir. Nanopartikül, lipozom, mikroemülsiyon, insert ve ilaç yüklü kontakt lens gibi yeni ilaç taşıyıcı sistemler son zamanlarda geleneksel ilaç ta şıyıcı sistemlere alternatif olarak araştırılmaktadır. Bu tarz yeni ilaç taşıyıcı sistemler oküler ilaç uygulama açısından umut verici olsa da üstesinden gelinmesi gereken sorunlar da bulunmaktadır. Bu derlemede çeşitli geleneksel ve yeni ilaç taşıyıcı formülasyonlar özetlenmeye çalışılmıştır.
RDL Kalıcı Kaydıİncele - Book ChapterYalnız künye2022Aesculus hippocastanum L.
Gözcü, Sefa
Medicinal plants have been used in the treatment of various diseases since ancient times. Aesculus hippocastanum (horse chestnut) is a popular herb recognized worldwide and used in treatment. The seed extract of the plant contains phytocompounds such as flavonoids, hydroxycoumarins, proanthocyanidins, sterols, tannins, amino acids, and triterpenoid saponin glycosides. Studies have confirmed that Hippocastani semen extracts and pure substance have various activities, including anti-inflammatory, antibacterial, antifungal, antiviral, antiangiogenic (vascular protection), anti-obesity, antioxidative, antidiabetic, antidiabetic neuropathy, hepatoprotective, anticoagulant, antihematoma, antigenotoxic, anti-infertility, and cytotoxic properties. Due to the activity of A. hippocastanum extracts and their bioactive compounds, this herb may be useful as an alternative therapy for a variety of ailments.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2022Molecular Docking Study of Several Seconder Metabolites from Medicinal Plants as Potential Inhibitors of COVID-19 Main Protease
Bilginer, Sinan; Gözcü, Sefa; Güvenalp, Zuhal
Objectives: Coronaviruses (CoVs) cause infections that affect the respiratory tract, liver, central nervous, and the digestive systems in humans and animals. This study focused on the main protease (Mpro) in CoVs (PDB ID: 6LU7) that is used as a potential drug target to combat 2019-CoV. In this study, a total of 35 secondary metabolites from medical plants was selected and docked into the active site of 6LU7 by molecular docking studies to find a potential inhibitory compound that may be used to inhibit Coronavirus Disease-2019 (COVID-19) infection pathway. Materials and Methods: The chemical structures of the ligands were obtained from the Drug Bank (https://www.drugbank.ca/). AutoDockTools (ADT ver. 1.5.6) was used for molecular docking studies. The docking results were evaluated using BIOVIA Discovery Studio Visualizer and PyMOL (ver. 2.3.3, Schrodinger, LLC). Results: Pycnamine, tetrahydrocannabinol, oleuropein, quercetin, primulic acid, kaempferol, dicannabidiol, lobelin, colchicine, piperidine, medicagenic acid, and narcotine is found to be potential inhibitors of the COVID-19 Mpro. Among these compounds, pycnamine, which was evaluated against COVID-19 for the first time, showed a high affinity to the COVID-19 Mpro compared with other seconder metabolites and reference drugs. Conclusion: Our results obtained from docking studies suggest that pycnamine should be examined in vitro to combat 2019-CoV. Moreover, pycnamine might be a promising lead compound for anti-CoV drugs.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2019Phenolic compounds from Cotinus coggygria Scop. With alpha glucosidase inhibition
Özbek, Hilal; Yuca, Hafize; Gözcü, Sefa; Dursunoğlu, Benan; Özenver, Nadire; Güvenalp, Zühal; Kazaz, Cavit; Önal, Mehmet; Demi̇Rezer, L. Ömür
Diabetes mellitus (DM), characterized by hyperglycemia, is one of the serious metabolic disorders. Inhibiting the key enzymes in digestion of dietary starch such as α-amylase and α-glucosidase, is one of the methods in treatment of DM. Antidiabetic drugs that inhibit carbohydrate-hydrolyzing enzymes have undesired side effects therefore, there is a huge interest in search of medicinal plants. Cotinus coggygria Scop. (Anacardiaceae) is used in Turkish folk medicine to treat DM. Therefore the bioguided fractionation and isolation studies were carried on C. coggygria. The ethyl acetate fraction possessed significative α-glucosidase inhibitory effect with 8.2 µg/mL IC50 value but no meaningful α-amylase inhibitory effect. Gallocatechin (1), methyl gallate (2), myricetin-3-O-α-rhamnoside (3), myricetin-3-O-β-galactoside (4) and 1, 2, 3, 4, 6-penta-O-galloyl-β-glucose (5) were isolated from this active fraction. Compound 5 showed significant α-glucosidase inhibitory activity with 1.5 µg/mL IC50 value, when compared to acarbose (IC50 = 3364.2 µg/mL) which used as positive control.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2019Qualitative and quantitative determination of the effective components of the plants in different herbal slimming products in Turkey by HPLC
Dursunoğlu, Benan; Yuca, Hafize; Gözcü, Sefa; Yilmaz, Bilal; Güvenalp, Zühal
Obesity is a serious disease that can be due to genetic and environmental reasons and is defined by the World Health Organization as abnormal or excessive fat accumulation that may impair health. There is an increasing trend towards herbal slimming products in obesity treatment. The quality control analysis of these products is important for public health. In this study, new, simple, and sensitive high performance liquid chromatography methods were developed for qualitative and quantitative determination of arbutin, hydroquinone, ursolic acid, chlorogenic acid, epigallocatechin gallate, epigallocatechin, epicatechin gallate, and catechin in eleven herbal slimming products in Turkey containing Camellia sinensis, Ilex paraguariensis, and Calluna vulgaris. The intra- and interday precisions, stated as the relative standard deviation, were less than 2% and accuracy, based on relative error, was less than 10%. The limits of detection and quantification for arbutin and hydroquinone were 0.80 and 2.40 µg/mL and for the other compounds were 0.30 and 0.90 µg/mL, respectively. The methods developed can be used for routine quality control analysis of these effective compounds in different herbal slimming products.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018α-amylase and α-glucosidase inhibitory activities of the extracts and constituents of ferulago blancheana, F. Pachyloba and F. Trachycarpa roots
Karakaya, Songül; Özbek, Hilal; Gözcü, Sefa; Güvenalp, Zühal; Yuca, Hafize; Duman, Hayri; Kazaz, Cavit; Kiliç, Ceyda Sibel
Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC50 = 4.9 mg/mL). Grandivitinol (IC50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018Simultaneous determination of arbutin and hydroquinone in different herbal slimming products using gas chromatography-mass spectrometry; [Arbutin ve hidrokinonun farklı bitkisel zayıflama ürünlerinde gaz kromatografisi-kütle spektrometresi ile eşzamanlı belirlenmesi]
Dursunoğlu, Benan; Yuca, Hafize; Güvenalp, Zühal; Gözcü, Sefa; Yilmaz, Bilal
Objectives: The aim of our study was to develop a simple, precise, sensitive and specific method for the simultaneous determination of arbutin and hydroquinone in different herbal slimming products using GC-MS. Materials and Methods: The methanol and aqueous extracts of nine herbal slimming products in Turkey were evaluated for analysis of arbutin and hydroquinone using GC-MS method. Results: The retention times of arbutin and hydroquinone were found as 11.32 and 5.44 min, respectively. The linear ranges in this method were 5-500 ng/mL for arbutin and hydroquinone, respectively. The intra-and inter-day precisions, expressed as the relative standard deviation, were less than 1.94 and 2.73%, determined from quality control samples for arbutin and hydroquinone, and accuracy was within 1.13 and 2.56% in terms of relative error, respectively. The limit of detection and quantification were 0.555 and 1.665 ng/mL for arbutin, and 0.031 and 0.093 ng/mL for hydroquinone, respectively. Conclusion: The developed method can be used for routine quality control analysis of arbutin and hydroquinone in different herbal slimming products.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2018The α-amylase and α-glucosidase inhibitory activities of the dichloromethane extracts and constituents of Ferulago bracteata roots
Karakaya, Songül; Gözcü, Sefa; Güvenalp, Zühal; Özbek, Hilal; Yuca, Hafize; Dursunoğlu, Benan; Kazaz, Cavit; Kılıç, Ceyda Sibel
Context: Ferulago (Apiaceae) species have been used since ancient times for the treatment of intestinal worms, hemorrhoids, and as a tonic, digestive, aphrodisiac, or sedative, as well as in salads or as a spice due to their special odors. Objectives: This study reports the α-amylase and α-glucosidase inhibitory activities of dichloromethane extract and bioactive compounds isolated from Ferulago bracteata Boiss. & Hausskn. roots. Materials and methods: The isolated compounds obtained from dichloromethane extract of Ferulago bracteata roots through bioassay-guided fractionation and isolation process were evaluated for their in vitro α-amylase and α-glucosidase inhibitory activities at 5000-400 µg/mL concentrations. Compound structures were elucidated by detailed analyses (NMR and MS). Results: A new coumarin, peucedanol-2′-benzoate (1), along with nine known ones, osthole (2), imperatorin (3), bergapten (4), prantschimgin (5), grandivitinol (6), suberosin (7), xanthotoxin (8), felamidin (9), umbelliferone (10), and a sterol mixture consisted of stigmasterol (11), β-sitosterol (12) was isolated from the roots of F. bracteata. Felamidin and suberosin showed significant α-glucosidase inhibitory activity (IC50 0.42 and 0.89 mg/mL, respectively) when compared to the reference standard acarbose (IC50 4.95 mg/mL). However, none of the tested extracts were found to be active on α-amylase inhibition. Discussion and conclusions: The present study demonstrated that among the compounds isolated from CH2Cl2 fraction of F. bracteata roots, coumarins were determined as the main chemical constituents of this fraction. This is the first report on isolation and characterization of the bioactive compounds from root extracts of F. bracteata and on their α-amylase and α-glucosidase inhibitory activities.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2017α-Amylase and α-glucosidase inhibitory activities of the herbs of Artemisia dracunculus L. and its active constituents
Güvenalp, Zühal; Özbek, Hilal; Dursunoğlu, Benan; Yuca, Hafize; Gözcü, Sefa; Çil, Yeşim M.; Kazaz, Cavit; Kara, Kemalettin; Demirezer, Ömür L.
A new phenylpropanoid named 4-(1′,1′,2′,2′-tetramethylpropyl)-1,2-benzenediol (1) with a known phenylpropanoid: 3-(p-methoxyphenyl)-1,2-propanediol (2), a coumarin: herniarin (3), and a flavonol glycoside: rutin (4) were isolated from the aerial parts of Artemisia dracunculus L. Their structures were elucidated by detailed analyses of 1D and 2D nuclear magnetic resonance, IR, ESI–MS and HR–ESI–MS data. Methanol extract of aerial parts of the plant, different polarity fractions of methanol extract and the isolated compounds from these fractions were evaluated for their alpha amylase and alpha glucosidase inhibitory effects. Phenylpropanoids containing fraction had a significantly higher α-glucosidase inhibitory activity (90.4%) when compared with the standart compound acarbose (26.0%) at 1 mg/mL. However none of the tested extracts or compounds were found to be active on α-amylase inhibition.
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