Sürdürülebilir Kalkınma Amaçları
Yayınlar
- ArticleAçık Erişim2027From extraction to ocular delivery: An integrated approach using apigenin and apigenin 7-O-glucoside nanosuspensions to treat corneal neovascularization
Aytekin, Eren; Gözcü, Sefa; Polat, Heybet Kerem; Akşit, Hüseyin; Karakuyu, Nasıf Fatih; Kurt, Nihat; Sofu, Gülşah Usta; Akkaya, Züleyha Yalnız; Tebrizi, Behzat; Çalış, Sema
Corneal neovascularization (CV) is a vision-threatening condition characterized by the pathological ingrowth of blood vessels. While corticosteroids and anti-VEGF agents are standard treatments, their prolonged use is associated with significant ocular side effects. This study aimed to develop and optimize nanosuspensions of Apigenin (APG) and Apigenin-7-O-glucoside (APG 7) to enhance their ocular bioavailability and evaluate their efficacy in the treatment of CV. APG and APG 7 were isolated from Petroselinum crispum . Nanosuspensions were formulated using an anti-solvent precipitation method, optimized via a 23 factorial design considering PVA concentration, Span 80 concentration, and homogenization speed. The physical properties, including particle size, PDI, and zeta potential, were characterized. In vivo efficacy was assessed over a 7-day treatment period in an alkali-burn induced rat CV model through clinical scoring, histopathology, and immunohistochemical analysis of VEGFA and IL-6 expression. The optimized APG nanosuspensions exhibited a mean particle size of ∼166.9 nm with high stability. In vivo studies demonstrated that 0.05% APG nanosuspension significantly inhibited corneal angiogenesis, reduced stromal edema, and downregulated VEGFA and IL-6 expression compared to the untreated injury group (p < 0.05). Conversely, APG 7 formulations failed to show significant therapeutic efficacy, likely due to limited corneal permeability attributed to the glycoside moiety. The 0.05% APG nanosuspension presents a promising, non-invasive therapeutic alternative for CV management, offering effective anti-angiogenic activity with improved ocular delivery characteristics.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Chemical Composition and Antibacterial Activity of the Essential Oil from Cupressus arizonica Greene Cones Against Clinical and Foodborne
Gözcü, Sefa; Akşit, Zeynep; Dursun, Ahmet
Objective: The purpose of this study was to characterize the chemical composition of the essential oil (EO) derived from the cones of Cupressus arizonica Greene collected from Erzincan and to evaluate its antibacterial potential against a range of pathogenic microorganisms, including strains not previously investigated for this species. Methods: The essential oil was obtained from fresh cones via hydrodistillation for 4 hours. Chemical profiling was performed using Gas Chromatography-Mass Spectrometry (GC-MS). The antibacterial activity was assessed against six pathogenic bacteria using the disc diffusion method, with imipenem serving as a positive control. Results: GC-MS analysis identified 35 compounds, representing 93.83% of the total oil. The major components were α -pinene (53.02%), 3-carene (13.31%), α -myrcene (4.54%), sabinene (4.24%), and limonene (3.47%). The oil exhibited selective antibacterial activity, with inhibition zones recorded for Salmonella enterica (10.0 ± 0.3 mm), Clostridium perfringens (8.1 ± 0.5 mm), and Bacillus cereus (8.0 ± 0.6 mm). However, no inhibitory effect was observed against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. Conclusion: This study identifies a distinct α-pinene/3-carene chemotype of C. arizonica influenced by the regional flora of Erzincan. Notably, this research represents the first report of the inhibitory efficacy of C. arizonica cone essential oil against the anaerobic pathogen Clostridium perfringens. The findings suggest that this essential oil could be a promising natural candidate for targeted applications against certain foodborne and anaerobic pathogens.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Development and characterization of olopatadine and verbascoside-loaded thermoresponsive in situ gels for ocular drug delivery: a factorial design approach for allergic conjunctivitis
Polat, Heybet Kerem; Çopur, Tuğba; Pezik, Esra; Turanlı, Yasin; Karakuyu, Nasıf Fatih; Köngül, Esra; Ünal, Sedat; Gözcü, Sefa; Sarman, Emine
Objective: Allergic conjunctivitis is one of the most prevalent conditions affecting the eyes, and various medications are used in its treatment. In this study, olopatadine and verbascoside were used in combination. Methods: A factorial design was employed to evaluate the effects of Poloxamer 407 and sodium alginate. The impact of polymer concentration on parameters such as viscosity, gelation time, mucoadhesion, pH, and gelation temperature–considered indicators of ideal formulations–was assessed. Results: The optimal formulation, determined through the factorial design, included 0.5% sodium alginate and 20% poloxamer 407. The obtained values were viscosity at 25 °C = 7451.8 cP, viscosity at 35 °C = 11367.3 cP, pH = 7.12, gelation temperature = 30 °C, gelation time = 52 s, and mucoadhesion = 0.301 N. Cytotoxicity tests on ARPE-19 and L929 cells, along with irritation studies, indicated acceptable safety profiles. In vitro release tests demonstrated an initial burst release of verbascoside and olopatadine during the first 2 h, followed by a controlled release over the subsequent 8 h. Mathematical analysis of the release kinetics revealed a good fit with the Weibull model. No adverse effects were observed in rats during Draize’s in vivo test. Conclusions: Consequently, the drug-loaded in situ gel formulation may be a promising option for treating allergic conjunctivitis.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2026Chemical Composition and Antibacterial Activity of Rosa villosa subsp. mollis R. Keller & Gams., Rosa hemisphaerica J. Herrm. and Natural Hybrid Rosa ozcelikii Korkmaz & Kandemir Essential Oil
Gözcü, Sefa; Akşit, Zeynep; Akşit, Hüseyin; Şimşek, Samed; Korkmaz, Mustafa
This study explores the essential oils (EOs) of Rosa hemisphaerica (RH), Rosa villosa subsp. mollis (RV) and the newly identified hybrid Rosa ozcelikii (RO). The major components of the EOs were found to be nonadecane (22.45%), viridiflorol (19.37%) and heneicosane (11.72%) in RH; α-muurolene (14.72%), σ-cadinene (17.88%) and farnesol (9.94%) in RV; and heptacosane (27.63%), heneicosane (12.42%) and α-citronellol (11.90%) in RO. Antibacterial activities were evaluated using the disc diffusion method. The EOs demonstrated moderate antibacterial effects: RV and RH exhibited inhibition zones of 8.0 ± 0.4 mm against Pseudomonas aeruginosa, while RO showed stronger activity with inhibition zones of 11.0 ± 0.5 mm against Staphylococcus aureus, 12.0 ± 0.4 mm against Clostridium perfringens and 9.0 ± 0.5 mm against Bacillus cereus. These findings highlight the chemical diversity and potential therapeutic applications of these Rosa species, emphasising their relevance in the search for natural antimicrobial agents.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2026Therapeutic Effects of Cichorium intybus L. Extract in an Experimentally Induced Insulin Resistance Model in Rats
Ermis, Uğur; Gül, Hüseyin Fatih; Gözcü, Sefa; Özdemir, Bengül; Hamdemir, Özgecan; Binnetoğlu, Damla
Introduction: Insulin resistance (IR) is a central metabolic feature of polycystic ovary syndrome (PCOS). Althou gh metformin improves insulin sensitivity, its adverse effects limit long-term use, prompting interest in herbal alternatives. The present study aimed to investigate the effects of Cichorium intybus L. ethanolic extract on PCOS-associated IR in a letrozole-induced rat model, compared with metformin. Materials and Methods: A total of twenty-four female Sprague–Dawley rats were allocated into four groups: healthy control (H), PCOS-associated insulin resistance (IR), metformin-treated (MET), and Cichorium intybus ethanolic extract–treated (C.I). PCOS was induced by oral letrozole administration (1 mg/kg/day) for 21 days. Following confirmation of IR by estrous cycle disruption and oral gl ucose tolerance test (OGTT), treatments were administered orally for 15 da ys. Metabolic parameters, serum insulin, glucose, lipid profile, sex hormones, and HOMA-IR index were evaluated. Skeletal muscle tissues were examined histopathologically and by immunohistochemical analysis of insulin receptor (INSR), insulin receptor subsrate 1 (IRS1), and insulin receptor subsrate 2 (IRS2) expression. Results: PCOS-associated IR rats showed impaired glucose tolerance, hyperinsulinemia, increased HOMA-IR, dyslipidemia, and hormonal imbalance. C. intybus ethanolic extract treatment significantly improved glucose tolerance, insulin levels, HOMA-IR, and lipid profile, with effects comparable to metformin. While metformin markedly restored INSR, IRS1, and IRS2 expression in skeletal muscle, C. intybus produced only moderate improvements. Conclusion: C. intybus ethanolic extract improved metabolic, hormonal, and biochemical paramete rs in PCOS-associated IR. However, its effects on skeletal muscle insulin signaling markers were less pronounced than metformin, suggesting a complementary therapeu tic role mainly through systemic metabolic regulation.
RDL Kalıcı Kaydıİncele - Review ArticleAçık Erişim2026From nature to nanoscale: advances, challenges, and preclinical translation of nanofiber drug delivery systems containing plant-derived active ingredients
Polat, Heybet Kerem; Turanlı, Yasin; Kurt, Nihat; Gözcü, Sefa; Tunçel, Emre; Bacanak, Hilal; Aytekin, Eren; Ünal, Sedat
Since the early 1960s, nanotechnology has been a critical area of science, allowing for the development of sophisticated nanomaterials. Nanofibers, one of the most widely used nanotechnological drug delivery systems, have emerged as a highly versatile platform within modern pharmaceutical sciences. By combining various polymers with active herbal ingredients, these systems mimic the natural extracellular matrix and provide improved functions such as a high surface area-to-volume ratio, drug targeting, and controlled drug release. Preclinical studies demonstrate that phytochemical-containing nanofibers have improved therapeutic profiles, including higher anti-inflammatory, antioxidant, antimicrobial, and antineoplastic effects. In various biomedical applications, such as promoting tissue engineering (such as bone and nerve regeneration), limiting tumor growth, and accelerating wound closure, it was shown that nanofibers may overcome the physicochemical limitations of herbal drugs such as low solubility and bioavailability. Despite promising preclinical study results, there are significant obstacles in the way of the commercialization of herbal drug-loaded nanofibers. The difficult standardization of multi-component herbal extracts, stabilization drawbacks, and the absence of scalable industrial manufacturing equipment that can maintain repeatable characterization of nanofibers are some of the major obstacles, in addition to a lack of clinical trials. To navigate the clinical translation of nanofibers, interdisciplinary collaboration regarding quality control, safety, and regulatory pathways is strictly necessary due to the case-by-case review approach utilized by regulatory bodies such as the US Food and Drug Administration (FDA) and European Medicines Agency (EMA). This review offers a comprehensive overview of research on herbal drug-loaded nanofibers and contributes a novel perspective with regulatory and clinical translational insights.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Antioxidant Potential and Phenolic Content of Taraxacum officinale Root Extract
Gözcü, Sefa; Yılmaz, Aslı
Objective: The purpose of this study was to assess the antioxidant capability and bioactive chemical profile of Taraxacum officinale roots, a medicinal plant with potential therapeutic uses, collected from Erzincan, Türkiye. Methods: 70% methanol was used to extract the roots, and the solvent was then evaporated. Both the total flavonoid quantities (TFQ) and the total phenolic quantities (TPQ) were measured. The DPPH, ABTS, and FRAP tests were used to measure antioxidant capability. Results: The IC50 values for DPPH and ABTS assays were 50.47 ± 0.41 μg/mL and 70.57 ± 0.44 μg/mL, respectively. The reducing power, measured by the FRAP assay, was 26.87 ± 0.12 mg Trolox equivalents/g extract. The TPQ was 23.58 ± 0.97 mg gallic acid equivalents/g extract, and the TFQ was 7.76 ± 0.81 mg quercetin equivalents/g extract. Conclusion: The root extract of T. officinale collected from Erzincan exhibited low antioxidant activity despite its high phenolic and flavonoid content. This finding suggests that its potential as a natural antioxidant source in the food and pharmaceutical industries may be limited. Notably, this study represents the first investigation of T. officinale from Erzincan, Türkiye, highlighting the unique chemical composition of the region's flora.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Plantago major'un Antioksidan Potansiyeli ve Fenolik İçeriği
Gözcü, Sefa; Atmaca, Muhammed
Amaç: Bu çalışma, terapötik potansiyeli sahip, tıbbi bir bitki olan Plantago major'un toprak üstü kısımlarının antioksidan kapasitesini ve biyoaktif bileşik miktarını değerlendirmeyi amaçlamıştır. Yöntemler: Toprak üstü kısımlar %70 metanol kullanılarak ekstre edilmiş ve çözücü buharlaştırılmıştır. Toplam fenolik içerik (TPC) ve toplam flavonoit içerik (TFC) miktarları belirlenmiştir. Antioksidan kapasitesi DPPH, ABTS ve FRAP analizleri ile değerlendirilmiştir. Bulgular: DPPH serbest radikal süpürme testi IC50 değerini 127,33 ± 1,07 μg/mL olarak gösterirken, ABTS testi IC50 değerini 46,74 ± 2,53 μg/mL olarak ortaya koymuştur. FRAP analizi ise indirgeme gücünün 44,62 ± 0,61 mg TE/g ekstrakt eşdeğerinde olduğunu göstermiştir. Sonuç: Plantago major ekstraktının yüksek fenolik ve flavonoid içeriği, güçlü antioksidan aktivitesine önemli ölçüde katkıda bulunmaktadır. Bu bulgular, bitkinin doğal bir antioksidan kaynağı olarak farmasötik ve nutrasötik endüstrilerinde potansiyel kullanımını desteklemektedir.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2025Comprehensive phenolic profiling and biological evaluation of Centaurea glastifolia L. (Asteraceae)
Gözcü, Sefa; Akşit, Zeynep; Aydın, Ali; Yılmaz, Mustafa Abdullah; Şimşek, Samed
The present investigation focused on the comprehensive analysis of the phenolic profile of Centaurea glastifolia L. (Asteraceae) and the assessment of its diverse biological activities. Utilising LC-MS/MS, the phytochemical composition of the 70% methanol extract of Centaurea glastifolia (CG-ME) was thoroughly elucidated, revealing the presence of 30 distinct phytochemical compounds. Notably, major phenolic constituents identified in the extract included quinic acid, chlorogenic acid, luteolin-7-O-glucoside, kaempferol-3-O-glucoside, luteolin, and apigenin-7-O-glucoside. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities of CG-ME were investigated. The CG-ME exhibited a moderate capacity for scavenging DPPH radicals (IC50: 50.05 ± 1.58 µg/mL) and FRAP (63.96 ± 0.39 mg TE/g extract), indicating a moderate level of antioxidant activity. Moreover, CG-ME demonstrated significant antiproliferative effects (GI50: 1.10 and 1.30 µg/mL) on cancer cells (C6 and HTC cancer cell lines, respectively) while displaying low cytotoxicity towards normal cells (LC50: >1000 µg/mL). In terms of antibacterial activity, CG-ME was found to be inactive against tested both Gram-positive and Gram-negative bacterial strains (MIC > 500 µg/mL). The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5–14.2%) against normal cells.
RDL Kalıcı Kaydıİncele - Book ChapterYalnız künye2025Catnip (Nepeta cataria L. Lamiaceae)
Yigit, Belkis Muca; Cinbilgel, Ilker; Gözcü, Sefa; Tel, Ahmet Zafer; Ali, Ahmad
Nepeta cataria L. (Catnip) belongs to the genus Nepeta of the family Lamiaceae. The genus Nepeta is represented by 279 species, and it is widespread in the world. N. cataria was first described from Europe by Linne in 1753 and it originated from Europe and Asia. N. cataria, which is a perennial herb in terms of its systematic features, is a species with an erect stem, ovate leaves, large paniculate inflorescences, and glandular hairs. Its flowering time is between June and August. N. cataria, which is an economically cultivated herb, has been grown in many regions of Europe and Asia extensively. It needs alkaline and well-drained soils for agricultural activities. The plant is known as 'catnip' because cats like the odor of the plant. But different names such as 'catmint', 'catswort', 'cold grass' are also given. It also has traditional uses. Traditional medical benefits of catnip include stomachic illnesses, respiratory diseases, and stimulant effects. In history, there are also records of British executioners chewing the leaves of this plant during execution to benefit from its hallucinogenic effect. Fresh plants contain 0.2-0.7% essential oil. Catnip is a plant that produces a variety of biologically bioactive compounds, including terpenes, steroids, phenolic acids, phenylethanoid glycosides, and flavonoids. Of these chemical components, nepetalactone, actinidine, and beta phenylethyl alcohol provide the hallucinogenic effect of the plant. Catnip has anticonvulsant, myorelaxant, antidiabetic, anti-inflammatory, analgesic, anti-nociceptive, antimicrobial (antiviral, antibacterial, antifungal), antioxidant, anthelmintic, cytotoxic, anticancer and apoptotic, hepatoprotective, immunomodulatory, nematocidal, trypanocidal effects. In addition to these, catnip is also used as nematocidal, insecticidal, and insect repellent.
RDL Kalıcı Kaydıİncele - Review ArticleYalnız künye2025Herbal drug delivery for ocular treatments—an updated review
Polat, Heybet Kerem; Tunçel, Emre; Gözcü, Sefa; Turanlı, Yasin; Karakuyu, Nasıf Fatih; Ünal, Sedat
The aim of this review is to address the challenges faced by traditional dosage forms in ocular herbal drug delivery and to demonstrate the potential offered by nanotechnology to overcome related challenges. This study employed a systematic investigation approach, focusing on nanocarrier systems and advanced material-based strategies documented in the literature. Various nanoformulation systems, including in situ gels, liposomes, ethosomes, transfersomes, exosomes, polymeric and lipid-based nanoparticles, emulsions, nanofibers, and dendrimers, are extensively evaluated. The contribution of innovative nanomaterials, such as nanozymes and metallic and ceramic nanoparticles, to herbal drug delivery is assessed. Different herbal secondary metabolites with potential indications for ocular diseases are reviewed. The reported findings in the literature demonstrate that nanotechnology can enhance the solubility and stability of plant-derived active ingredients, enabling controlled release in target tissues and thus enhancing therapeutic efficacy. Furthermore, safety, toxicity, and regulatory frameworks are scrutinized. In summary, this review highlights the opportunities offered by nanotechnology-based systems in herbal medicine applications and guides integrating of alternative herbal therapies with allopathic approaches in the future.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2025Chemical Composition and Antimicrobial Activity of Hesperis isatidea (Boiss.) D.A.German & Al-Shehbaz Essential Oil
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Akşit, Hüseyin
The essential oils of aerial parts of Hesperis isatidea were analyzed using gas chromatography-mass spectrometry for the first time in the present study. The major components were determined as benzyl salicylate (13.40%), phytol (9.66%), benzyl benzoate (8.57%), undecanal (8.45%), and linalool (5.71%). The essential oil exhibited notable antibacterial activity against Clostridium perfringens, with a minimum inhibitory concentration (MIC) of 125 µg/mL, and Escherichia coli, with an MIC of 62.5 µg/mL. However, it showed limited effectiveness against Staphylococcus aureus and Listeria monocytogenes, both with an MIC of 250 µg/mL, and no antibacterial activity against Enterococcus faecalis and Bacillus cereus. The results demonstrate that the essential oil possesses selective antibacterial properties, especially against specific Gram-negative bacteria, indicating its potential as a valuable complementary antimicrobial agent.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Antimicrobial Activity and Chemical Composition of Essential Oil Extracted from Hyoscyamus niger L. Inflorescence
Gözcü, Sefa; Akşit, Hüseyin; Akşit, Zeynep
This study aimed to analyze the chemical composition and evaluate the antibacterial activity of essential oils extracted from the H. niger inflorescence collected from Erzincan, Türkiye. The essential oils were extracted using hydrodistillation and analyzed by GC-MS, identifying 23 components, with major constituents being 10-heneicosene (35.72%), phytol (20.50%), and acetic acid, butyl ester (10.10%). The antibacterial activity was tested against six bacterial strains, including Staphylococcus aureus, Clostridium perfringens, Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, and Escherichia coli, using the disc diffusion method. The H. niger inflorescence essential oil exhibited moderate antibacterial activity, specifically against Clostridium perfringens, with a zone of inhibition of 11.7 ± 1.2 mm compared to the positive control imipenem (17.0±1.0 mm). The results suggest that H. niger inflorescence essential oil has potential as an antibacterial agent, highlighting the importance of exploring plant-derived compounds as alternatives to traditional antibiotics in combating multidrug-resistant bacteria.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Phytochemical analysis and biological evaluation of Ferulago setifolia K. Koch
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydın, Ali; Yılmaz, Mustafa Abdullah; Akşit, Hüseyin
BACKGROUND: Ferulago setifolia K. Koch (Apiaceae) has been the subject of this study, aiming to comprehensively determine its phenolic fingerprint and evaluate its various biological activities. The liquid chromatography–tandem mass spectrometry analysis of the 70% methanol extract of F. setifolia (FS) revealed the presence of 23 phytochemicals, among which chlorogenic acid, quinic acid, kaempferol-3-O-glucoside, and quercetin-3-O-glucoside were identified as the major phenolics in the extract. RESULTS: The biological screening included examinations of antioxidant, antibacterial, antiproliferative, and cytotoxic activities. The FS extract displayed moderate 2,2-diphenyl-1-picrylhydrazyl radical scavenging and ferric-reducing capacity, indicating moderate antioxidant activity. Furthermore, FS exhibited significant antiproliferative effects on cancer cells while showing low cytotoxicity on normal cells. The antibacterial activity findings revealed that FS demonstrated potent activity against Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, and Escherichia coli. CONCLUSION: The findings of this study suggest that the methanolic extract of FS holds promise as a potential source of biologically active compounds. It can be utilized for the development of pharmaceutical formulations, thanks to its significant antiproliferative and antibacterial activities. Additionally, FS can serve as a valuable source of chlorogenic acid for industrial applications.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024TRADITIONAL USES OF MEDICINAL PLANTS IN ERZİNCAN PROVINCE, TÜRKİYE; [ERZİNCAN (TÜRKİYE) İLİNDEKİ TIBBİ BİTKİLERİN GELENEKSEL KULLANIMLARI]
Gözcü, Sefa; Korkmaz, Mustafa; Çorlu, Sercan; Tuysuz, Suat
Objective: This research was carried out to record the usage of plants, parts of this plant used, and methods of preparation by people living in 10 districts and 58 villages of Erzincan province. Material and Method: The medicinal plant species used by the local people for treatment, was collected and determined. All traditionally used information was collected; herbarium materials were prepared, They are deposited in Erzincan Binali Yıldırım University herbarium (EBYU), Erzincan Binali Yıldırım University. Result and Discussion: A total of 100 medical plants taxa pertaining to 39 families were defined in this research. Out of these, 88 species grew naturally, while 12 species were cultivated. The most widespread plant families were Asteraceae (14), Lamiaceae (8), and Rosaceae (14). Infusion was the most widely used preparation method. The utilization of traditional medicine was still extensive among the people in Erzincan. However, through increscent health service facilities in region, herbal medicine seemed to be more related to health care and illness prevention than curation. There is also the loss of traditional knowledge as it receives new immigrants. There is a gradual loss of traditional knowledge on the use of medicinal plants, both in younger generations and due to migration.
RDL Kalıcı Kaydıİncele - ArticleYalnız künye2024Gingerol containing polymeric nanofibers: a healing touch for accelerated wound recovery
Polat, Heybet Kerem; Gözcü, Sefa; Ünal, Sedat; Paçacı, Timur; Aytekin, Eren; Karakuyu, Nasıf Fatih; Köngül Şafak, Esra; Gültekin, Yakup; Yazıksız, Yonca; Kurt, Nihat
Objective: In the current research, 6-gingerol (GA)-loaded nanofiber drug delivery system were developed, and their potential usage in wound healing was evaluated. Significance: This study investigates the effectiveness of nanofibrous membranes composed of sodium alginate (SA), poly(vinyl alcohol) (PVA), and 6-gingerol (GA) as delivery systems for anti-inflammatory agents in the context of wound dressings. Methods: GA-loaded SA/PVA nanofiber was prepared using electrospinning. In vitro characterization of this nanofiber included the examination of comprehensive in vitro characterization, anti-inflammatory and antioxidant activities, cytotoxicity, a scratch tes and in vivo skin test. Results: GA was extracted from Zingiber officinale, and its successful isolation was confirmed through analyses such as H-NMR, C-NMR. Then GA was electrospuned into the SA/PVA nanofibers, and scanning electron microscopy (SEM) imaging revealed that the fiber diameters of the formulations ranged between 148 nm and 176 nm. Anti-inflammatory and antioxidant studies demonstrated that the effectiveness of GA increased with higher doses; however, this increase was accompanied by decreased cell viability. In vitro release studies revealed that GA exhibited a burst release within the first 8 h, followed by a controlled release, reaching completion within 24 h. Within the scope of in vitro release kinetics, release data are mathematically compatible with the Weibull model with high correlation. The scratch test results indicated that TB2 (%1 GA) promoted epithelialization. Furthermore, it was determined that TB2 (%1 GA) did not cause any irritation. Conclusions: As a result, TB2 shows promise as a formulation for wound dressings, offering potential benefits in the field of wound care.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024LC-MS /MS characterization and biological activities of Morina persica L. (Caprifoliaceae)
Gözcü, Sefa; Akşit, Zeynep; Şimşek, Samed; Kandemir, Ali; Aydin, Ali; Yilmaz, Mustafa Abdullah; Akşit, Hüseyin
The study aimed to comprehensively identify the phenolic fingerprint of Morina persica L. (Caprifoliaceae) and evaluate its various biological activities. LC-MS/MS analysis of 70% Morina persica methanol extract revealed the presence of 27 phytochemicals, with quinic acid, chlorogenic acid, and rutin being the major phenolics. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities were evaluated for biological screening. The results showed that the methanolic extract of M. persica has a moderate DPPH radical scavenging and ferric-reducing capacity, indicating antioxidant activity. M. persica was observed to have a sufficient antiproliferative effect against cancer cells and low cytotoxicity against normal cells. Moreover, M. persica demonstrated good antibacterial activity against Clostridium perfringens, Enterococcus faecalis, and Escherichia coli. These data suggest that the methanolic extract of M. persica could be considered both an industrial source of quinic acid and a potential biologically active ingredient for developing drug formulations.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Evaluation of antidiabetic and antioxidant effects of Polygonum cognatum Meisn. and phytochemical analysis of effective extracts
Gözcü, Sefa; Uğan, Rüstem Anıl; Özbek, Hilal; Gündoğdu, Betül; Güvenalp, Zühal
Polygonum cognatum Meisn. (Polygonaceae) is used both as food and as a folk medicine to treat diabetes. This study aimed to evaluate the effect of the extracts, along with isolated compounds, from P. cognatum aerial parts on diabetes. In vitro studies were conducted using an α-glucosidase inhibitory assay, while in vivo antidiabetic studies were carried out on streptozotocin-induced diabetic rats. Effective extracts were subjected to isolation studies, and structures of the compounds were elucidated by spectroscopic methods. The ethyl acetate and n-butanol extracts had the highest effect in both in vitro and in vivo experiments. They also decreased aspartate transaminase, alanine transaminase and malondialdehyde levels, while increasing glutathione and superoxide dismutase activity in rats. From the active extracts, 11 phenolic compounds were isolated and characterized. Among the isolated compounds, quercetin was found to be the most active according to α-glucosidase inhibitory activity studies. This study provided scientific evidence for the traditional use of P. cognatum as a folk medicine for treating diabetes. The findings suggest that the ethyl acetate and n-butanol extracts, as well as quercetin, have the potential for development as antidiabetic agents.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS IN AĞRI PROVINCE, TÜRKİYE: EXPLORING TRADITIONAL KNOWLEDGE AND THERAPEUTIC POTENTIAL; [TÜRKİYE'NİN AĞRI İLİNDE TIBBİ BİTKİLERİN ETNOBOTANİK ÇALIŞMASI: GELENEKSEL BİLGİ VE TEDAVİ POTANSİYELİNİN KEŞFEDİLMESİ]
Muca Yiğit, Belkıs; Gözcü, Sefa
Objective: This study was conducted to systematically document the use of plants, plant parts and preparation methods used by people in eight districts and sixty villages in Ağrı province. Material and Method: The medicinal plant species employed by the indigenous population for therapeutic purposes were systematically gathered and identified. Comprehensive data on traditionally utilized information were compiled, and herbarium materials were prepared. These materials have been deposited at the Iğdır National Wild Life Museum (INWM), affiliated with Iğdır University. Result and Discussion: This research identified a total of 58 taxa of medicinal plants belonging to 31 families. Among these, 50 species were found to grow naturally, while 8 species were cultivated. The predominant plant families included Asteraceae (8), Apiaceae (4), Lamiaceae (4), and Rosaceae (4). Infusion emerged as the most widely employed preparation method. The practice of traditional medicine remained prevalent among the population in Ağrı. Nevertheless, with the increasing availability of health services in the region, herbal medicine appeared to be more closely associated with healthcare and illness prevention than with curative purposes. The influx of new immigrants also contributed to the erosion of traditional knowledge. Notably, there is a discernible decline in traditional knowledge regarding the use of medicinal plants, both among younger generations and due to migration. Furthermore, this research serves as a foundational resource for prospective scientific inquiries aimed at the development of novel commercial drugs derived from plant sources.
RDL Kalıcı Kaydıİncele - ArticleAçık Erişim2024Formulation of hesperidin-loaded in situ gel for ocular drug delivery: a comprehensive study
Gözcü, Sefa; Polat, Heybet Kerem; Gültekin, Yakup; Ünal, Sedat; Karakuyu, Nasıf Fatih; Şafak, Esra Köngül; Doğan, Osman; Pezik, Esra; Haydar, Muhammet Kerim; Aytekin, Eren
BACKGROUND: Allergic conjunctivitis is one of the most common eye disorders. Different drugs are used for its treatment. Hesperidin is an active substance isolated from Citrus sinensis L. (Rutaceae) fruit peels, with known anti-inflammatory activity but low solubility. It was complexed with cyclodextrin and encapsulated in situ gel to extend its duration in the eye. RESULTS: The optimized formulation comprised 1% hesperidin, 1.5% hydroxyethyl cellulose, and 16% poloxamer 407. The viscosity at 25 °C was 492 ± 82 cP, and at 35 °C it was 8875 ± 248 cP, the pH was 7.01 ± 0.03, gelation temperature was 34 ± 1.3 °C, and gelation time was 33 ± 1.2 s. There was a 66% in vitro release in the initial 2 h, with a burst effect. A lipoxygenase (LOX) inhibition test determined that hesperidin was active at high doses on leukotyrens seen in the body in allergic diseases. In cell-culture studies, the hesperidin cyclodextrin complex loaded in situ gel, BRN9-CD (poloxamer 16%, hydroxy ethyl cellulose (HEC) 1.5%), enhanced cell viability in comparison with the hesperidin solution. It was determined that BRN9-CD did not cause any irritation in the ocular tissues in the Draize test. CONCLUSION: The findings of this study demonstrate the potential of the in situ gel formulation of hesperidin in terms of ease of application and residence time on the ocular surface. Due to its notable LOX inhibition activity and positive outcomes in the in vivo Draize test, it appears promising for incorporation into pharmaceutical formulations.
RDL Kalıcı Kaydıİncele